36 results about "High content imaging" patented technology

The invention relates to an evaluation method for measuring cell DNA damage caused by soluble heavy metal. The method includes the following steps that firstly, tested cells are processed through a soluble heavy metal solution; secondly, the processed tested cells are fixed and are subjected to cell permeabilization; thirdly, the tested cells having been subjected to cell permeabilization are dyed; fourthly, detection is performed through a high-content imaging system and analysis is performed. The evaluation method for measuring the cell DNA damage caused by the soluble heavy metal is convenient to operate, high in sensitivity and high in detection speed and has high specific pertinence, and research on the genetic toxicity of the heavy metal is supplemented and expanded.

The invention discloses application of a bifluorescence reporting system in tumor stem cell targeted drug screening and a method thereof. Red and green fluorescence proteins are utilized to respectively mark colony tumor cells and tumor stem cells, and the targeting properties of the drug for the tumor cells and tumor stem cells can be respectively evaluated by using a high-content imaging system. No targeted drug screening methods for tumor stem cells exist at present. The tumor stem cell screening method established by the invention is hopeful to be used for screening novel antineoplastic drugs for tumor stem cells, thereby enhancing the screening effect of the antineoplastic drugs.

A system and method that images biological samples and uses chromophores to analyze the imaged samples. The chromophore analysis can be done by itself or in conjunction with fluorophore analysis in High Content Imaging systems. To perform chromophore analysis the biological samples can be labeled with different chromophores and imaged using transmitted light that is at least partially absorbed by the chromophores. To also perform fluorophore analysis the samples can also be labeled with fluorophores that are excited by excitation light. The chromophore analysis and fluorophore analysis can be performed separately or concurrently using a High Content Imaging system. The system provides the expanded capability by illuminating the chromophore-labeled samples with transmitted light of different wavelengths and automatically detecting the images which represent the differential absorption of the colored lights by the sample.

The invention discloses a method for detecting the influence of a gum-based type tobacco product on the human oral cavity in vitro micronucleus. The method comprises: (1) placing a gum-based type tobacco product to be detected in liquid nitrogen, and freezing; (2) immediately crushing the frozen gum-based type tobacco product by using a crusher; (3) re-freezing; (4) soaking the crushed gum-based type tobacco product by using artificial saliva; (5) carrying out centrifugal separation on the extraction solution; (6) determining the supernatant through an osmotic pressure determining instrument, and adjusting the osmotic pressure to achieve the same osmotic pressure of a cell culture medium; (7) determining the nicotine content in the extraction solution; (8) culturing human oral mucosal epithelial cells as the detection object; (9) adding the extraction solution to the cultured human oral mucosal epithelial cells; and (10) after the incubation, detecting the cell in vitro micronucleus condition by using a high-content imaging instrument.

The invention discloses a method for quantitatively analyzing nicotine-induced cell DNA damage based on the high content technology. The method includes the steps of firstly, culturing cells; infecting the cells; thirdly, performing immunofluorescent labeling of gamma H2AX; fourthly, performing high-content-technology quantitative analysis. The method has the advantages that a high-content imaging system is used to perform automatic imaging, DNA double-strand break marker gamma H2AX protein generated by nicotine induction is quantitatively analyzed, direct and fast cell detection is achieved, and sample processing convenience is achieved; the high-resolution imaging allows the distribution of the gamma H2AX in each cell nucleus to be able to be observed directly and image data to be convenient to store and reanalyze, the nicotine-induced gamma H2AX in each cell nucleus can be quantitatively analyzed, and the detection result is sensitive and accurate.

The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and / or high content imaging. The compounds disclosed herein are novel reduced dyes, including Cy-based hydrocyanine dyes and Cy-based deuterocyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and / or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

The invention discloses a method for evaluating vascular toxicity of an aryl phosphate flame retardant based on a high-content imaging system. The method comprises the following steps: 1) acquiring azebra fish embryo / juvenile fish vascular fluorescence image treated by the aryl phosphate flame retardant; 2) performing blood vessel image segmentation identification and analysis; and 3) obtaining evaluation and screening results. Vascular images of transgenic zebra fish embryos / juvenile fishes marked by specific vascular fluorescence are collected, and polyvascular parameters are analyzed to realize evaluation and screening of the vascular development toxicity of the aryl phosphate flame retardants. The method comprises the steps that a hardware system is responsible for obtaining blood vessel image data and is composed of a high-content imaging system and a 96-hole glass bottom plate matched with agarose coating; and the software system is used for semi-automatic identification and multi-parameter output analysis of a target blood vessel, and relates to software such as Photoshop, Matlab and GraphPad Prism. The method provided by the invention has the characteristics of high flux and stable result, and can quickly and accurately evaluate and screen the vascular development toxicity of the aryl phosphate flame retardant with multiple parameter indexes.

