Composite probiotic preparation for targeting treatment on tumor and preparation method of composite probiotic preparation
A tumor targeting and bacterial agent technology, applied in the field of microorganisms, can solve the problems of physical injury, systemic toxicity, weak specificity, etc., and achieve the effect of reducing side effects and promoting anti-tumor effect.
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Embodiment 1
[0025] A compound probiotic bacterial agent for targeted tumor therapy and a preparation method thereof, comprising Bifidobacterium adolescent, Lactobacillus crispatus, Lactobacillus jansii, vaginal Lactobacillus and Lactobacillus johnsonii; the number of viable bacteria in the compound probiotic bacterial agent is 10 9 pcs / g.
[0026] A preparation method of the above-mentioned composite probiotic bacterial agent, comprising the following steps:
[0027] 1) Bacteria selection and activation: five kinds of probiotics were selected for medium activation;
[0028] 2) Mixing and centrifugation: the bacteria solution obtained in step 1) is mixed according to the volume ratio of 1:1:1:1:1 and then centrifuged to obtain the bacteria;
[0029] 3) Freeze-drying: adding a freeze-dried powder protective agent to the cells obtained in step 2), and freeze-drying in a freeze-drying machine.
[0030] On the basis of the above technical solutions, the following conditions are met:
[003...
Embodiment 2
[0037] A compound probiotic bacterial agent for targeted tumor therapy and a preparation method thereof, comprising Bifidobacterium adolescent, Lactobacillus crispatus, Lactobacillus jansii, vaginal Lactobacillus and Lactobacillus johnsonii; the number of viable bacteria in the compound probiotic bacterial agent is 10 9 pcs / g.
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