86results about How to "Achieving Targeted Therapy" patented technology

The present invention provides are a gold nanoflower structure and a preparation method therefor. The gold nanoflower structure is a gold nanoflower particle, with round-head columns being uniformly distributed at the periphery thereof, obtained by using gold octahedrons, gold balls or gold tetrahedrons as seed crystals and reducing chloroauric acid by using weak reductant in an environment of high-concentration polyvinylpyrrolidone. In addition, also provided in the present invention are a gold nanoflower / quantum dot composite probe for living cell immunofluorescent labeling and photothermal therapy, a preparation method therefor and a use thereof. In comparison with traditional probes, the probe, incorporates the features of photothermal therapy and fluorescent labeling, and is capable of killing cancer cells in an effective and directional way. Two light sources are adopted to bring a tremendous photothermal conversion efficiency and a greater enhancement on fluorescence intensity of quantum dots respectively, thus mutual interference of two effects are avoided tactfully. The coating of silicon dioxide averts the biotoxicity of the gold nanoflower and the quantum dot effectually, enabling the surface of the composite probe to be easily functionalized and also imparting an extraordinarily excellent biocompatibility to the composite probe.

The invention belongs to the field of medicine preparation, and in particular to a targeting nanometer drug delivery system aiming at glioma, and a preparation method and application thereof. The drug delivery system comprises target functional molecules, a drug and nano carriers. The target functional molecules are from short chain polypeptide from interleukin 13; the drug is a micromolecular anti-glioma drug; the nano carriers are liposome with surface modified by polyethylene glycol, nanoparticles, polymeric vesicles, polymer micelles and solid lipid nanoparticles; and the drug is enveloped in the nano carriers in an enveloping or covalent connection manner, and the short chain polypeptide is connected with the polyethylene glycol on the surfaces of the nanoparticles through covalent connection. The drug delivery system can promote uptake of the glioma cells by mediated effect of an interleukin 13 receptor alpha 2 on the surfaces of the glioma cells, so as to improve the effect of anti-glioma chemotherapeutics.

The invention discloses a preparation based on gold, silver, a gold-silver mixture and glutathione / chitosan, and an application of the preparation. According to the preparation and the application, the precious metal gold and silver nano medicinal preparation prepared by in-situ biosynthesis of targeted pathological cells / tissues has excellent biocompatibility, good permeability and high target performance, can achieve high-space-time resolution and accurate location of nidus parts such as tumors, and can facilitate apoptosis of tumor cells; the relevant precious metal preparation can target pathological loca such as the tumor cells / tissues in an organism, effectively facilitate the apoptosis of the tumor cells and ablation of the tumor tissues, and serve as the potential medicinal preparation for early treatment of the tumors clinically; and the relevant preparation and the application of the preparation are simple and easy, good in targeted treatment effect, low in toxicity, and visual in result, can conduct polymorphic, multi-mode, real-time, dynamic, and clinical monitoring and treatment, and have wide medical clinical application values and prospects.

The invention relates to an environmental response type anti-tumor nanometer medicine with high medicine loading capacity, a carrier and a preparation method thereof. The nanometer medicine comprisesan amphiphilic polymer carrier and a medicine prodrug; the amphiphilic polymer carrier comprises a hydrophilic segment and a hydrophobic segment which are connected mutually; the hydrophilic segment comprises polyethylene glycol or poly(N-(2-hydroxypropyl)methacrylamide); the hydrophobic segment comprises D-alpha-tocopherol succinate; the medicine prodrug comprises a medicine for treating tumor aswell as a hydrophobic chain segment which is connected with the medicine through a chemical bond; and the hydrophobic chain segment comprises D-alpha-tocopherol succinate. The nanometer medicine hasthe characteristics of high medicine loading capacity, environmental responsiveness and adjustable and controllable grain size, and can achieve the properties that the tumor penetration ability is high, the tumor cell multidrug resistance is achieved, drug-resistant cells are killed in advance, tumor related fibroblast is not killed and tumor stem cells can be killed effectively according to composition of different polymers and the loaded prodrug molecule types.

