Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Aggregation-induced-emission polypeptide-micelle-type diagnostic reagent and application thereof in near-infrared-region biological imaging

A technology of aggregation-induced luminescence and diagnostic reagents, which can be applied to preparations, peptides, and drug delivery for in vivo experiments. It can solve the problems of lack of tumor targeting, reduced biological safety, and complex synthesis process, and achieve biodegradability. Diversified properties and functions, high yield, and easy synthesis

Active Publication Date: 2021-09-24
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
View PDF5 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the synthesis process of AIEgen molecules reacting with polymers is complicated, and it is easy to introduce organic reagents, which reduces its biological safety, and the molecules themselves lack tumor targeting, so long-term high-efficiency and low-toxicity tracers cannot be carried out.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Aggregation-induced-emission polypeptide-micelle-type diagnostic reagent and application thereof in near-infrared-region biological imaging
  • Aggregation-induced-emission polypeptide-micelle-type diagnostic reagent and application thereof in near-infrared-region biological imaging
  • Aggregation-induced-emission polypeptide-micelle-type diagnostic reagent and application thereof in near-infrared-region biological imaging

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] The synthesis of embodiment one, ACBT

[0058] Synthesize ACBT according to the following synthetic route:

[0059]

[0060] The synthesis steps of ACBT are:

[0061] 7-bromobenzo[c][1,2,5]thiadiazole-4-carbaldehyde (abbreviated as ABB) (1mmol), 4-triphenylamine borate (2mmol), anhydrous sodium carbonate (1.06g, 10mmol ), tetrakis(triphenylphosphine)palladium (50mg, 0.04mmol) was put into a three-necked flask, and nitrogen gas was fed to remove the oxygen in the flask. Then, 20 mL of toluene and 3 mL of water were injected into the flask under a nitrogen atmosphere, and the flask was placed in an oil bath at 110° C. for reflux reaction for 24 h. After the reaction, 40 mL of dichloromethane and 4.0 g of silica gel powder were added, and spun into powder using a rotary evaporator. Compound 1 (abbreviated as ABT) was purified by silica gel column chromatography, using a mixed solvent of dichloromethane and petroleum ether with a volume ratio of 3:1 as the eluent, and...

Embodiment 2

[0064] Example 2. Synthesis of aggregation-induced luminescent polypeptide micellar diagnostic reagent AGPR

[0065] 1. The functional chimeric peptide GGFLG-PEG was synthesized by the standard Fmoc solid-phase peptide synthesis method 8 -R 8GD: Weigh 2-chloro-trityl chloride resin (0.8g, 0.97mmol / g) into the peptide solid-phase synthesis column, add 20 mL N,N-dimethylformamide (DMF) to soak the resin for 30 minutes, Make it fully swell and remove DMF by suction filtration. Prepare to insert the first amino acid and enter the amino acid condensation reaction. Then, a DMF solution in which Fmoc-Asp(OtBu)-OH (3 times the degree of substitution of the resin) and DIEA (6 equivalents of the degree of substitution of the resin) was dissolved was added to the polypeptide solid-phase synthesis column, and reacted for 2 hours. After the reaction, the filtrate was sucked away and washed 4 times with DMF. The peptide resin after condensation of the first amino acid protected by FMOC ...

Embodiment 3

[0069] Example 3. Self-assembly performance and morphology characterization of aggregation-induced luminescent polypeptide micellar diagnostic reagent AGPR

[0070] Using pyrene as a hydrophobic fluorescent probe, the critical micelle concentration (CMC) value of AGPR was measured by fluorescence spectrophotometer to evaluate the self-assembly performance and stability of AGPR. Accurately weigh 9.7 mg of hydrophobic pyrene fluorescent molecule, dissolve it in 10 mL of acetone, and then dilute to 4.6 × 10 -6 mol / L, spare. Into 3.6mL AGPR aqueous solution (take the ACBT-GGFLG-PEG prepared in step 2 of Example 2) that has prepared a series of concentrations 8 -R 8 GD powder was dissolved in deionized water) Add 45μL 4.6×10 -6 mol / L pyrene solution in acetone, configured as a sample. Place the prepared sample in a shaker (150r / min) at 37°C for 24 hours to ensure that the acetone is completely volatilized. Finally, the emission spectrum of the sample solution within 360-400 n...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of preparation of antitumor drugs and particularly discloses a polypeptide-micelle-type diagnostic reagent AGPR with an aggregation-induced emission (AIE) property and application of the polypeptide-micelle-type diagnostic reagent AGPR in near-infrared-region biological imaging. The diagnostic reagent AGPR is of a spherical polypeptide micelle formed by self-assembly of an amphiphilic micromolecular functional peptide ACBT-GGFLG-PEGn-R8GD in an aqueous solution, wherein n is greater than or equal to 4 and less than or equal to 20. The novel AIEgen compound ACBT designed and synthesized by the invention is a linear D-A-A molecule, an emission peak value of the novel AIEgen compound ACBT is located in a near-infrared region I; and the ACBT molecule is further modified by using a multifunctional chimeric peptide GGFLG-PEG8-R8GD, so that the AIEgen molecule (ACBT) can be efficiently enriched in a tumor region, and the novel AIEgen compound ACBT has obviously enhanced fluorescence intensity in a cytolysosome / endosome rich in Cathepsin B, has an excellent imaging effect, and therefore can be advantageously applied to specific tracing of tumor cells.

Description

technical field [0001] The invention belongs to the technical field of anti-tumor drug preparation, and specifically relates to a polypeptide micelle-type diagnostic reagent AGPR with aggregation-induced emission (AIE) properties and its application in near-infrared region biological imaging. Background technique [0002] For a long time, fluorescent materials have provided important application value in the fields of biological imaging, molecular recognition and tracing, environmental detection and protection, and medical diagnosis. Existing fluorescent tracer reagents (such as fluorescent proteins, quantum dots, luciferase and organic fluorescent molecular dyes, etc.) have many problems, among which luciferase and fluorescent proteins need gene transfection, resulting in the normal physiological function of cells will be affected. Interference; quantum dots are toxic to heavy metals, and their fluorescence intensity will drop sharply during cell differentiation; traditiona...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08G01N21/64A61K49/00
CPCC07K7/08G01N21/6402A61K49/0021A61K49/0056A61K49/0054A61K49/0082
Inventor 程崟家秦四勇张爱清
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com