53 results about "Cytolysosome" patented technology

The invention discloses a cytolysosome-targeting anticancer cluster and a preparation method thereof. The anticancer cluster is prepared by mixing a small-molecular anticancer drug serving as a ligand with chloroauric acid and radiating a formed mixture with gamma rays. The prepared anticancer cluster is an 8e-structure anti-cancer nanocluster with precise atoms; the number of anti-cancer drugs in the nanocluster is precise, introduction of external lysosome targeting groups is not needed, the anti-cancer drugs can naturally penetrate through cell membranes to be enriched in lysosome, precise chemotherapy drug delivery is conducted, and cancer cells are effectively killed; and therefore, other diseases caused by excessive chemotherapeutic drugs in the body are avoided, and the nano-cluster directly synthesized by the anti-cancer drugs has an application value in realizing targeted quantitative administration and cancer treatment.

The invention relates to a nano gel containing platinum nano particles and a preparation method and application thereof. The nano gel takes cisplatin as a cross-linking agent in advance, can wrap quinacrine dye, and then reduces the cisplatin in the nano-gel in situ under the reduction of ethanol so as to form the platinum nano particles. Quinacrine is a lysosome dye, and can label lysosomes in cells in combination with the action of the nano gel. By utilizing the superoxide dismutase-like characteristics of the platinum nano particles, in the fluorescence imaging process, the platinum nano particles can remove active oxygen free radicals generated by illumination induction, so that the lysosomes can be protected from being damaged in the imaging process, and the long-term, stable and low-phototoxicity cell lysosome imaging is realized.

The invention belongs to the technical field of preparation of antitumor drugs, and specifically discloses a polypeptide micellar diagnostic reagent AGPR with aggregation-induced emission (AIE) properties and its application in near-infrared region biological imaging. The diagnostic reagent AGPR is composed of amphiphilic small molecule functional peptide ACBT-GGFLG-PEG n -R 8 GD, 4≤n≤20, spherical peptide micelles formed by self-assembly in aqueous solution. The novel AIEgen compound ACBT designed and synthesized by the present invention is a linear D-A-A molecule, and its emission peak is located in the near-infrared region. The present invention further uses the multifunctional chimeric peptide GGFLG-PEG 8 -R 8 GD modified the ACBT molecule to achieve high-efficiency enrichment of AIEgen molecule (ACBT) in tumor sites, and significantly enhanced fluorescence intensity in Cathepsin B-rich cell lysosomes / endosomes, with excellent imaging effects, so it is expected Applied to specific tracking of tumor cells.

The invention discloses a two-photon pH (potential of hydrogen) ratio measurement fluorescence probe for monitoring cell autophagy, a preparation method of the fluorescence probe and an application. The structure of the two-photon pH (potential of hydrogen) ratio measurement fluorescence probe for monitoring cell autophagy is as shown in the specification. The fluorescence probe has specific fluorescence signal response to pH. By the aid of a cell co-localization test, the fluorescence probe can be specifically targeted to cell lysosomes, a cell toxicity test indicates that the fluorescence probe has almost no toxic and side effect on cells, a two-photon confocal fluorescence micro-imaging test indicates that the fluorescence probe has good penetrability for MCF-7 cells, pKa of the fluorescence probe is 3.88 through calculation, the fluorescence probe is suitable for monitoring change ranges of the pH of the cell lysosomes, and the cell autophagy process can be monitored in real time by detecting change of the pH of the cell lysosomes.

The invention provides a rhodamine-sialic acid conjugate and a synthesizing method and application thereof to lysosome imaging, and relates to the rhodamine-sialic acid conjugate. In the rhodamine-sialic acid conjugate, a connecting arm connected between rhodamine and sialic acid adopts an amido bond or other structures. The rhodamine-sialic acid conjugate is prepared through the steps of adding 9-amino sialic acid and N-hydroxysuccinimide rhodamine X-5 carboxylic acid ester to a mixed solution of dioxane and water, adjusting pH to 9 through a saturated sodium carbonate solution, performing areaction for a night, removing a solvent of a reaction system, and performing HPLC separation to obtain the rhodamine-sialic acid conjugate. Living cell lysosomes are accurately positioned, and the signal-noise ratio is high; red fluorescence is launched in the lysosomes, the acidity of the lysosomes is changed, and a probe maintains hyperfluorescence; retention in the lysosomes is independent ofthe acidity of the lysosomes; real-time fluorescence imaging is performed on the lysosomes; and the rhodamine-sialic acid conjugate disclosed by the invention is used for the lysosomes to perform random restructured fluorescence microscopic imaging of ultrahigh resolution.

The invention relates to a pyrrole-rhodamine acylhydrazone derivative as well as a preparation method and application of the derivative, belonging to the organic synthesis field. The preparation method of the pyrrole-rhodamine acylhydrazone derivative comprises the following steps: (S1) adding N-(2-morpholin-4-ethyl)-5-formyl-2,4-dimethyl-1H-pyrrole-3-carboxamide into an organic solvent, and adding rhodamine 6G hydrazide, so as to obtain a mixture; (S2) carrying out reflux stirring reaction on the mixture obtained in the step (S1) at a normal pressure; (S3) after the reaction is finished, cooling to the room temperature so as to separate solids, carrying out reduced pressure filtration, and extracting filter residue; and (S4) washing the filter residue obtained in the step (S3), so as to obtain the pyrrole-rhodamine acylhydrazone derivative. The pyrrole-rhodamine acylhydrazone derivative is capable of selectively acting with bivalent copper ions, blue fluorescence is changed into greenfluorescence, a ratio fluorescence effect is realized, the naked eye distinguishing detection can be realized, and particularly, the pyrrole-rhodamine acylhydrazone derivative can be taken as a fluorescence probe to be applied to the convenient detection of bivalent copper ions in cell lyase.