Gold nanoparticle flower or quantum dot composite probe for living cell immunofluorescent labeling and photothermal treatment

A technology of gold nanoflowers and composite probes, which is used in nanotechnology, fluorescence/phosphorescence, nanotechnology, etc. for materials and surface science, and can solve the problem of difficult control of composite probes, large absorption spectrum width, and difficulty in controlling consistency etc. to achieve high photothermal conversion efficiency, enhanced fluorescence intensity, and avoidance of biological toxicity

Inactive Publication Date: 2015-03-18
THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is easy to cause the fluorescence of quantum dots to weaken
The patent number is 201310117034.4, and the patent title is "a gold nanostar quantum dot composite cell probe with dual functions of photothermal and fluorescence enhancement and its preparation method and application". Although the disclosed composite probe has photothermal conversion and However, due to the following three reasons, some improvements are needed to make it more suitable for photothermal-fluorescent composite labeling in vivo: (1) due to the small number of gold nanostar spikes, The length of each spike is not easy to control the consistency, resulting in a large width of its absorption spectrum, which overlaps with the absorption spectrum of near-infrared quantum dots. In this way, some photothermal effects will also be generated when quantum dots are excited to emit light (that is, fluorescent tracer). The brightness of near-infrared light has a certain impact (but it has an enhancement effect on light with a wavelength shorter than orange, so it is suitable for marking superficial tumors); (2) The photothermal threshold of gold nanostars is 1.8W / cm 2 There is still room for further reduction; (3) Due to the longer spikes of gold nanostars, the 2 1. When preparing composite probes, it is not easy to control the diameter of composite probes to be less than 100nm, so it has a certain degree of negative impact on the labeling of living cells

Method used

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  • Gold nanoparticle flower or quantum dot composite probe for living cell immunofluorescent labeling and photothermal treatment
  • Gold nanoparticle flower or quantum dot composite probe for living cell immunofluorescent labeling and photothermal treatment
  • Gold nanoparticle flower or quantum dot composite probe for living cell immunofluorescent labeling and photothermal treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Add 0.6mL of 10mM NaBH in ice bath 4 Add to 10mL containing 0.85mM HAuCl 4 and 0.1 M CTAB in water, the solution was aged at 29 °C for at least 3 hours. Spawns small golden balls.

[0053] (2) 6 mL of 0.5 mM HAuCl 4 The solution, 6mL of 0.2M CTAC and 4.5mL of 0.1M AA were mixed and 0.3mL of the seeds prepared in (1) were added. Centrifuge and dissolve in 1mL water. Get larger golden balls.

[0054] (3) Mix 5.8mL DMF (including 0.43mM PVP (MW=55000)) and 40uL HAuCl 4 (24.28mM) into the vial, then add 200uL of the seed crystal in step (2), and stir at 80 degrees Celsius for 1 hour to obtain the seed crystal for growing gold nanoflowers. The shape and size of the seed crystal are as follows Figure 8 (a) shown.

[0055] (4) 15mL 9M PVP in DMF solution, add 150uL HAuCl 4 , 2400uL of the seed crystal prepared in step (3) was added and stirred for a few hours until the reaction was complete. The generated gold nanoflowers were centrifuged and dissolved in alcohol ...

Embodiment 2

[0060] (1) Add 0.6mL of 10mM NaBH in ice bath 4 Add to 10mL containing 1.5mM HAuCl 4 and 0.1 M CTAB in water, the solution was aged at 29 °C for at least 3 hours. Spawns small golden balls.

[0061] (2) 6 mL of 0.5 mM HAuCl 4 The solution, 6mL of 0.2M CTAC and 4.5mL of 0.1M AA were mixed and 0.3mL of the seeds prepared in (1) were added. Centrifuge and dissolve in 1mL water. Get larger golden balls.

[0062] (3) Mix 5.8mL DMF (including 0.43mM PVP (MW=55000)) and 40uL HAuCl 4 (24.28mM) was added to the vial, followed by 200uL of the seeds from (2) and stirred at 80°C for 1 hour. A seed crystal for growing gold nanoflowers is obtained. The shape and size of the seed crystal are as Figure 8 (b) shown.

