The invention discloses an anti-
keratin antibody detection method and an anti-
keratin antibody regent box, and belongs to the method for detecting the characteristics of blood in body. The method provided by the invention can be operated as follows: the
esophagus of a bandicoot can be made into a biological thin slice which can be taken as an
antigen and coated in the reaction region of a piece of slide glass;
blood serum to-be-detected is added in the reaction region and then added with complement after the incubation, rinsing and
drying in a spanning way, then added with the fluorescent markers of anti-complement antibodies after the incubation, rinsing and
drying in a spanning way, and then observed under a
fluorescence microscope after the incubation, rinsing and
drying in a spanning way; if the
horny layer appears typical and regular line-shaped or lamellar
fluorescence, the detection result is positive. The regent box provided by the utility model is filled with regents,
cover glass and slide glass with biological thin slices, such as
negative control serum,
positive control serum,
condensed phosphate buffer solution and the application solution of the fluorescent markers of complement and anti-complement antibodies. The method provided by the invention is better than the prior method in indicators such as sensitivity, specificity, negative
predictive value and positive predicative value, therefore, the method has higher diagnosis value in diagnosing rheumatoid diseases.