Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An evaluation method for the determination of cell DNA damage caused by soluble heavy metals

A DNA damage and evaluation method technology, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., to achieve the effect of easy repeatability, accurate and intuitive targets, fast and accurate simultaneous detection of multiple samples

Active Publication Date: 2017-06-27
CHINA TOBACCO YUNNAN IND
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the test using γH2AX index to evaluate DNA damage caused by heavy metals is rarely reported at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An evaluation method for the determination of cell DNA damage caused by soluble heavy metals
  • An evaluation method for the determination of cell DNA damage caused by soluble heavy metals
  • An evaluation method for the determination of cell DNA damage caused by soluble heavy metals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] The following are examples of the present invention, but the content of the present invention is not limited thereto.

[0030] 1. Experimental materials: Chinese hamster ovary cells (CHO cells) were provided by Kunming Institute of Zoology, Chinese Academy of Sciences.

[0031] 2. Main experimental equipment: high-content imaging system (ImageXpress MICRO, Molecular Devices); CO 2 Incubator (Thermo Company); secondary biological safety cabinet (Heal Force Company); inverted microscope (TS100-F-HMC type, Nikon Company); 96-well plate, cell culture flask (Corning Company, USA).

[0032] 3. Experimental method:

[0033] (1) Preparation of standard stock solution of hexavalent chromium: Weigh 2.9418g K 2 Cr 2 o 7 (Excellent grade pure), after dissolving in water, transfer to a 100ml volumetric flask, dilute to the marked line with water, and shake well. The solubility of this solution is 100mM, ready for use.

[0034](2) CHO cells were planted in a 96-well cell cultur...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an evaluation method for measuring cell DNA damage caused by soluble heavy metal. The method includes the following steps that firstly, tested cells are processed through a soluble heavy metal solution; secondly, the processed tested cells are fixed and are subjected to cell permeabilization; thirdly, the tested cells having been subjected to cell permeabilization are dyed; fourthly, detection is performed through a high-content imaging system and analysis is performed. The evaluation method for measuring the cell DNA damage caused by the soluble heavy metal is convenient to operate, high in sensitivity and high in detection speed and has high specific pertinence, and research on the genetic toxicity of the heavy metal is supplemented and expanded.

Description

technical field [0001] The invention relates to an evaluation method for measuring cellular DNA damage caused by soluble heavy metals by using a high-content imaging system, and belongs to the technical field of environmental ecotoxicology. Background technique [0002] Heavy metals exist in the air, soil, and water that humans depend on for survival, and there are various types, among which mercury, cadmium, lead, chromium, arsenic, etc. have significant biological toxicity, and can enter benthic organisms from water and soil through food chains and other channels , fish, birds and mammals, resulting in biomagnification. At present, heavy metal pollution exists in part of the soil and water in our country. Studies have shown that the toxicity of heavy metals has multi-faceted and multi-level characteristics. However, previous studies have mainly focused on the physical and chemical detection of pollutants. Ecological evaluation of heavy metals. Regarding the toxicity of h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 高茜夭建华黄海涛李雪梅米其利曾婉俐管莹朱洲海
Owner CHINA TOBACCO YUNNAN IND
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com