High-content super-resolution integrated microscopic imaging system and method

Abstract

The invention discloses a high-content super-resolution integrated microscopic imaging system and method. The system includes: a super-resolution microscopic imaging lighting module, a high-content imaging lighting module, a lighting optical path switching device, a microscopic imaging module, and an automatic scanning and imaging module. A focusing module; the illumination light path switching device is used to switch the super-resolution microscopic imaging illumination module or the high-content imaging illumination module into the system. The present invention has two working modes of high-content imaging and super-resolution microscopic imaging at the same time, and has the functions of intelligent cell identification and screening and rapid positioning, and the function of two-dimensional and three-dimensional super-resolution image reconstruction. The present invention conducts millimeter-level "macroscopic" image observation on the sample through a large field of view microscope objective lens, and the system performs precise partition coding on high-throughput images, intelligent cell screening, and automatic marking and positioning of regions of interest; then switches to super-resolution microscopy In the imaging mode, the super-resolution image is obtained by using structured illumination microscopic imaging technology.

Description

technical field [0001] The invention relates to the technical field of optical imaging, in particular to a high-content super-resolution integrated microscopic imaging system and method. Background technique [0002] The great progress of life science in various fields, especially the breakthrough achievements of cell biology, has revolutionized the position of life science in natural science. Since life science is an experimental subject, research in these cutting-edge directions relies on innovative technologies and equipment. High-content cell analyzers and super-resolution microscopic imaging analyzers are indispensable high-end equipment for current and future cell biology research. For the development of the most intuitive and clear imaging technology, researchers not only need to use high-content imaging technology to observe the macroscopic morphology of cells, but also need to use super-resolution microscopic imaging technology to obtain the images of living cells ...

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Problems solved by technology

[0004] To complete the above experimental process, it is necessary to transfer the cell samples to be tested from the high-content large-field-of-view device to the super-resolution small-field-of-view device. There are two natural defects in this process: 1) The live cell culture environment cannot be guaranteed to be consistent during the transfer process, resulting in cell Physiological state changes or even death; 2) Before and after the transfer, there is a lack of positioning benchmarks for the cells or regions of interest in the sample to be tested. The location of the cells of interest marked by the high-content equipment has expired after the transfer, and researchers can only re-find the cells of interest based on the approximate location. Cells, time-consuming and labor-intensive, can easily lead to experimental failure

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