8 results about "Immunofluorescent labeling" patented technology

The application discloses a plastic embedding agent BMA / IBOMA resin, monomers of the resin are composed of butyl methacrylate (BMA) and isobornyl methacrylate (IBOMA) in a mass ratio of 6:1 to 7:2. The application further discloses a preparation method of the plastic embedding agent, which comprises the steps of mixing the two resins according to the mass percentage, and then adding a crosslinking agent and an initiator. The application further discloses a plastic embedding method compatible with multi-color fluorescence and immunofluorescence labeling, which adopts the BMA / IBOMA resin to embed a biological tissue sample. The embedding method has low cost, and the BMA and IBOMA are used as hydrophobic resin monomers to effectively maintain the fluorescence signal of the sample. In addition, the IBOMA can improve the cutting performance of the sample after embedding, and meet the 1-micron precision cutting requirement of the fMOST system.

The disclosure provides methods for detecting circular endothelial cells (CECs) in a non-enriched blood sample. The present disclosure is based, in part, on the unexpected discovery that CECs can be detected in non-enriched blood samples. The present disclosure is further based, in part, on the discovery that CECs can be detected in non-enriched blood samples by combining the detection of one or more immunofluorescent markers in the nucleated cells of a non-enriched blood sample with an assessment of the morphology of the nucleated cells. The present disclosure is further based, in part, on the discovery that CECs can be detected in non-enriched blood samples by comparing the immunofluorescent marker staining and morphological characteristics of CECs with the immunofluorescent marker staining and morphological characteristics of WBCs. The methods disclosed herein serve to classify human subject in myocardial infarction (MI) patients or healthy controls.

The embodiment of the invention provides a blood leukocyte precise separation method combining immune density and a centrifugation technology. The blood leukocyte precise separation method comprises the following steps: performing multicolor immunofluorescence labeling on leukocyte subgroups in a blood sample; performing flow cytometry analysis on the marked sample, and establishing an initial multicolor fluorescence compensation matrix for the current sample individual; dynamically adjusting and generating a corrected compensation matrix of the individual specificity based on the deviation degree of the initial compensation matrix in a sample group reference range; carrying out association mapping on a specific physical sorting interval which is determined after the signal recalculation and the enclosure and is rich in the target leukocyte subgroup and a density gradient centrifugal separation condition; and directly carrying out density gradient centrifugation operation on the blood sample subjected to immunolabeling by utilizing the association mapping. According to the scheme of the embodiment of the invention, the high-purity specific leukocyte subgroup can be efficiently separated from a complex blood sample on the premise of keeping high cell activity and flux.

The application provides application of a compound shown in formula (III) in multicolor immunofluorescence staining. The method can be used for marking a plurality of different fluorescent dyes on a plurality of antigens in a sample, and then the fluorescent dyes in the last round of staining can be eluted through an elution process, so that a new round of multicolor immunofluorescence marking can be started. The marked sample can be used for imaging by instruments and equipment such as a fluorescence microscope or a laser confocal microscope, and after software synthesis, the sample marked by a plurality of different colors can be displayed. The colors are clear, bright and do not bleed. The application not only retains the sensitivity and staining intensity of the TSA fluorescent staining method, but also overcomes the limitation of the number of multicolor fluorescent markers.

This invention discloses a detection method for cellular senescence based on the synergistic detection of SA-β-Gal and γH2AX dual biomarkers, belonging to the field of biotechnology. This invention first performs SA-β-Gal staining on live cells, then fixes the cells for γH2AX immunofluorescence labeling, achieving "dual biomarker detection in the same cell," saving time and reducing sample loss. Through the dual determination of "γH2AX positive (indicating DNA damage) and SA-β-Gal positive (indicating functional arrest)," this invention significantly improves the specificity and accuracy of senescent cell identification, reducing the false positive or false negative rate of single biomarkers. It greatly avoids the problems of "missing early senescence" or "misjudging damaged repair cells" associated with single biomarkers, enabling dynamic tracking of the aging process. Compared to traditional detection methods, this invention uses a non-invasive method, enabling sensitive, efficient, and rapid quantitative and qualitative analysis of non-fixed senescent cells, breaking through the bottlenecks of traditional detection methods, achieving high specificity, high accuracy, and high compatibility, and can be widely applied in clinical diagnosis.

The invention relates to the technical field of biomedical engineering, and discloses a method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, and the method comprises the following steps: S1, respectively separating microbial communities from root systems of healthy soybean plants and susceptible soybean plants to obtain a healthy group microbial suspension and a susceptible group microbial suspension; according to the method for promoting root rot resistance by analyzing soybean core endophyte flora based on fluorescence labeling, phytophthora sojae and associated microorganisms are specifically recognized through an immunofluorescence labeling technology, fluorescence-negative flora in healthy soybean root systems is precisely enriched in combination with flow cytometry sorting, screening leakage of non-culturable microorganisms is avoided from the source, and the yield of root rot resistance is improved. Meanwhile, the invalid workload in the screening process is greatly reduced, the mining period of the core beneficial endophytic flora is shortened, and the pertinence and efficiency of the screening process are improved.

The invention discloses a marker of human umbilical cord mesenchymal stem cells and an immunofluorescence labeling detection method thereof, and relates to the technical field of cells, the marker of the human umbilical cord mesenchymal stem cells hUC-MSCs is Runx1t1, the umbilical cord mesenchymal stem cells are cultured in vitro, and Runx1t1 fluorescence labeling is positive.

The application discloses a kind of based on super-resolution image's platelet microtubule classification method and classification system, the classification method, including the following steps: obtaining immunofluorescence labeled platelet microtubule super-resolution image;With single platelet microtubule structure distribution as classification object, according to classification principle is divided into "regular distribution", "diffuse distribution", "aggregated distribution" and "irregular distribution" four categories, the classification method can be used to classify platelet microtubule in sample, and it has important significance to platelet physiological function research.In addition, based on the classification method, the platelet microtubule classification system based on super-resolution image provided by the application can automatically extract the complete image of single platelet microtubule based on the obtained platelet microtubule super-resolution image, and mark the type of microtubule according to the classification principle, and automatically display the classification result, which can automatically classify platelet microtubule based on platelet microtubule super-resolution image.