The invention provides sgRNA or PTG (Polycistronic tRNA-gRNA), through combination with a CRISPR-Cas9 technique, a T cell antigen receptor (TCR) can be knocked out, or one or more of genes relevant tomajor histocompatibility complex (MHCI) and immunosuppression molecules can be knocked out. The invention also provides a general type CAR-T cell which can express chimeric antigen receptor (CAR) butcannot express TCR, and a preparation method of the general type CAR-T cell. The general type CAR-T cell prepared by the preparation method is suitable for heterogeneity antigen, has generality and has higher killability at the same time.

The invention discloses a beta-lactamase-RGD-fusion protein and a preparation method thereof. The amino acid sequence of the recombinant beta-lactamase-RGD-fusion protein comprises a protein sequence which is derived from beta-lactamase and a sequence which comprises short peptide RGD. The recombinant beta-lactamase-RGD-fusion protein not only has the structure and the function of the beta-lactamase, but also has cell adhesion activity, so that the fusion protein can be positioned at the place of the tumor, thereby having better target. The beta-lactamase-RGD-fusion protein can be used for the treatment of the antibody guide enzyme prodrug, can be used for treating a series of solid tumors such as breast cancer and the like jointly with the cephalosporins prodrug, and has better effect than single melphalan and low toxicity.

The invention provides a fluorouracil-dextran conjugate and its preparing process, which comprises, derivatizing fluorouracil, introducing acetic acid groups onto the N1 position of fluorouracil, carrying out esterification reaction by utilizing the hydroxyl on the carboxyl and the dextran, thus obtaining fluorouracil-dextran macromolecular precursor medicament.

The invention discloses a medicine aptamer constructed by nucleoside analogue medicine molecules, a preparation method and application thereof. Structure units similar to the structures in the traditional aptamer sequence can be respectively replaced by utilizing nucleoside analogue medicine molecules with different structures through the medicine aptamer, thereby obtaining a medicine-containing oligonucleotide sequence with similar targeting functions. A series of medicine aptamers targeting to different tumors can be prepared by selecting aptamer sequences with different targeting receptors,and a universal novel precise targeted medicine is constructed for treating different types of tumors. The preparation method comprises the following steps of converting the nucleoside analogue medicine molecules with structures similar to the basic structural unit of nucleotide into the corresponding phosphoramidite monomers, and synthesizing a series of medicine aptamers with precise compositions and different sequences by utilizing solid-phase synthesis technology. The medicine aptamer disclosed by the invention has an original targeting function corresponding to the aptamer, thereby realizing specific targeting of different tumors, reaching a good medicine delivery effect and greatly improving therapeutic effect.

The invention relates to a cell membrane coated ultra-small ferroferric oxide nanocluster, and a preparation method and an application thereof. The method comprises the following steps: preparation ofultra-small ferroferric oxide nanoparticles, preparation of surface aminated ultra-small ferroferric oxide nanoparticles, preparation of ferroferric oxide nanoclusters, preparation of adriamycin-loaded ferroferric oxide nanoclusters, preparation of a cell suspension, and preparation of cell membrane coated ultra-small ferroferric oxide nanoclusters. After the nanocluster provided by the inventionis injected into a mouse body through a tail vein, T1 / T2 bimodal MR imaging conversion can be achieved; meanwhile, the anti-tumor effect can be exerted; the chemotherapy effect of the nanocluster canbe further enhanced through a UTMD auxiliary means; and the nanocluster has potential clinical application value.

