9 results about "Cytolysosome" patented technology

The application discloses a pinacine alkyl tetrahydrocarbazole fluorescent probe capable of detecting cell lysosome polarity and a preparation method and application thereof. 9-(3-(dimethylamino)propyl)-3,3-dimethyl-2,3,4,9-tetrahydro-1H-2,4-bridged methylene carbazole-6-formaldehyde is used as raw material, and condensation reaction is carried out with 1,3-indan dione to obtain compound 2-(2-(9-(3-(dimethylamino)propyl)-3,3-dimethyl-2,3,4,9-tetrahydro-1H-2,4-bridged methylene carbazole-6-yl)vinyl)-1-indene-1,3(2H)-dione (TC-AP-ID), and the probe can specifically detect the polarity of cell lysosomes. In a mixed system of 1,4-dioxane and water, with the increase of the polarity of the system, the fluorescence color of the probe gradually changes from orange to red, and the fluorescence intensity significantly decreases. The probe has the advantages of simple synthesis, good selectivity, high sensitivity, good biocompatibility, low toxicity and the like, can be applied to targeted detection of the polarity of cell lysosomes, and has a good application prospect.

The invention belongs to the field of biomedicine, and discloses a method and a kit for monitoring a lysosome acidification process mediated by mitochondrial-lysosome interaction. According to the method, an LYSO-PZ probe with a specific structure is used for marking cell lysosome contents, a pH rodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined for monitoring the pH value and membrane positioning of the lysosome respectively, then a three-channel fluorescence image is obtained through a structural illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of forming mitochondrial-lysosome contact (MLC) through optogenetics regulation, related protein binding is induced through blue light, fluorescence intensity changes before and after MLC formation are compared, and MLC-mediated proton transport is verified. A complete monitoring system is established for the first time, lysosome acidification can be accurately monitored, information can be obtained in multiple dimensions, and the method has important clinical application value and wide market prospects.

The application belongs to the field of biomedicine, and discloses a method and a kit for monitoring a process of mitochondrion-lysosome interaction-mediated lysosome acidification. The method is characterized in that a cell lysosome content is first labeled with a LYSO-PZ probe with a specific structure, a pHrodo dye and a lysosome membrane marker sfGFP-LAMP1-mCherry are combined to monitor the pH value and membrane localization of the lysosome, respectively, then three-channel fluorescence images are acquired through a structured illumination microscope (SIM), and the fluorescence intensity ratio is quantitatively analyzed. In addition, the method further comprises a step of optogenetic regulation of mitochondrion-lysosome contact (MLC) formation, the fluorescence intensity change before and after the formation of MLC is compared through blue light induction of related protein binding, and the proton transport mediated by MLC is verified. The application first establishes a complete monitoring system, can accurately monitor lysosome acidification, and has important clinical application value and broad market prospect.

The application discloses a kind of targeting lysosome, pH activated SO2 fluorescent probe and its preparation and application.The probe presents good cell lysosome targeting ability, and under the action of acidic environment in lysosome, can release sulfur dioxide and derivative reaction site, SO2 And derivative can carry out Michael addition with unsaturated carbon-carbon double bond released by molecule, change the conjugated structure of molecule, cause the change of probe color and fluorescence, with strong fluorescence intensity, can realize the imaging and rapid detection of SO2 And derivative in lysosome, show good practical applicability.The preparation method of the probe of the application is simple, raw material is diversified, easy to mass production and application.

The invention discloses application of a triple hydrocarbon activation strategy to synthesis of novel fluorescent amino acid, belonging to the technical field of organic synthesis. The method comprises the following steps: by taking phenylalanine derivatives and diaryl acetylene as starting substrates, reacting for 12 hours at the temperature of 120 DEG C under the action of a rhodium catalyst to obtain a series of five-fused-ring fluorescent amino acids. The method has the advantages that raw materials are easy to prepare, only one reaction step is needed, and experimental operation is simple; fluorescence experiments show that the obtained fluorescent amino acid has good fluorescence, and the maximum emission wavelength is as high as 628nm; a cell co-localization experiment shows that the five-ring-fused fluorescent amino acid has a good targeting effect on cell lysosome. # imgabs0 #

