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Inflammation targeted compound, preparation and application thereof

A compound and inflammation technology, which can be used in medical preparations with non-active ingredients, medical preparations containing active ingredients, anti-inflammatory agents, etc., and can solve problems such as liver and kidney damage.

Inactive Publication Date: 2014-05-07
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment of atherosclerosis is to reduce blood lipids by taking lipid-regulating drugs (such as: statins), or use anti-platelet adhesion and aggregation drugs (such as: aspirin) to prevent thrombosis, or use thrombolytic drugs and anticoagulants ( Such as: heparin) to prevent vascular restenosis, but these drugs cause great damage to liver and kidney functions, and long-term use will inevitably cause liver and kidney diseases

Method used

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  • Inflammation targeted compound, preparation and application thereof
  • Inflammation targeted compound, preparation and application thereof
  • Inflammation targeted compound, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this embodiment, monocytes or neutrophils are collected from human peripheral blood samples, and incubated with therapeutic drugs at a certain temperature to form a structure such as figure 1 Complexes are shown where the drug is inside the cell, adsorbed on the cell membrane and or embedded in the cell membrane bilayer. The culture medium is RPMI-1640 medium.

[0029] The specific preparation method is as follows:

[0030] Step 1. Collect mononuclear cells by density gradient centrifugation: extract heparin with a 10mL syringe, and push out the heparin after wetting the wall of the syringe. Collect venous blood sample 10mL. Take 10mL PBS, mix PBS and blood 1:1, take 10mL FICOLL and add it to another 50mL centrifuge tube, add the mixture of PBS and blood slowly along the tube wall, and centrifuge at 1800rpm for 30min, a white film-like mononuclear cell layer can be seen, The pasteurized tube slowly sucked the cell layer into another 15mL centrifuge tube, added 3mL...

Embodiment 2

[0042] The culture medium of this embodiment adopts RPMI-1640 medium, and the compound is prepared according to the following method:

[0043] Step 1. Collect mononuclear cells by immunomagnetic bead method: extract heparin with a 10mL syringe, push out the heparin after wetting the wall of the syringe, and collect 10mL of venous blood sample. Take 10 mL of PBS, mix PBS with blood 1:1, add 4 mL of red blood cell lysate, incubate at room temperature for 4 minutes, centrifuge at 1200 rpm for 10 minutes, remove the supernatant, add 2 mL of PBS to suspend finely, take 100 μL of anti-CD14 magnetic beads and add them to the cell suspension, 4 Incubate at ℃ for 15 min, add the cell suspension to the magnetic field, separate on the Stemsep separation column, collect the cells, and dilute them with 1.5×10 6 Inoculate in a culture flask at 37°C, 5% CO 2 Incubator cultivation.

[0044] Step 2, preparation of cell-drug complex: mononuclear cells were mixed with 1×10 6 , seeded in a 24-...

Embodiment 3

[0055] The culture medium of this embodiment adopts RPMI-1640 medium, and the compound is prepared according to the following method:

[0056] Step 1. Collect neutrophils by density gradient centrifugation: extract heparin with a 10mL syringe, push out the heparin after wetting the wall of the syringe, and collect 10mL of venous blood sample. Mix 10mL Hanks with blood at a ratio of 1:1, take 2mL of centriole cell separation solution and add it to another 50mL centrifuge tube, add the mixture of Hanks and blood slowly along the tube wall, centrifuge at 900rpm for 30min, and the centrifuge tube is divided into 6 layers: plasma, single Nuclear cell layer, separation solution, neutrophils, the rest of the separation solution, erythrocyte sedimentation layer, absorb the neutrophil layer and the separation solution layer below it, transfer to another 15mL centrifuge tube, add 3mL Hanks , centrifuged at 1200rpm for 10min. Discard the supernatant, add 10mL PBS, mix well and centrifug...

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Abstract

The invention relates to an inflammation targeted compound, a preparation method and application thereof, and belongs to the technical field of pharmacy. The compound comprises a part A and a part B, wherein the part A is inflammation related cells the number of which is 1*10<5>-1*10<8>; the part B is a treatment medicine, and the dosage is clinically acceptable effective treatment dosage. According to the preparation method, a cell-medicine compound is formed after the treatment medicines and a monocyte or neutrophil are incubated in vivo, is applied to the affected host, and is recruited to pathological part in the recruitment of inflammatory reactions of tumors, rheumatoid arthritis or atherosclerosis, so that targeted treatment to the diseases can be realized.

Description

technical field [0001] The invention relates to a compound targeting inflammation, its preparation method and application, and belongs to the technical field of pharmacy. Background technique [0002] Inflammatory response, commonly known as inflammation, refers to the physiological response of biological tissue stimulated by trauma, bleeding, or pathogenic infection. Long-term chronic inflammation can cause a series of diseases, such as hay fever, periodontitis, atherosclerosis, rheumatoid arthritis, and even cancer. [0003] The latest research shows that inflammation is considered to be the seventh major feature of tumor diseases. The occurrence and development of various tumors are related to inflammation. Inflammation-related tumors account for about 19% of all malignant tumors, and more than 2 million people around the world develop inflammation-related tumors every year. In the tumor tissue area, both tumor cells and inflammatory cells can produce a variety of chemo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K47/46A61P35/00A61P29/00A61P19/02A61P9/10
Inventor 靳翔
Owner ZHEJIANG UNIV OF TECH
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