Preparation method of calcium phosphate-rapamycin composite drug, making method of drug coating balloon and drug coating balloon
A rapamycin and drug coating technology, which is applied in the field of calcium phosphate-rapamycin compound drug preparation, can solve the problem of short drug retention time, slow tissue absorption, and limited clinical pharmacology of rapamycin drug-coated balloons applications and biological effects
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Embodiment 1
[0054] A preparation method of calcium phosphate-rapamycin compound medicine of the present invention, comprises the following steps:
[0055] Step S1: adding rapamycin to DMEM culture solution so that the rapamycin content in the mixture of rapamycin and DMEM culture solution is 100 nmol / L, and fully equilibrating for 24 hours.
[0056] Step S2: Add 110 μL of CaCl with a concentration of 1 mol / L to the fully equilibrated solution in Step S1 2 A mineralization reaction is initiated to obtain a nanoparticle suspension. The entire reaction is performed in a cell culture environment. The reaction temperature was 37°C. Response environment CO 2 The content is 5%. Sodium citrate was added 0.5 hours after the reaction to terminate the reaction to control the size of nanoparticles in the nanoparticle suspension.
[0057] Implementation two:
[0058] A preparation method of calcium phosphate-rapamycin compound medicine of the present invention, comprises the following steps:
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Embodiment 3
[0062] A preparation method of calcium phosphate-rapamycin compound medicine of the present invention, comprises the following steps:
[0063] Step S1: Add rapamycin to DMEM culture medium, so that the content of rapamycin in the mixture of rapamycin and DMEM culture medium is 102 nmol / L, and fully equilibrate for 28 hours.
[0064] Step S2: Add 200 μL of CaCl with a concentration of 1 mol / L to the fully equilibrated solution in Step S1 2 A mineralization reaction is initiated to obtain a nanoparticle suspension. The entire reaction is performed in a cell culture environment. The reaction temperature was 39°C. Response environment CO 2 The content is 6%. Two hours after the reaction, sodium citrate was added to terminate the reaction to control the size of nanoparticles in the nanoparticle suspension.
Embodiment 4
[0066] A method for preparing a calcium phosphate-rapamycin composite drug-coated balloon of the present invention comprises the following steps:
[0067] Step (1): Take the calcium phosphate-rapamycin nanoparticle suspension prepared by any one of the calcium phosphate-rapamycin composite drug preparation methods above.
[0068] Step (2): coating the calcium phosphate-rapamycin composite drug coating on the surface of the balloon by ultrasonic spraying.
[0069] The ultrasonic spraying method includes the following steps:
[0070] a) The calcium phosphate-rapamycin nanoparticle suspension is placed in a syringe pump and sprayed onto the surface of the balloon through an ultrasonic nozzle. The nanoparticle suspension passed through the ultrasonic nozzle at a speed of 0.08 mL / min. Ultrasonic output power is 1.5W. Ultrasonic frequency is 30kHz.
[0071] b) Vacuum drying at 37°C for 2 hours after spraying. The drying environment is a vacuum environment.
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