The invention relates to a tumor-targeted zinc-based metal-organic framework drug carrier. The drug carrier comprises a zinc-based metal-organic framework and folic acid molecules, wherein the zinc-based metal-organic framework is formed by a solvent thermal reaction between zinc nitrate and a 2-amino-1,4-terephthalic acid and has a molecular formula of Zn4O (C8H5NO4)3, and the folic acid molecules are coupled to the zinc-based metal-organic framework through a modification method after synthesis. The invention also provides a preparation method of the tumor-targeted zinc-based metal-organic framework drug carrier. The drug carrier disclosed by the invention can be prepared from raw materials wide in sources, the preparation method is simple and the drug loading capacity is extremely high. The tumor-targeted zinc-based metal-organic framework drug carrier disclosed by the invention has a dual function of passive targeting of EPR (Electron Paramagnetic Resonance) and active targeting of folic acid so that the drug-carried tumor-targeted zinc-based metal-organic framework is effectively targeted and concentrated in a tumor site, thereby achieving an aim of targeted treatment and remarkably reducing the dosage and toxic and side effects of an anti-tumor drug.

The invention belongs to the biological technical field, and relates to a targeting nano drug delivery system aiming at brain glioma modified by short peptides of a low density lipoprotein receptor, and a preparation method and application thereof. The drug delivery system comprises target functional molecules, a drug and nano carriers. The target functional molecules are from the short peptides of the low density lipoprotein receptor, obtained by phage display technology. The drug is enveloped in the nano carriers in an enveloping or covalent connection manner, and the short peptides are connected with the polyethylene glycol on the surfaces of the nanoparticles through covalent connection. The drug delivery system can invade and immerse tumor cells by hemato encephalic barrier, can enter into the tumor cells by EPR (enhanced permeability and retention effect), and can promote uptake of brain glioma cells by mediated effect of the low density lipoprotein receptor on the surfaces of the glioma cells so as to improve the effect of anti-brain glioma chemotherapeutics.

The invention provides a preparation carried with particles of antitumor drug and a preparation method thereof, belonging to the technical field of medicine. The preparation uses multidrug resistant mesenchymal stem cells as carrier of particles of antitumor drug, the multidrug resistant mesenchymal stem cells are multidrug resistant mesenchymal stem cells of a P-glycoprotein-expressing. According to the invention, the problem of necrocytosis of mesenchymal stem cells caused by antineoplastic drug accumulation is solved well, the particles of antitumor drug can be transported to a malignant tumor and released slowly in local, so that the targeted therapy of tumor is realized, curative effect is improved, toxic and side effect is reduced, and the invention has a wide application prospect.

The invention provides a cubic cyclodextrin framework-RGD composition (RGD-COF) and a preparation method thereof. Specifically, the cyclodextrin framework-RGD composition of the present invention contains a cyclodextrin framework (COF) having a cubic structure and RGD. The cubic cyclodextrin framework-RGD composition disclosed by the invention can avoid phagocytosis and elimination of macrophages,enhance the mobility and the adhesion to damaged blood vessels and efficiently target and gather at activated blood platelets at the damaged blood vessel parts, and has great application prospects ontargeted diagnosis and treatment of vascular related diseases such as out-of-control hemorrhage, atherosclerosis and cerebral apoplexy. The invention provides a nanoscale cubic cyclodextrin framework-RGD composition which can be used for intravenous injection or a micron-scale cubic cyclodextrin framework-RGD composition which can be used for local external application by utilizing the advantagethat the size of a cyclodextrin-metal organic framework (CD-MOF) is controllable.

The invention discloses a mesoporous silica nano-drug carrier and a preparation method thereof. The mesoporous silica nano-drug carrier mainly comprises calcium phosphate and silicon dioxide of whichthe mass percent ratio is 1-1.2 to 2.9-25.8. As calcium phosphate is doped with a mesoporous silica skeleton, the -Si-O-Si-bond density is reduced, and the biodegradability of mesoporous silica is improved; besides, the characteristic that calcium phosphate is stable under a neutral condition and easily soluble under an acid condition is utilized, so that drug release has the pH response characteristic, and drug targeted therapy is realized; on the other hand, dissolution of calcium phosphate enables pyrolysis of silica carrier, and degradation and excretion of the carrier are more facilitated.

