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Method for targeted detection of H2S in lysoome through carbon quantum dots

A technology of carbon quantum dots and lysosomes, which is applied in the field of biosensors and ion recognition, can solve the problems of high cytotoxicity and poor biocompatibility, and achieve the effects of good biocompatibility, low cytotoxicity and cheap raw materials

Inactive Publication Date: 2017-06-20
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of poor biocompatibility and high cytotoxicity of existing fluorescent probes, and provide a carbon quantum dot targeted detection of H in lysosome 2 The method of S, which creatively proposes carbon quantum dots with good biocompatibility and low cytotoxicity as fluorescent probes for the detection of hydrogen sulfide in cell lysosomes, in order to realize the clinical detection of H in cell lysosomes 2 S offers the possibility

Method used

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  • Method for targeted detection of H2S in lysoome through carbon quantum dots
  • Method for targeted detection of H2S in lysoome through carbon quantum dots
  • Method for targeted detection of H2S in lysoome through carbon quantum dots

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Embodiment 1

[0032] Preparation method of carbon quantum dots C-dots

[0033] Weigh 0.9g of o-phenylenediamine, add it into 90mL of ethanol and stir until completely dissolved, then quickly transfer the above solution to a polytetrafluoroethylene-lined autoclave, then place it in an oven at 180°C for 12 hours, and cool naturally to room temperature, remove. The reacted mixed solution was concentrated under reduced pressure to obtain a black powder crude product. The crude product was subjected to silica gel column chromatography using dichloromethane and methanol at a volume ratio of 50:1 as the eluent to obtain a yellow-green product, which was finally obtained by rotary evaporation under reduced pressure and vacuum drying under reduced pressure to obtain a yellow powder as C-dots.

[0034] Preparation method of carbon quantum dot CD-dopa-morph fluorescent probe

[0035] Add 30mL of methanol and 10mL of dichloromethane into the container, and bubble with nitrogen for 2h to remove the ox...

Embodiment 2

[0036] Example 2, H 2 S selectivity experiment

[0037] The carbon quantum dot CDs-dopa-morph was added to the glycine buffer solution of pH=5.0 to prepare a solution of known concentration, and the stock solutions prepared by different interfering substances were added to it respectively, and the fluorescence intensity was tested. Interfering substances contain: K + , Fe 3+ , Zn 2+ , Mg 2+ 、Na 2 SO 3 , NaHSO 3 , NO 2 - , NO 3 - , AA, GSH all substance concentration 200μM, H 2 The concentration of S was 10 μM. It can be clearly seen from the fluorescence spectrum that only the H 2 The sample solution of S has a strong fluorescence enhancement for the probe at 575nm, showing a strong response to H 2 The efficient selectivity of S (such as Figure 5 shown), while samples containing other common ions, amino acids, etc. showed very weak fluorescence changes. Thus, it can be judged that the prepared CDs-dopa-morph fluorescent probe of the present invention is 2 S h...

Embodiment 3

[0038] Embodiment 3, the preparation of sensor solution

[0039] The carbon quantum dot CDs-dopa-morph is added to the glycine buffer solution with pH=5.0 to prepare a solution with known concentration. With the configured solution can be used for H 2 Qualitative and quantitative detection of S, such as Figure 6-Figure 8 shown. From Figure 8 Quantitative analysis data can be obtained in the present invention carbon quantum dot CDs-dopa-morph is used for H 2 Standard equation for quantitative detection of S.

[0040] Below in conjunction with example further describe the present invention to H 2 Quantitative identification detection of S.

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Abstract

The invention relates to a method for targeted detection of H2S in lysoome through carbon quantum dots, and belongs to the technical field of a biosensor and ion recognition. The fluorescence intensity of a synthesized carbon quantum dot CD-dopa-morph fluorescent probe and H2S in a 575 nm position can be obviously enhanced through photoinduced electron transfer. The H2S detection lowest limit of the carbon quantum dot fluorescent probe reaches 70nM. The carbon quantum dot fluorescent probe has the advantages that the synthesis method is simple; the biocompatibility is good; the cell toxicity is low; the detection sensitivity is high; the detection is fast; the signal is stable; the possibility is provided for the realization of clinic detection of H2S in cytolysosome.

Description

technical field [0001] The invention relates to a carbon quantum dot targeted detection of H in lysosomes 2 The method of S belongs to the field of biosensor and ion recognition. Background technique [0002] In recent years, hydrogen sulfide (H 2 S) is widely present in mammals including humans, especially in lysosomes of cells, and plays a very important biological role. Hydrogen sulfide has been considered as the third gas signal molecule after nitric oxide (NO) and carbon monoxide (CO), which has a variety of physiological functions, including regulating blood pressure, alleviating ischemia-reperfusion injury, and exerting anti-inflammatory effects. Lower metabolic rate etc. Changes in hydrogen sulfide levels are associated with various diseases, such as Down syndrome, Alzheimer's disease, etc. [0003] There are many detection technologies for hydrogen sulfide, such as: polarographic technology, electrode technology, chromatographic technology, etc. Although the abo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 束庆海吴小雪李丽洁陈树森牛虎赵帅赵思维
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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