86results about How to "Achieving Targeted Therapy" patented technology

The present invention provides are a gold nanoflower structure and a preparation method therefor. The gold nanoflower structure is a gold nanoflower particle, with round-head columns being uniformly distributed at the periphery thereof, obtained by using gold octahedrons, gold balls or gold tetrahedrons as seed crystals and reducing chloroauric acid by using weak reductant in an environment of high-concentration polyvinylpyrrolidone. In addition, also provided in the present invention are a gold nanoflower/quantum dot composite probe for living cell immunofluorescent labeling and photothermal therapy, a preparation method therefor and a use thereof. In comparison with traditional probes, the probe, incorporates the features of photothermal therapy and fluorescent labeling, and is capable of killing cancer cells in an effective and directional way. Two light sources are adopted to bring a tremendous photothermal conversion efficiency and a greater enhancement on fluorescence intensity of quantum dots respectively, thus mutual interference of two effects are avoided tactfully. The coating of silicon dioxide averts the biotoxicity of the gold nanoflower and the quantum dot effectually, enabling the surface of the composite probe to be easily functionalized and also imparting an extraordinarily excellent biocompatibility to the composite probe.

The invention discloses a preparation based on gold, silver, a gold-silver mixture and glutathione/chitosan, and an application of the preparation. According to the preparation and the application, the precious metal gold and silver nano medicinal preparation prepared by in-situ biosynthesis of targeted pathological cells/tissues has excellent biocompatibility, good permeability and high target performance, can achieve high-space-time resolution and accurate location of nidus parts such as tumors, and can facilitate apoptosis of tumor cells; the relevant precious metal preparation can target pathological loca such as the tumor cells/tissues in an organism, effectively facilitate the apoptosis of the tumor cells and ablation of the tumor tissues, and serve as the potential medicinal preparation for early treatment of the tumors clinically; and the relevant preparation and the application of the preparation are simple and easy, good in targeted treatment effect, low in toxicity, and visual in result, can conduct polymorphic, multi-mode, real-time, dynamic, and clinical monitoring and treatment, and have wide medical clinical application values and prospects.

The invention provides sgRNA or PTG (Polycistronic tRNA-gRNA), through combination with a CRISPR-Cas9 technique, a T cell antigen receptor (TCR) can be knocked out, or one or more of genes relevant tomajor histocompatibility complex (MHCI) and immunosuppression molecules can be knocked out. The invention also provides a general type CAR-T cell which can express chimeric antigen receptor (CAR) butcannot express TCR, and a preparation method of the general type CAR-T cell. The general type CAR-T cell prepared by the preparation method is suitable for heterogeneity antigen, has generality and has higher killability at the same time.

The invention discloses a beta-lactamase-RGD-fusion protein and a preparation method thereof. The amino acid sequence of the recombinant beta-lactamase-RGD-fusion protein comprises a protein sequence which is derived from beta-lactamase and a sequence which comprises short peptide RGD. The recombinant beta-lactamase-RGD-fusion protein not only has the structure and the function of the beta-lactamase, but also has cell adhesion activity, so that the fusion protein can be positioned at the place of the tumor, thereby having better target. The beta-lactamase-RGD-fusion protein can be used for the treatment of the antibody guide enzyme prodrug, can be used for treating a series of solid tumors such as breast cancer and the like jointly with the cephalosporins prodrug, and has better effect than single melphalan and low toxicity.

The invention provides a fluorouracil-dextran conjugate and its preparing process, which comprises, derivatizing fluorouracil, introducing acetic acid groups onto the N1 position of fluorouracil, carrying out esterification reaction by utilizing the hydroxyl on the carboxyl and the dextran, thus obtaining fluorouracil-dextran macromolecular precursor medicament.

The invention relates to a cell membrane coated ultra-small ferroferric oxide nanocluster, and a preparation method and an application thereof. The method comprises the following steps: preparation ofultra-small ferroferric oxide nanoparticles, preparation of surface aminated ultra-small ferroferric oxide nanoparticles, preparation of ferroferric oxide nanoclusters, preparation of adriamycin-loaded ferroferric oxide nanoclusters, preparation of a cell suspension, and preparation of cell membrane coated ultra-small ferroferric oxide nanoclusters. After the nanocluster provided by the inventionis injected into a mouse body through a tail vein, T1/T2 bimodal MR imaging conversion can be achieved; meanwhile, the anti-tumor effect can be exerted; the chemotherapy effect of the nanocluster canbe further enhanced through a UTMD auxiliary means; and the nanocluster has potential clinical application value.