The invention discloses the preparation and application of a target mitochondrial nanoparticle based on a composite porcelain plastid. It uses nano-porcelain as a drug carrier, and modifies targeted small molecules on its surface after embedding the drug by self-assembly method. The nanoporcelain has stable structure, time stability and structural stability, and the drug embedded in it can be released slowly. After surface modification of targeted small molecules, nanoparticles can achieve targeted slow release. In cells, nanoparticles achieve a good effect of targeting organelles. It has important application significance for chronic diseases such as Kinson's disease and cancer that require long-term treatment.

The invention provides a CAR-T cell and a preparation method. The CAR-T cell restrains a T cell antigen receptor (TCR), a major histocompatibility complex (MHCI) and an immunosuppression molecule related gene. The CAR-T cell expresses a chimeric antigen receptor (CAR), does not express the TCR, MHCI and an immunosuppression molecule PD1, is suitable for a heterogenous receptor, has generality, andmeanwhile has higher killability.

The invention discloses application of a noncoding protein gene in the diagnosis and treatment of liver cancer, and the specific noncoding protein gene is LINC01703. The invention discovers for the first time that LINC01703 is related to the incidence and development of liver cancer. By bioinformatic analysis, the invention discovers that LINC01703 has relatively high AUC value in patients with liver cancer, indicating that LINC01703 has relatively high accuracy and specificity as a diagnostic marker for liver cancer. Through an experiment, the invention verifies that changing the expression level of LINC01703 can influence the proliferation and apoptosis and the like of liver cancer, indicating that LINC01703 can be used as a drug target in the treatment of liver cancer.

The invention discloses a traditional Chinese medicine composition for treating infectious diseases of the genital system. The traditional Chinese medicine composition comprises 25 parts of Iris tectorum Maxim., 25 parts of radix sophorae flavescentis, 15 parts of Chinese knotweed herb, 15 parts of rhizoma chuanxiong, 15 parts of olibanum, 15 parts of myrrh, 15 parts of radix paeoniae rubra, 15 parts of salviae miltiorrhizae, 15 parts of radix clematidis, 15 parts of cinnamon, 10 parts of angelica sinensis, 10 parts of herba epimedii, 10 parts of cynomorium, 10 parts of herba cistanche, 5 parts of flos carthami, 5 parts of saussurea involucrata, 5 parts of malt selenium and 10 parts of borneol. In addition, the invention further discloses carbon-based paste for treating infectious diseases of the genital system and a preparation method and application of the carbon-based paste. The carbon-based paste is attached onto the perineum to treat the infectious diseases of the genital system through perineum administration, so that compared with a traditional oral medicine, the carbon-based paste is easier to absorb by the genital system, has the functions of detoxifying, sterilization, itching relief, pain relief, dehumidification, necrotic tissue removal, tissue regeneration promotion, inflammation resistance, cancer resistance, Qi tonifying and Yang generation, body resistance strengthening and consolidating, and Yin-Yang regulation, and realizes external treatment and prevention of internal infectious diseases of the genital system.

The invention relates to an inflammation targeted compound, a preparation method and application thereof, and belongs to the technical field of pharmacy. The compound comprises a part A and a part B, wherein the part A is inflammation related cells the number of which is 1*10<5>-1*10<8>; the part B is a treatment medicine, and the dosage is clinically acceptable effective treatment dosage. According to the preparation method, a cell-medicine compound is formed after the treatment medicines and a monocyte or neutrophil are incubated in vivo, is applied to the affected host, and is recruited to pathological part in the recruitment of inflammatory reactions of tumors, rheumatoid arthritis or atherosclerosis, so that targeted treatment to the diseases can be realized.

