3 results about "Cytolysosome" patented technology

The application discloses a pinacine alkyl tetrahydrocarbazole fluorescent probe capable of detecting cell lysosome polarity and a preparation method and application thereof. 9-(3-(dimethylamino)propyl)-3,3-dimethyl-2,3,4,9-tetrahydro-1H-2,4-bridged methylene carbazole-6-formaldehyde is used as raw material, and condensation reaction is carried out with 1,3-indan dione to obtain compound 2-(2-(9-(3-(dimethylamino)propyl)-3,3-dimethyl-2,3,4,9-tetrahydro-1H-2,4-bridged methylene carbazole-6-yl)vinyl)-1-indene-1,3(2H)-dione (TC-AP-ID), and the probe can specifically detect the polarity of cell lysosomes. In a mixed system of 1,4-dioxane and water, with the increase of the polarity of the system, the fluorescence color of the probe gradually changes from orange to red, and the fluorescence intensity significantly decreases. The probe has the advantages of simple synthesis, good selectivity, high sensitivity, good biocompatibility, low toxicity and the like, can be applied to targeted detection of the polarity of cell lysosomes, and has a good application prospect.

The invention discloses a lysosome targeting fluorescent probe compound based on a hydrogen peroxide response group protection mechanism. The lysosome targeting fluorescent probe compound comprises a naphthalimide fluorophore, a morpholine group and an N-methyl iminodiacetic acid borate (MIDA borate) protection structure. The morpholine group endows the lysosome with targeting ability, the MIDA borate improves the blood circulation stability, and the naphthalimide fluorophore generates a fluorescence signal after responding to H2O2. The synthesis method comprises the steps of synthesis of a naphthalimide precursor, introduction of a morpholine group and protection of MIDA borate. The probe can specifically recognize H2O2, the lowest detection limit reaches 0.25 mu M, the probe can target HeLa cell lysosome and realize high-sensitivity detection, and the cytotoxicity is low. Response group protection and targeting functions are creatively combined, the problems that an existing probe is poor in blood circulation stability and insufficient in detection specificity are solved, and the probe has important practical value in the fields of disease mechanism research, early diagnosis, medicine research and development and the like.

The invention provides an aggregation-induced emission (AIE) fluorescent probe (Z)-3-[4-(bis {4-[2-morpholinyl ethyoxyl] phenyl} amino) phenyl]-2-(4-quinolyl) acrylonitrile for targeted lysosome detection of HSO3 <->, namely a fluorescent probe MoTAQ as well as a preparation method and application thereof in the aspect of detection. The weak alkalinity of the morpholine ring group in the probe and the weak acidity in the cell lysosome attract each other to realize lysosome targeting; according to the fluorescent probe, a molecular structure is modified, so that the molecular structure can realize specific detection of HSO3 <->, and meanwhile, the wavelength of a fluorophore in the fluorescent probe is shifted from red light to blue light before and after HSO3 <-> detection, so that effective observation can be performed through color change during fluorescence detection; secondly, MoTAQ has good fluorescence emission spectrum characteristics, and the fluorescent probe has high signal-to-noise ratio in vivo and in vitro; in addition, the fluorescent probe disclosed by the invention also has good biocompatibility.