The invention provides a preparation carried with particles of antitumor drug and a preparation method thereof, belonging to the technical field of medicine. The preparation uses multidrug resistant mesenchymal stem cells as carrier of particles of antitumor drug, the multidrug resistant mesenchymal stem cells are multidrug resistant mesenchymal stem cells of a P-glycoprotein-expressing. According to the invention, the problem of necrocytosis of mesenchymal stem cells caused by antineoplastic drug accumulation is solved well, the particles of antitumor drug can be transported to a malignant tumor and released slowly in local, so that the targeted therapy of tumor is realized, curative effect is improved, toxic and side effect is reduced, and the invention has a wide application prospect.

The invention discloses a mesoporous silica nano-drug carrier and a preparation method thereof. The mesoporous silica nano-drug carrier mainly comprises calcium phosphate and silicon dioxide of whichthe mass percent ratio is 1-1.2 to 2.9-25.8. As calcium phosphate is doped with a mesoporous silica skeleton, the -Si-O-Si-bond density is reduced, and the biodegradability of mesoporous silica is improved; besides, the characteristic that calcium phosphate is stable under a neutral condition and easily soluble under an acid condition is utilized, so that drug release has the pH response characteristic, and drug targeted therapy is realized; on the other hand, dissolution of calcium phosphate enables pyrolysis of silica carrier, and degradation and excretion of the carrier are more facilitated.

The invention discloses a traditional Chinese medicine composition for treating infectious diseases of the genital system. The traditional Chinese medicine composition comprises 25 parts of Iris tectorum Maxim., 25 parts of radix sophorae flavescentis, 15 parts of Chinese knotweed herb, 15 parts of rhizoma chuanxiong, 15 parts of olibanum, 15 parts of myrrh, 15 parts of radix paeoniae rubra, 15 parts of salviae miltiorrhizae, 15 parts of radix clematidis, 15 parts of cinnamon, 10 parts of angelica sinensis, 10 parts of herba epimedii, 10 parts of cynomorium, 10 parts of herba cistanche, 5 parts of flos carthami, 5 parts of saussurea involucrata, 5 parts of malt selenium and 10 parts of borneol. In addition, the invention further discloses carbon-based paste for treating infectious diseases of the genital system and a preparation method and application of the carbon-based paste. The carbon-based paste is attached onto the perineum to treat the infectious diseases of the genital system through perineum administration, so that compared with a traditional oral medicine, the carbon-based paste is easier to absorb by the genital system, has the functions of detoxifying, sterilization, itching relief, pain relief, dehumidification, necrotic tissue removal, tissue regeneration promotion, inflammation resistance, cancer resistance, Qi tonifying and Yang generation, body resistance strengthening and consolidating, and Yin-Yang regulation, and realizes external treatment and prevention of internal infectious diseases of the genital system.

The invention discloses a schisanlactone E targeted drug delivery system. The system comprises schisanlactone E, Prussian blue nanoparticles, a biomimetic membrane wrapping layer and a hyaluronic acid modification layer. The invention also discloses a preparation method of the targeted drug delivery system. The preparation method comprises the following steps: synthesizing Prussian blue nanoparticles; preparing a biomimetic membrane; preparing Prussian blue loaded schisanlactone E nanoparticles from Prussian blue nanoparticles and schisanlactone E; preparing biological biomimetic membrane coated Prussian blue loaded hemoglobin nanoparticles by using the biomimetic membrane and the Prussian blue loaded schisanlactone E nanoparticles; and performing hyaluronic acid modification to obtain the schisanlactone E targeted drug delivery system. The schisanlactone E targeted drug delivery system can accurately deliver schisanlactone E to the attack part of arthritis so as to prolong the retention time of the drug at the attack part of arthritis and ensure the safety and effectiveness of treatment. Furthermore, the invention further discloses application of the schisanlactone E targeted drug delivery system in preparation of anti-rheumatoid arthritis drugs.

The invention discloses a preparation method of a calcium phosphate-rapamycin composite drug, a making method of a drug coating balloon and the drug coating balloon. Through compounding of calcium phosphate and rapamycin, the stability of a rapamycin structure can be improved, continous release of the rapamycin structure is facilitated in vivo, the adhesion capacity of rapamycin on the surface of the balloon and the infiltration capacity of rapamycin in tissue are improved, the retention time of the drug after administration is prolonged, the inhibition effect of rapamycin on cells is improved, so that the treatment effect of rapamycin on vascular restenosis diseases is enhanced, the solubility and stability of the drug can be improved by the nano-material coating, the use dosage of the drug is further reduced, and toxic and side effects are relieved or avoided; and due to the slow release effect of the nanoparticles, the peak-valley phenomenon of the blood concentration can be eliminated, a certain treatment concentration is maintained, and adverse reactions caused by instantaneous overhigh blood concentration are effectively prevented; and the nanoparticles can prolong the retention time of the drug in the circulation system and improve the treatment level of the drug.

