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Copper-terephthalic acid nano particle, and preparation method and applications thereof

A technology of terephthalic acid and nanoparticles, applied in the field of nano-medical device development, can solve the problems of low accuracy, fluorescence quenching, misdiagnosis, etc., and achieve the effect of accurate labeling

Active Publication Date: 2019-08-20
HUANGHE S & T COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, H 2 o 2 Widely distributed in cells, using H 2 o 2 Sensitive probe to label cellular lysosomes suffers from strong background interference
These methods relying on a single factor to label cell lysosomes are less accurate and often cause misdiagnosis in clinical applications
In addition, the fluorescence of currently commercialized lysosome-labeled probes is easily quenched by external factors. These probes need to be protected from light when used, which brings a lot of inconvenience to practical applications.

Method used

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  • Copper-terephthalic acid nano particle, and preparation method and applications thereof
  • Copper-terephthalic acid nano particle, and preparation method and applications thereof
  • Copper-terephthalic acid nano particle, and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of copper-terephthalic acid nanoparticles, the specific process is as follows:

[0036] To prepare uniform copper-terephthalic acid nanoparticles, take 0.05 mmol copper acetate (Cu(CH 3 COO) 2 ) (0.009083g) was dispersed in 20 mL of 0.08% by mass soluble starch (Sinopharm Chemical Reagent Co., Ltd.) aqueous solution, and terephthalic acid (C 8 h 6 o 4 ) (0.005815 g, 0.035 mmol) and sodium hydroxide (NaOH) (0.0028 g, 0.07 mmol) were dispersed in 10 mL of distilled water, and then the solution was added dropwise to the above mixed solution of copper acetate and starch, and stirred for 10 min. Then the solution was left to stand for 24 h, and finally centrifuged and washed to obtain the product.

[0037] The scanning electron microscope images of the above products are as follows: figure 2 shown by figure 2 It can be seen that the product has good dispersion, and the shape of a single particle is similar to a pinecone, with a long axis of about...

Embodiment 2

[0039] Detection of Decomposition Ability of Copper-terephthalic Acid Nanoparticles in Acidic Environment

[0040] Disperse the sample prepared as described in Example 1 in the acetic acid-sodium acetate buffer solution with pH 7 and 4 (the concentration of the sample in the buffer is 12.5 μg / mL), and stir at 37 °C for 4 h , after the product was centrifuged, the supernatant was taken, detected by UV-Vis, and the Cu in the solution was qualitatively determined according to the intensity of the absorption peak at 750 nm. 2+ Content changes, see the results for details Figure 4 .

[0041] Depend on Figure 4 It can be seen that under acidic conditions, copper-terephthalic acid nanoparticles in the supernatant obviously belong to Cu 2+ Absorption peak, while there is no obvious Cu in the supernatant under neutral conditions 2+ The absorption peak of , indicating that the nanoparticles are unstable under acidic conditions, easy to decompose and release Cu 2+ , and is stable ...

Embodiment 3

[0043] Zeta potential detection of copper-terephthalic acid nanoparticles in different pH environments

[0044] The sample prepared as described in Example 1 was dispersed in the acetic acid-sodium acetate buffer solution with a pH of 7 and 4 (the concentration of the sample in the buffer was 12.5 μg / mL), and the concentration of the nanoparticles was detected by a Zata potentiometer. Zeta potential under different pH conditions, the results are detailed in Figure 5 .

[0045] Depend on Figure 5 It can be seen that the Zeta potential of nanoparticles is relatively stable and does not change with the pH of the environment.

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Abstract

The invention provides a copper-terephthalic acid nano particle, and a preparation method and applications thereof. The copper-terephthalic acid nano particle can release Cu<2+> under an acidic condition and can be oxidized by hydroxyl free radicals to generate fluorescent substances. Soluble starch is arranged in the copper-terephthalic acid nano particle and is capable of maintaining a stable Zeta potential of the copper-terephthalic acid nano particle in solutions with different pH values. The nano particle is used as a nano fluorescence imaging probe and can be decomposed in acidic lysosome to release Cu<2+>; and Cu<2+> can further catalyze decomposition of H2O2 in lysosome of cells to generate hydroxyl free radicals. Hydroxyl free radicals can oxidize terephthalic acid of the nano fluorescence imaging probe to obtain hydroxyl terephthalic acid with a fluorescent property, thus the fluorescence imaging strength of the lysosome of cells is obviously higher than the fluorescence imaging strength of other parts of cells, and labeling of lysosome is realized therefore.

Description

technical field [0001] The invention belongs to the field of nanomedical device development, and in particular relates to a copper-terephthalic acid nanoparticle, its preparation method and application. Background technique [0002] The abnormal behavior of cell lysosomes is closely related to many diseases in the human body. Effective labeling and tracking of lysosomes is very important for disease diagnosis. The pH value in the lysosome (pH 4.5~5.5) is generally lower than the pH value of the cytoplasmic matrix (pH~7.4) (Analytical Chemistry, Vol. 87, 1499-1502). Based on this characteristic, the pH-sensitive probe prepared to label The work of cellular lysosomes has been extensively reported (Analytica Chimica Acta, Vol. 988, 66-73). However, the pH of endosomes is also acidic, a factor that severely interferes with the accuracy of pH-sensitive probes for lysosome labeling. Lysosome function and intracellular H 2 o 2 content, and compared with the cytoplasmic matrix,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00G01N21/64
CPCC08G83/008G01N21/6428G01N2021/6439
Inventor 陈健王是杰张莹
Owner HUANGHE S & T COLLEGE
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