The invention discloses a lysosome targeting fluorescent probe compound based on a hydrogen peroxide response group protection mechanism. The lysosome targeting fluorescent probe compound comprises a naphthalimide fluorophore, a morpholine group and an N-methyl iminodiacetic acid borate (MIDA borate) protection structure. The morpholine group endows the lysosome with targeting ability, the MIDA borate improves the blood circulation stability, and the naphthalimide fluorophore generates a fluorescence signal after responding to H2O2. The synthesis method comprises the steps of synthesis of a naphthalimide precursor, introduction of a morpholine group and protection of MIDA borate. The probe can specifically recognize H2O2, the lowest detection limit reaches 0.25 mu M, the probe can target HeLa cell lysosome and realize high-sensitivity detection, and the cytotoxicity is low. Response group protection and targeting functions are creatively combined, the problems that an existing probe is poor in blood circulation stability and insufficient in detection specificity are solved, and the probe has important practical value in the fields of disease mechanism research, early diagnosis, medicine research and development and the like.

The invention relates to a preparation method and application of a BODIPY-based J aggregate capable of specifically inducing cell PD-L1 protein degradation. A basic building unit of the J aggregate is a conjugate of a BODIPY dye mother nucleus and a PD-L1 inhibitor BMS-37 derivative, and a J aggregate nano solution stably dispersed in a water system can be obtained under the assistance of a small amount of F127. The unique J-shaped dye arrangement enables BMS structural units to be fully displayed on the surface of an aggregate, so that the aggregate can be more combined with tumor cells, efficient degradation of PD-L1 is realized by virtue of a cytolysosome endocytosis way, the difficulty of a traditional ICB therapy is effectively broken through, hypoxia-tolerant near-infrared phototherapy performance is also obtained, and the application prospect is broad. The tumor immunotherapy effect is expected to be greatly improved. According to the BODIPY-based J aggregate membrane protein degradation strategy provided by the invention, ICB and ICD therapies are fully fused, various protein inhibitors and BDPEt-Az can be coupled through click chemistry reaction, more tumor immunotherapy reagents can be developed, and the BODIPY-based J aggregate membrane protein degradation strategy has a wide application prospect in the field of tumor immunotherapy.

The invention provides an aggregation-induced emission (AIE) fluorescent probe (Z)-3-[4-(bis {4-[2-morpholinyl ethyoxyl] phenyl} amino) phenyl]-2-(4-quinolyl) acrylonitrile for targeted lysosome detection of HSO3 <->, namely a fluorescent probe MoTAQ as well as a preparation method and application thereof in the aspect of detection. The weak alkalinity of the morpholine ring group in the probe and the weak acidity in the cell lysosome attract each other to realize lysosome targeting; according to the fluorescent probe, a molecular structure is modified, so that the molecular structure can realize specific detection of HSO3 <->, and meanwhile, the wavelength of a fluorophore in the fluorescent probe is shifted from red light to blue light before and after HSO3 <-> detection, so that effective observation can be performed through color change during fluorescence detection; secondly, MoTAQ has good fluorescence emission spectrum characteristics, and the fluorescent probe has high signal-to-noise ratio in vivo and in vitro; in addition, the fluorescent probe disclosed by the invention also has good biocompatibility.

The present invention discloses a fluorescent probe, a preparation method, and its biological application. The fluorescent probe of the present invention has a simple synthesis method, inexpensive raw materials, and is easily reproducible. It can achieve real-time imaging of lysosomes in living cells, has high specificity, is localized in lysosomes, is insensitive to pH and polarity, has strong resistance to photobleaching, high biocompatibility, and low cytotoxicity. The present invention relates to the field of material synthesis and biological imaging analysis technology. The fluorescent probe, preparation method, and its biological application have lysosomal targeting specificity, are insensitive to pH and polarity, have strong resistance to photobleaching, high biocompatibility, and are essentially non-toxic to cells. Therefore, the fluorescent probe of the present invention is particularly suitable for real-time imaging of lysosomes in living cells.