The present invention belongs to the field of pharmaceutical preparations, and particularly relates to a RSPO1-containing targeted drug delivery system, preparation and applications thereof, wherein the RSPO1-containing targeted drug delivery system contains a RSPO1 conjugating carrier molecule, a drug and drug carrier particles, wherein RSPO1 is on the surface of the drug carrier particle. According to the present invention, the RSPO1-containing targeted drug delivery system can significantly improve the targeting property of the drug and the tumor treatment effect on the basis of the reduction of the toxic-side effects of the drug.

The invention discloses a novel erlotinib-chitosan-mesoporous silica nanoparticle-indocyanine green (ECMI) nanometer therapeutic agent and a preparation method and application thereof. The nanometer therapeutic agent uses mesoporous silica nanoparticles loaded with indocyanine green as carriers in the interior, utilizes ZnO QDs to block the pores on the surfaces of the mesoporous silica nanoparticles, and coats the surfaces of the mesoporous silica nanoparticle with an erlotinib-modified chitosan disulfide crosslinked polymer. The nanometer therapeutic agent can identify lung cancer cells belonging to different molecular classes and is used for controllable near-infrared fluorescence imaging. Through molecular targeting to drugs and photodynamic therapy, the therapeutic effect on lung cancer is improved.

The invention discloses a coumarin compound with BRD (bromodomain-containing protein 4) inhibiting effects. The coumarin compound comprises a compound with a structure shown as the formula (I) and medically acceptable salts thereof. The coumarin compound in a brand-new structure containing coumarin nucleuses can inhibit BRD4 to various degrees and accordingly can be applied to preparing BRD4 inhibitors or to preventing and treating BRD4 activity-related diseases, and due to a huge variety of the related diseases, can be extremely wide in application range. The invention also discloses a preparation method of the coumarin compound. The preparation method of the coumarin compound is rich in material resources, simple in operation, mild in condition, low in cost and highly applicable large-scale industrial production.

The invention provides a tumor microenvironment response type gene nano-micelle. The tumor microenvironment response type gene nano-micelle comprises polyethyleneimine-phenylboronic acid and polyethyleneimine-maleic anhydride-target radical, wherein the mass ratio of the polyethyleneimine-phenylboronic acid to the polyethyleneimine-maleic anhydride-target radical ranges from (1 to 1) to (1 to 2).The invention further provides a preparation method of the tumor microenvironment response type gene nano-micelle. The invention further provides application of the tumor microenvironment response type gene nano-micelle to preparation of a medicine for treating cancers and application of the tumor microenvironment response type gene nano-micelle serving as a medicine carrier. The nano-micelle provided by the invention can efficiently load and transmit RNA (Ribonucleic Acid) and a medicine with a microenvironment response type gene enters a tumor cell target to play a role; the tumor microenvironment response type gene nano-micelle is especially suitable for loading the RNA for carrying out gene therapy on malignant tumor. The preparation method is simple and convenient and is suitable forlarge-scale production.

The invention provides a composite probiotic preparation for targeting treatment on tumor and a preparation method of the composite probiotic preparation. By adopting the technical scheme, on the basis of physiological actions of various probiotics and synergic relationships of the probiotics, bifidobacterium adolescentis, lactobacillus crispatus, lactobacillus jensenii, lactobacillus vaginalis and lactobacillus johnsonii are adopted as preparation main bodies, on the basis, a compounding scheme is designed from angles of amount proportioning, numbers of viable organisms, and the like, and aneffective preparation method is provided. By adopting the preparation, targeting treatment on tumor can be achieved in an oral taking mode, and meanwhile the preparation can have synergetic effects with other anti-tumor medicines to promote anti-tumor effects of the preparation. The preparation is safe and non-toxic, free of conspicuous side effect, free of limits of tumor sizes and types, and good in quantitative treatment effect on various types of tumor.

The invention provides an FEN1-targeted 19F-MR/fluorescence multi-mode molecular imaging and drug-loaded breast cancer diagnosis and treatment integrated nanoprobe and a preparation method and application thereof. The probe is nanoparticles formed by coating a perfluorinated carbon carrier with a mixture of a surfactant containing a FEN1-targeted small molecule inhibitor SC13 or a derivative thereof and a fluorescent dye; and a mixed solution is uniformly dispersed in water and glycerin, ultrasonic treatment is performed to remove uncoated components, and purifying is performed to obtain the drug-loaded nanoparticles for 19F-MR imaging. The probe can realize in-vivo 19F-MR molecular imaging and optical imaging, and realize molecular level accurate diagnosis of solid tumors such as breast cancer; not only can the effect of targeted combination diagnosis of the solid tumors be achieved, but also targeted treatment can be achieved; and by means of the characteristic that perfluoro in thenucleus can carry and release a large amount of oxygen, the hypoxic microenvironment in the tumors is improved, and the chemotherapy sensitization effect and diagnosis and treatment of the solid tumors are integrated.