Nano gel containing platinum nano particles and preparation method and application thereof

A platinum nanoparticle and nanogel technology, applied in the field of drug carrier preparation, can solve the problems of phototoxic lysosome rupture, non-specificity, and easy photobleaching, etc.

Active Publication Date: 2019-09-13
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

LysoTracker is a commercial lysosome dye widely used in laboratories, but one of its disadvantages is that it is prone to photobleaching, making it unsuitable for long-term lysosome imaging
In the case of high concentration of acridine orange dye, the staining is non-specific, and the long-term fluorescence observation leads to the generation of phototoxicity and the rupture of lysosomes
Qu is also one of the commonly used lysosomal dyes, which has the characteristics of high photostability, but has certain cytotoxicity and obvious phototoxicity.

Method used

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  • Nano gel containing platinum nano particles and preparation method and application thereof
  • Nano gel containing platinum nano particles and preparation method and application thereof
  • Nano gel containing platinum nano particles and preparation method and application thereof

Examples

Experimental program
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Embodiment 1

[0066] Preparation of mother liquor: Weigh sodium hyaluronate (molecular weight 10kDa to 1000kDa), cisplatin, and adipine, respectively add double distilled water to make an aqueous solution (heating to aid dissolution, if necessary); the concentrations are:

[0067] -Concentration of hyaluronic acid aqueous solution: 1mg / ml to 5mg / ml;

[0068] - Cisplatin aqueous solution concentration: 3mg / ml to 20mg / ml;

[0069] - Concentration of Adipine solution in water: 1 mg / ml to 10 mg / ml.

preparation example 1

[0070] Preparation Example 1. Preparation of Nanogel Containing Platinum Nanoparticles ( figure 1 )

[0071] (1) Add 150 μL of the above cisplatin aqueous solution and 50 μL of double distilled water, add 800 μL of the above hyaluronic acid aqueous solution and mix quickly;

[0072] (2) heating the mixture at 90°C for 0.5 hours to 3 hours;

[0073] (3) Add 250 μL of 30%-100% (v / v) ethanol as a reducing agent, and continue to incubate at 90° C. for 2 hours to 8 hours;

[0074] (4) cooling on ice for 5 minutes to 15 minutes;

[0075] (5) The mixture is transferred to the dialysis bag for dialysis, and the dialysate is double distilled water;

[0076] (6) Store the obtained product at 4°C for future use.

preparation example 2

[0077] Preparation Example 2. Preparation of Nanogel Containing Functional Molecules and Platinum Nanoparticles ( Figure 4 )

[0078] (1) Add 150 μL of the above cisplatin aqueous solution and 50 μL of adipine aqueous solution to 800 μL of hyaluronic acid aqueous solution and mix quickly;

[0079] (2) heating the mixture at 90°C for 0.5 hours to 3 hours;

[0080] (3) Add 250 μL of 30%-100% (v / v) ethanol as a reducing agent, and continue to incubate at 90° C. for 2 hours to 8 hours;

[0081] (4) cooling on ice for 5 minutes to 15 minutes;

[0082] (5) The mixture is transferred to the dialysis bag for dialysis, and the dialysate is double distilled water;

[0083] (6) Store the obtained product at 4°C for future use.

[0084] It should be understood that the atipine in the step (1) is a representative functional molecule, and other dyes or drugs can be used. The reaction volumes described can be scaled up.

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Abstract

The invention relates to a nano gel containing platinum nano particles and a preparation method and application thereof. The nano gel takes cisplatin as a cross-linking agent in advance, can wrap quinacrine dye, and then reduces the cisplatin in the nano-gel in situ under the reduction of ethanol so as to form the platinum nano particles. Quinacrine is a lysosome dye, and can label lysosomes in cells in combination with the action of the nano gel. By utilizing the superoxide dismutase-like characteristics of the platinum nano particles, in the fluorescence imaging process, the platinum nano particles can remove active oxygen free radicals generated by illumination induction, so that the lysosomes can be protected from being damaged in the imaging process, and the long-term, stable and low-phototoxicity cell lysosome imaging is realized.

Description

technical field [0001] The invention relates to the technical field of drug carrier preparation, in particular to a hyaluronic acid nanogel containing platinum nanoparticles and fluorescent dyes, its preparation method and its application in lysosome labeling. Background technique [0002] Phototoxicity refers to the generation of reactive oxygen species (ROS) by fluorescent molecules in the process of fluorescent imaging of biological samples under the excitation of light and the presence of free oxygen, thereby inducing cytotoxicity. The photochemical principle of phototoxicity has been widely used in photodynamic therapy, but the phototoxicity produced by this process greatly limits the application of biological imaging. Since both fluorescent molecules and photoexcitation are involved in bioluminescent imaging, phototoxicity is unavoidable. [0003] In the process of biological imaging, it is usually used to reduce the time of excitation light irradiation in the fluores...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/36A61K33/243A61P35/00G01N21/64
CPCA61K9/06A61K47/36A61P35/00A61K33/243G01N21/6428
Inventor 张卫奇许海燕
Owner THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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