System and method for automatically analyzing phenotypical responses of cells

Abstract

A system and a method to analyze a phenotypical response of cells to a treatment are disclosed in which a model development module receives images of a plurality of reference cell carriers and treatment information associated with the plurality of reference cell carriers, identifies parameters of cells in the image that distinguish those reference cell carriers to which the treatment has been applied from other reference cell carriers, and trains a model using the identified parameters. A high-content imaging system includes an image capture device, and the image acquisition module receives from the image capture device a plurality of images of cell carriers to be evaluated. The model application module applies the trained model to the plurality of images of the cell carriers to be evaluated to predict a concentration of the treatment applied to each of the cell carriers evaluated.

Description

[0001] related application [0002] This application claims the benefit of US Patent Application Serial No. 62 / 204,225, filed August 12, 2015, the contents of which are hereby incorporated by reference in their entirety. technical field [0003] The subject matter relates to high content imaging systems, and more specifically, to systems and methods for automated analysis of phenotypic responses of cells. Background technique [0004] Researchers can use high-content imaging systems (HCIS) to analyze the phenotypic response of biological cells to treatments, such as exposure to specific substances and / or other environmental changes. These responses may be visually distinct and may be observable in images acquired using fluorescent labeling and other techniques. [0005] However, this response can vary depending on the pathways involved in the response, cell tissue type, cell age, growth conditions, etc. The diversity of possible responses requires limiting the analysis of ...

AI Technical Summary

Problems solved by technology

The number of response types and the number of parameters that must be tuned in order to quantify each response can place a significant burden on researchers who must identify the phenotypic type of response that occurs and then manipulate the various parameters associated with that type. parameter to determine whether such a reaction did occur

[0006] Furthermore, in some cases it may not be practical or even possible to expose a homogenous population of target cells to the substance or environmental condition to be tested

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