The invention relates to an evaluation method for measuring cell DNA damage caused by soluble heavy metal. The method includes the following steps that firstly, tested cells are processed through a soluble heavy metal solution; secondly, the processed tested cells are fixed and are subjected to cell permeabilization; thirdly, the tested cells having been subjected to cell permeabilization are dyed; fourthly, detection is performed through a high-content imaging system and analysis is performed. The evaluation method for measuring the cell DNA damage caused by the soluble heavy metal is convenient to operate, high in sensitivity and high in detection speed and has high specific pertinence, and research on the genetic toxicity of the heavy metal is supplemented and expanded.

Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ROS) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and / or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ROS and measuring oxidative stress in cells either in vitro and / or in vivo. Also described herein are processes for preparing novel reduced dyes, i.e., ROS probes, for use in the disclosed compositions, methods and kits.

A system and method for segmenting an image captured from an image capture device of a high-content imaging system includes an image capture module that receives an image captured by the image capture device. A coarse object detection module generates a coarsely segmented image, wherein each pixel of the coarsely segmented image is associated with a corresponding pixel in the captured image and identified as one of an object pixel and a background pixel. The marker identification module selects at least one marker pixel from the pixels of the coarsely segmented image, wherein each marker pixel is an object pixel in a continuous group of object pixels in the coarsely segmented image, and the object pixel is relatively Neighboring pixels to the set of object pixels are farthest from background pixels. an object splitting module comprising a plurality of processors operating in parallel to associate each object pixel of a coarsely segmented image with a marker pixel, wherein, in the coarsely segmented image, where the object pixel is associated with the The distance-based measure between marker pixels is smaller than the distance-based measure between the object pixel and any other marker pixel.

The invention discloses a method for quantitatively detecting 1,3-butadiene-induced cell DNA damage in combination with a gas-liquid interface exposure system and a high-content technology. The method comprises the following steps: 1) cell culture; 2) 1,3-butadiene exposure through a gas-liquid interface exposure mode; 3) immunofluorescent labeling of gamma H2AX; 4) high-content detection. The method disclosed by the invention has the advantages that the efficient exposure of gaseous 1,3-butadiene to wall-attached cells in vivo is realized by adopting the in-vitro gas-liquid interface exposure mode, so that the exposure efficiency of the 1,3-butadiene is improved; a high-content imaging system is utilized for automatic imaging, 1,3-butadiene-induced DNA double-chain fracture maker gamma H2AX proteins are quantitatively analyzed, and the direct and quick detection of the cells is realized, so that the sample treatment is more convenient; through high-resolution imaging, not only can the distribution of the gamma H2AX in cell nucleuses be directly observed, but also image data is convenient to store and re-analyze, and the quantitative analysis on the 1,3-butadiene-induced gamma H2AX in each of the cell nucleuses can be realized, so that detection results are more sensitive and accurate.

The invention discloses a high-content super-resolution integrated microscopic imaging system and method. The system includes: a super-resolution microscopic imaging lighting module, a high-content imaging lighting module, a lighting optical path switching device, a microscopic imaging module, and an automatic scanning and imaging module. A focusing module; the illumination light path switching device is used to switch the super-resolution microscopic imaging illumination module or the high-content imaging illumination module into the system. The present invention has two working modes of high-content imaging and super-resolution microscopic imaging at the same time, and has the functions of intelligent cell identification and screening and rapid positioning, and the function of two-dimensional and three-dimensional super-resolution image reconstruction. The present invention conducts millimeter-level "macroscopic" image observation on the sample through a large field of view microscope objective lens, and the system performs precise partition coding on high-throughput images, intelligent cell screening, and automatic marking and positioning of regions of interest; then switches to super-resolution microscopy In the imaging mode, the super-resolution image is obtained by using structured illumination microscopic imaging technology.