The invention discloses a schisanlactone E targeted drug delivery system. The system comprises schisanlactone E, Prussian blue nanoparticles, a biomimetic membrane wrapping layer and a hyaluronic acid modification layer. The invention also discloses a preparation method of the targeted drug delivery system. The preparation method comprises the following steps: synthesizing Prussian blue nanoparticles; preparing a biomimetic membrane; preparing Prussian blue loaded schisanlactone E nanoparticles from Prussian blue nanoparticles and schisanlactone E; preparing biological biomimetic membrane coated Prussian blue loaded hemoglobin nanoparticles by using the biomimetic membrane and the Prussian blue loaded schisanlactone E nanoparticles; and performing hyaluronic acid modification to obtain the schisanlactone E targeted drug delivery system. The schisanlactone E targeted drug delivery system can accurately deliver schisanlactone E to the attack part of arthritis so as to prolong the retention time of the drug at the attack part of arthritis and ensure the safety and effectiveness of treatment. Furthermore, the invention further discloses application of the schisanlactone E targeted drug delivery system in preparation of anti-rheumatoid arthritis drugs.

The invention discloses a preparation method of a calcium phosphate-rapamycin composite drug, a making method of a drug coating balloon and the drug coating balloon. Through compounding of calcium phosphate and rapamycin, the stability of a rapamycin structure can be improved, continous release of the rapamycin structure is facilitated in vivo, the adhesion capacity of rapamycin on the surface of the balloon and the infiltration capacity of rapamycin in tissue are improved, the retention time of the drug after administration is prolonged, the inhibition effect of rapamycin on cells is improved, so that the treatment effect of rapamycin on vascular restenosis diseases is enhanced, the solubility and stability of the drug can be improved by the nano-material coating, the use dosage of the drug is further reduced, and toxic and side effects are relieved or avoided; and due to the slow release effect of the nanoparticles, the peak-valley phenomenon of the blood concentration can be eliminated, a certain treatment concentration is maintained, and adverse reactions caused by instantaneous overhigh blood concentration are effectively prevented; and the nanoparticles can prolong the retention time of the drug in the circulation system and improve the treatment level of the drug.

The present invention belongs to the field of pharmaceutical preparations, and particularly relates to a RSPO1-containing targeted drug delivery system, preparation and applications thereof, wherein the RSPO1-containing targeted drug delivery system contains a RSPO1 conjugating carrier molecule, a drug and drug carrier particles, wherein RSPO1 is on the surface of the drug carrier particle. According to the present invention, the RSPO1-containing targeted drug delivery system can significantly improve the targeting property of the drug and the tumor treatment effect on the basis of the reduction of the toxic-side effects of the drug.

The invention discloses a novel erlotinib-chitosan-mesoporous silica nanoparticle-indocyanine green (ECMI) nanometer therapeutic agent and a preparation method and application thereof. The nanometer therapeutic agent uses mesoporous silica nanoparticles loaded with indocyanine green as carriers in the interior, utilizes ZnO QDs to block the pores on the surfaces of the mesoporous silica nanoparticles, and coats the surfaces of the mesoporous silica nanoparticle with an erlotinib-modified chitosan disulfide crosslinked polymer. The nanometer therapeutic agent can identify lung cancer cells belonging to different molecular classes and is used for controllable near-infrared fluorescence imaging. Through molecular targeting to drugs and photodynamic therapy, the therapeutic effect on lung cancer is improved.

The invention discloses a coumarin compound with BRD (bromodomain-containing protein 4) inhibiting effects. The coumarin compound comprises a compound with a structure shown as the formula (I) and medically acceptable salts thereof. The coumarin compound in a brand-new structure containing coumarin nucleuses can inhibit BRD4 to various degrees and accordingly can be applied to preparing BRD4 inhibitors or to preventing and treating BRD4 activity-related diseases, and due to a huge variety of the related diseases, can be extremely wide in application range. The invention also discloses a preparation method of the coumarin compound. The preparation method of the coumarin compound is rich in material resources, simple in operation, mild in condition, low in cost and highly applicable large-scale industrial production.

