4 results about "Immunofluorescent labeling" patented technology

The application discloses a plastic embedding agent BMA / IBOMA resin, monomers of the resin are composed of butyl methacrylate (BMA) and isobornyl methacrylate (IBOMA) in a mass ratio of 6:1 to 7:2. The application further discloses a preparation method of the plastic embedding agent, which comprises the steps of mixing the two resins according to the mass percentage, and then adding a crosslinking agent and an initiator. The application further discloses a plastic embedding method compatible with multi-color fluorescence and immunofluorescence labeling, which adopts the BMA / IBOMA resin to embed a biological tissue sample. The embedding method has low cost, and the BMA and IBOMA are used as hydrophobic resin monomers to effectively maintain the fluorescence signal of the sample. In addition, the IBOMA can improve the cutting performance of the sample after embedding, and meet the 1-micron precision cutting requirement of the fMOST system.

The embodiment of the invention provides a blood leukocyte precise separation method combining immune density and a centrifugation technology. The blood leukocyte precise separation method comprises the following steps: performing multicolor immunofluorescence labeling on leukocyte subgroups in a blood sample; performing flow cytometry analysis on the marked sample, and establishing an initial multicolor fluorescence compensation matrix for the current sample individual; dynamically adjusting and generating a corrected compensation matrix of the individual specificity based on the deviation degree of the initial compensation matrix in a sample group reference range; carrying out association mapping on a specific physical sorting interval which is determined after the signal recalculation and the enclosure and is rich in the target leukocyte subgroup and a density gradient centrifugal separation condition; and directly carrying out density gradient centrifugation operation on the blood sample subjected to immunolabeling by utilizing the association mapping. According to the scheme of the embodiment of the invention, the high-purity specific leukocyte subgroup can be efficiently separated from a complex blood sample on the premise of keeping high cell activity and flux.

This invention discloses a detection method for cellular senescence based on the synergistic detection of SA-β-Gal and γH2AX dual biomarkers, belonging to the field of biotechnology. This invention first performs SA-β-Gal staining on live cells, then fixes the cells for γH2AX immunofluorescence labeling, achieving "dual biomarker detection in the same cell," saving time and reducing sample loss. Through the dual determination of "γH2AX positive (indicating DNA damage) and SA-β-Gal positive (indicating functional arrest)," this invention significantly improves the specificity and accuracy of senescent cell identification, reducing the false positive or false negative rate of single biomarkers. It greatly avoids the problems of "missing early senescence" or "misjudging damaged repair cells" associated with single biomarkers, enabling dynamic tracking of the aging process. Compared to traditional detection methods, this invention uses a non-invasive method, enabling sensitive, efficient, and rapid quantitative and qualitative analysis of non-fixed senescent cells, breaking through the bottlenecks of traditional detection methods, achieving high specificity, high accuracy, and high compatibility, and can be widely applied in clinical diagnosis.

The invention relates to the technical field of biomedical engineering, and discloses a method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, and the method comprises the following steps: S1, respectively separating microbial communities from root systems of healthy soybean plants and susceptible soybean plants to obtain a healthy group microbial suspension and a susceptible group microbial suspension; according to the method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, phytophthora sojae and associated microorganisms are specifically recognized through an immunofluorescence labeling technology, fluorescence-negative flora in healthy soybean root systems is precisely enriched in combination with flow cytometry sorting, screening leakage of non-culturable microorganisms is avoided from the source, and the yield of root rot resistance is improved. Meanwhile, the invalid workload in the screening process is greatly reduced, the mining period of the core beneficial endophytic flora is shortened, and the pertinence and efficiency of the screening process are improved.