The invention discloses an application of a diameter of aorta dorsalis of a juvenile zebrafish as an effect marker in detection of PCB126 cardiovascular toxicity, and a detection method. The diameterof aorta dorsalis of the juvenile zebrafish is obtained through subjecting a roe of the juvenile zebrafish to PCB126 contamination treatment within 2 hours of fertilization, fixing a posture after culturing the roe, performing image acquisition and carrying out measurement. The juvenile zebrafish is a transgenic juvenile zebrafish of which vascular endothelial cells are labeled with green fluorescence. The juvenile zebrafish is cultured in an illumination constant-temperature incubator with the temperature of 26.5 DEG C in a 14h / 10h light and dark cycle, and is cultured to 72 hpf. The image acquisition is performed by adopting a high-content imaging system for layer scanning to obtain a juvenile zebrafish blood vessel fluorogram. The measurement is carried out by measuring the diameter ofthe aorta dorsalis by using Photoshop software, corresponding original data is recorded by means of EXCEL, and then software GraphPad Prism is used for statistical analysis. The application is beneficial to characterizing cardiovascular toxicity caused by low-dose PCB126 exposure at an ambient level, and provides a technical method and a reference basis for environmental monitoring and evaluationas well as formulation of related technical standards.

The invention discloses a method for high-throughput screening of DNA damage response inhibitors, comprising the following steps: S1, training a cell nucleus segmentation network model based on a U-Net network; S2, constructing and training a cell nucleus category determination network model; S3. Use a high-content imaging device to photograph the cells subjected to the DNA damage response inhibitor to obtain an image to be analyzed; input the to-be-analyzed image into the cell nucleus segmentation network model, and then input the cell nucleus type determination network model, and count each DNA damage response inhibitor The corresponding proportion of damaged nuclei, the smaller the proportion of damaged nuclei, the better the effect of DNA damage response inhibitor. The method of the present invention can automatically and batchly perform segmentation and classification decisions for images obtained by high-content imaging equipment, and compounds with further research value can be preliminarily screened out through statistical analysis.

The invention discloses a method for evaluating the vascular toxicity of aryl phosphate flame retardants based on a high-content imaging system, which comprises the following steps: 1) obtaining vascular fluorescence images of zebrafish embryos / juveniles treated with aryl phosphate flame retardants ; 2) Segmentation, recognition and analysis of blood vessel images; 3) Obtaining evaluation and screening results. By collecting blood vessel images of transgenic zebrafish embryos / juveniles with specific blood vessel fluorescence labeling, and analyzing multiple blood vessel parameters, the evaluation and screening of vascular developmental toxicity of aryl phosphate flame retardants can be realized. The configuration of the method includes: the hardware system is responsible for the acquisition of blood vessel image data, which is composed of a high-content imaging system and a 96-well glass bottom plate coated with agarose; the software system is used for semi-automatic identification of target blood vessels and multi-parameter output analysis, involving Photoshop, Software such as Matlab and GraphPad Prism. The invention has the characteristics of high throughput and stable results, and can quickly and accurately evaluate and screen the vascular developmental toxicity of multi-parameter indexes for aryl phosphate flame retardants.

The invention discloses an in-vitro cell micronuclei method for detecting total particulate matters of main stream smoke of cigarettes. The method comprises the steps of seeding cells on a 96-hole plate for cigarette smoke contamination, directly fixing, dyeing, and feeding the cells into a high-connotation imaging system for detection; setting fluorescence intensity parameters of main nucleuses and the micronuclei of the cells according to the difference between a fluorescence value of cell nucleuses and a background fluorescence value, measuring the sizes of the main nucleuses and the micronuclei of the cells so as to set nucleus diameter parameters, and setting distance parameters according to distances between the cell nucleuses; performing analysis on images according to the setting, and selecting the number of binucleated cells with the micronuclei and the total number of the binucleated cells from a result lead-out column so as to calculate the micronucleus rate. According to the method, the number of required cells is small, the cells are not need to be used, and micronuclei sheets are not required to be manufactured; furthermore, the in-vitro cell micronuclei method is high in detection speed, large in sampling quantity and high in accuracy.