[0063] (4) 15mL 9M PVP in DMF solution, add 180uL HAuCl 4 , 2400uL (3) The prepared seed crystals were added and stirred for a few hours until the reaction was complete. The generated gold nanoflowers were centrifuged and dissolved in alcohol solution. The excitation ...

Embodiment 3

[0067] (1) Add 0.6mL of 10mM NaBH in ice bath 4 Add to 10mL containing 3mM HAuCl 4 and 0.1 M CTAB in water, the solution was aged at 29 °C for at least 3 hours. Spawns small golden balls.

[0068] (2) 6 mL of 0.5 mM HAuCl 4 The solution, 6mL of 0.2M CTAC and 4.5mL of 0.1M AA were mixed and 0.3mL of the seeds prepared in (1) were added. Centrifuge and dissolve in 1mL water. Get larger golden balls.

[0069] (3) Mix 5.8mL DMF (including 0.43mM PVP (MW=55000)) and 40uL HAuCl 4 (24.28mM) was added to the vial, followed by 200uL of the seeds from (2) and stirred at 80°C for 1 hour. A seed crystal for growing gold nanoflowers is obtained. The shape and size of the seed crystal are as Figure 8 (c) shown.

[0070] (4) 15mL 9M PVP in DMF solution, add 180uL HAuCl 4 , 1920uL seed crystals were added and stirred for a few hours until the reaction was complete. The generated gold nanoflowers were centrifuged and dissolved in alcohol solution. The excitation wavelength that pro...

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Abstract

The present invention provides are a gold nanoflower structure and a preparation method therefor. The gold nanoflower structure is a gold nanoflower particle, with round-head columns being uniformly distributed at the periphery thereof, obtained by using gold octahedrons, gold balls or gold tetrahedrons as seed crystals and reducing chloroauric acid by using weak reductant in an environment of high-concentration polyvinylpyrrolidone. In addition, also provided in the present invention are a gold nanoflower/quantum dot composite probe for living cell immunofluorescent labeling and photothermal therapy, a preparation method therefor and a use thereof. In comparison with traditional probes, the probe, incorporates the features of photothermal therapy and fluorescent labeling, and is capable of killing cancer cells in an effective and directional way. Two light sources are adopted to bring a tremendous photothermal conversion efficiency and a greater enhancement on fluorescence intensity of quantum dots respectively, thus mutual interference of two effects are avoided tactfully. The coating of silicon dioxide averts the biotoxicity of the gold nanoflower and the quantum dot effectually, enabling the surface of the composite probe to be easily functionalized and also imparting an extraordinarily excellent biocompatibility to the composite probe.

Description

technical field [0001] The invention relates to the technical field of composite nanoprobes, in particular to a gold nanoflower / quantum dot composite probe for immunofluorescence labeling and photothermal treatment of living cells. Background technique [0002] Quantum dots are nanomaterials artificially synthesized by a small number of atoms, also known as "artificial atoms". After being excited, they can emit fluorescence, change the structure of quantum dots, and adjust their excitation and emission wavelengths at will. It is used to label and locate the target cells after the specific antibody is activated, and maintain its physicochemical and luminescence stability in the cells for a long time. Replacing traditional dyes with quantum dots as fluorescent markers can overcome the disadvantages of large dye molecules (thus needing to make holes in cells and cannot be used for in vivo labeling), poor photochemical stability and spectral characteristics, and can be used for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F1/00B22F1/02B22F9/24C09K11/02C12Q1/02G01N21/64A61K41/00A61K9/14A61P35/00B82Y30/00B82Y40/00B22F1/054B22F1/16
CPCG01N21/6428C09K11/88B22F9/24A61K41/0052A61K47/6929A61P35/00B22F1/0553B22F1/054B22F1/16B22F1/056
Inventor 蒋童童朱立新许小亮
Owner THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
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