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An immunochromatographic detection method based on near-infrared luminescent rare earth nanomaterials

A rare earth nanomaterial, immunochromatographic detection technology, applied in material inspection products, analytical materials, measuring devices, etc., can solve the problems of limited luminescence brightness of materials, low luminescence quantum efficiency, influence of immunochromatography sensitivity, etc., to achieve repeatability Strong, strong fluorescence emission, good stability

Active Publication Date: 2021-01-26
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the luminescence quantum efficiency of existing near-infrared luminescent rare earth nanomaterials is very low, usually lower than 1%, and the luminescence brightness of the material is very limited, which will have a great impact on the sensitivity of immunochromatographic detection.

Method used

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  • An immunochromatographic detection method based on near-infrared luminescent rare earth nanomaterials
  • An immunochromatographic detection method based on near-infrared luminescent rare earth nanomaterials
  • An immunochromatographic detection method based on near-infrared luminescent rare earth nanomaterials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: NaYF 4 :Yb,Nd@NaYF 4 Preparation and Luminescent Properties of Near Infrared Rare Earth Nanomaterials

[0070] (1) Synthesis of NaYF by solvothermal method 4 :Yb,Nd@NaYF 4

[0071] Prepare inner core NaYF 4 :Yb,Nd:

[0072] Weigh 1 mmol of rare earth acetate Ln(CH 3 COO) 3 (wherein yttrium acetate Y(CH 3 COO) 3 Accounting for 33%, ytterbium acetate Yb (CH 3 COO) 3 Accounting for 7%, Nd(CH 3 COO) 3 Accounting for 60%) into a 100mL three-necked flask, add 6mL oleic acid and 15mL 1-octadecene; heat up to 120°C under a nitrogen atmosphere, and stir until the solid is completely dissolved.

[0073] Heating was then stopped, the solution was cooled to room temperature, and a solution containing 2.5 mmol NaOH and 4 mmol NH was added 4 The methanol solution of F was 10 mL; under nitrogen purging, the solution was heated to 120°C to remove the methanol in the solution; finally, the solution was heated to 300°C and maintained for 1 h.

[0074] After the ...

Embodiment 2

[0083] Example 2: LiYF 4 : Preparation and Luminescent Properties of Nd Near Infrared Rare Earth Luminescent Nanomaterials

[0084] (1)LiYF 4 : Synthesis of Nd

[0085] Weigh 0.95mmolYCl 3 , 0.05molNdCl 3 , 6 mL of oleic acid, and 15 mL of 1-octadecene were put into a 100 mL three-necked flask; vacuum was applied to remove oxygen and water in the system, and the temperature was raised to 130°C until the solid was completely dissolved and the solution was clear and transparent.

[0086] Then the solution was cooled to room temperature, and the solution containing 0.106g LiOH and 0.148gNH was added dropwise 4 F methanol solution 8mL. Nitrogen was blown, and the solution was heated to 120°C to remove methanol. After 30 minutes, the reaction system was sealed, and the temperature was rapidly raised to 300°C, and maintained for 90 minutes. After the reaction is completed, the system is cooled to room temperature, and the precipitate obtained by centrifuging the solution is Li...

Embodiment 3

[0091] Example 3: NaYbF 4 :Er,Ce@NaYF 4 :Yb@NaYbF 4 :Nd@CaF 2 Preparation and luminescent properties of near-infrared luminescent nanomaterials

[0092] (1)NaYbF 4 :Er,Ce@NaYF 4 :Yb@NaYbF 4 :Nd@CaF 2 Synthesis

[0093] Weigh 1mmol rare earth trifluoroacetate Ln(CF 3 COO) 3 (where Yb(CF 3 COO) 3 Accounting for 93%, Er(CF 3 COO) 3 Accounted for 2%, Ce(CF 3 COO) 3 Accounting for 5%) into a 100mL three-neck flask, add 10mmol oleic acid, 10mmol oleylamine and 20mmol 1-octadecene; raise the temperature to 120°C under a nitrogen atmosphere, and stir until the solid is completely dissolved. Heating was then stopped, the solution was cooled to room temperature, and a solution containing 2.5 mmol NaOH and 4 mmol NH was added 4 The methanol solution of F was 10 mL; under nitrogen purging, the temperature of the solution was raised to 120°C to remove the methanol in the solution; finally, the temperature of the solution was raised to 300°C and maintained for 30 minutes. A...

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Abstract

The invention discloses an immunochromatographic detection method based on a near-infrared luminescent rare earth nano-material, which uses the near-infrared luminescent rare earth nano-material as animmunofluorescent marker to mark and recognize antibodies for performing immunochromatographic detection. The near-infrared luminescent rare earth nano-material has the following characteristics: (1)both the excitation light and the emission light are located in the near-infrared light region from 700 to 1700 nm; (2) the excitation wavelength is less than the emission wavelength, and the excitation light is a positive Stokes shift luminescence, and the Stokes shift is greater than 150 nm; (3) the fluorescence quantum efficiency is higher than 1%; (4) the fluorescence lifetime is greater than10 microseconds; and (5) the light stability is better than organic dyes. By using the immunochromatographic detection method based on the near-infrared luminescent rare earth nano-material disclosedby the invention for immunochromatographic detection, the stability of the fluorescent marker is better, the fluorescence emission is stronger, and the detection result has better repeatability.

Description

technical field [0001] The invention belongs to the technical field of immunochromatographic detection, and in particular relates to an immunochromatographic detection method based on near-infrared luminescent rare earth nanomaterials. Background technique [0002] Biological samples such as blood absorb and scatter near-infrared light much weaker than visible light, and biological samples have no obvious autofluorescence in the near-infrared light region. Using near-infrared as a detection signal can reduce background interference and signal attenuation. Therefore, near-infrared luminescent materials are gradually being applied to the field of immunochromatography. [0003] At present, most researchers choose near-infrared luminescent organic dyes for immunochromatography; however, the quantum efficiency of near-infrared organic dyes in aqueous solution is usually very low, and the optical stability is poor, which is mainly reflected in the phenomenon of photobleaching and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/533G01N33/68
CPCG01N33/533G01N33/68
Inventor 李富友刘青昀冯玮吴勇
Owner FUDAN UNIV
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