Immunofluorescence method for locating woody plant pollen tube protein and kit thereof

Abstract

The invention provides an immunofluorescence method for locating a woody plant pollen tube protein and a kit thereof. The kit includes a fixing solution, an enzymolysis solution, a blocking solution,a primary antibody and a secondary antibody; the fixing solution contains PEPES, sucrose, Triton X-100, paraformaldehyde, NaCl, KCl, Na2HPO4 and KH2PO4, the enzymolysis solution contains cellulase, macerozyme, NaCl, KCl, Na2HPO4 and KH2PO4, the blocking solution contains bovine serum albumin, NaCl, KCl, Na2HPO4 and KH2PO4, the primary antibody is a target protein antibody, and the secondary antibody is a FITC-labeled goat anti-rabbit secondary antibody. The method adopts an apple pollen tube as a test material, after enzymatic hydrolysis of the wall of the apple pollen tube, immunofluorescentlabeling is performed. The method can directly, efficiently, sensitively and specifically locate the functional protein in the pollen tube, and provides a technical system for visually identifying theexpression of the functional protein in the pollen tube of a woody plant and locating the functional protein in the pollen tube of the woody plant.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows researchers to easily find specific types or parts (polypetide) within wood plant cells that are important functions like photosynthesis. It involves creating an organic mixture containing sugars from sugar beets by cutting off their shells with scissors. These mixtures then reacted with certain chemical agents under controlled conditions such as temperature, pH value, etc., allowing them to bind these molecules together into larger structures called polymer chains. By measuring changes caused when some components have been added during this process, scientists may study how different genotypes affect genetic activity.

Problems solved by technology

This patented describes an approach called transfusional flow cytometry or Western blotting, where specific DNA sequences found within certain types of vegetative material like roots may help determine how well they function properly during various stages of life's history. These techniques require specialized equipment with advanced tools and knowledge about root structure and biological processes. There has been some progress towards understanding this problem because these methods involve cutting off parts of the plant's outer layer - also known as cuff-wall structures – making them hard to analyze further.

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