The invention discloses a cytolysosome-targeting anticancer cluster and a preparation method thereof. The anticancer cluster is prepared by mixing a small-molecular anticancer drug serving as a ligand with chloroauric acid and radiating a formed mixture with gamma rays. The prepared anticancer cluster is an 8e-structure anti-cancer nanocluster with precise atoms; the number of anti-cancer drugs in the nanocluster is precise, introduction of external lysosome targeting groups is not needed, the anti-cancer drugs can naturally penetrate through cell membranes to be enriched in lysosome, precise chemotherapy drug delivery is conducted, and cancer cells are effectively killed; and therefore, other diseases caused by excessive chemotherapeutic drugs in the body are avoided, and the nano-cluster directly synthesized by the anti-cancer drugs has an application value in realizing targeted quantitative administration and cancer treatment.

The invention provides a composite probiotic preparation for targeting treatment on tumor and a preparation method of the composite probiotic preparation. By adopting the technical scheme, on the basis of physiological actions of various probiotics and synergic relationships of the probiotics, bifidobacterium adolescentis, lactobacillus crispatus, lactobacillus jensenii, lactobacillus vaginalis and lactobacillus johnsonii are adopted as preparation main bodies, on the basis, a compounding scheme is designed from angles of amount proportioning, numbers of viable organisms, and the like, and aneffective preparation method is provided. By adopting the preparation, targeting treatment on tumor can be achieved in an oral taking mode, and meanwhile the preparation can have synergetic effects with other anti-tumor medicines to promote anti-tumor effects of the preparation. The preparation is safe and non-toxic, free of conspicuous side effect, free of limits of tumor sizes and types, and good in quantitative treatment effect on various types of tumor.

The invention provides a polypeptide with ovarian tumor targeted D-configuration and a gene delivery system thereof. The L-polypeptide YTRDLVYKDPARPKIQKTCTF inverted sequence is replaced by D-configuration amino acid so as to obtainD(FTCTKQIKPRAPDKYVLDRTV)sequence polypeptide as targeting molecules, and the gene is targeted and delivered to ovarian carcinoma cells by using the high compatibility with FSHR; and theD(FTCTKQIKPRAPDKYVLDRTY)is decorated to the PEI-PEG surface so as to obtain aD(FTCTKQIKPRAPDKYVLDRTY)-PEG-PEI(D-FP21-pp)gene vector, wherein the gene vector encapsulates a therapeutic gene Gro-oshRNA. The gene transfection efficiency can be greatly improved, and the anti-tumor rate of the naked mouse subcutaneous tumor can be remarkably improved.

The invention provides an FEN1-targeted 19F-MR / fluorescence multi-mode molecular imaging and drug-loaded breast cancer diagnosis and treatment integrated nanoprobe and a preparation method and application thereof. The probe is nanoparticles formed by coating a perfluorinated carbon carrier with a mixture of a surfactant containing a FEN1-targeted small molecule inhibitor SC13 or a derivative thereof and a fluorescent dye; and a mixed solution is uniformly dispersed in water and glycerin, ultrasonic treatment is performed to remove uncoated components, and purifying is performed to obtain the drug-loaded nanoparticles for 19F-MR imaging. The probe can realize in-vivo 19F-MR molecular imaging and optical imaging, and realize molecular level accurate diagnosis of solid tumors such as breast cancer; not only can the effect of targeted combination diagnosis of the solid tumors be achieved, but also targeted treatment can be achieved; and by means of the characteristic that perfluoro in thenucleus can carry and release a large amount of oxygen, the hypoxic microenvironment in the tumors is improved, and the chemotherapy sensitization effect and diagnosis and treatment of the solid tumors are integrated.