Method for enriching Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water
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A technology of Giardia and Cryptosporidium is applied in the field of coagulation and enrichment of Cryptosporidium oocysts and Giardia cysts in water, which can solve the problems of low enrichment rate of the two worms, high operation requirements and high price. , to achieve the effect of reducing capital investment, improving enrichment rate and simple operation
Inactive Publication Date: 2011-09-21
INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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This method causes the loss of "two worms" in the process of eluting the filter capsule, which makes the enrichment rate of Cryptosporidium oocysts and Giardia cysts low. Studies have shown that Cryptosporidium oocysts and Giardia cysts The recovery rate is often only a low level of 20% to 30%.
This method has the problems of high operation requirements, expensive filtration equipment and consumables (such as special filter capsules, etc.), and low and unstable enrichment rate of the two worms.
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Embodiment 1
[0018] Using tap water as a water sample, polyaluminum chloride (PAC) and polyaluminum ferric chloride (PAFC) were used as coagulants to coagulate Cryptosporidium oocysts and Giardia cysts added to the water sample Enrichment. The sample volume of tap water is 1L, and Cryptosporidium oocysts and Giardia cysts are added respectively, so that the water contains about 1000 Cryptosporidium oocysts and Giardia cysts, and the pH value of the water sample is 9.0; The dosage is 30mg / L, the dosage of PAFC is 20mg / L, the stirring condition is rapid stirring at 180r / min for 2min, then slow stirring at 40r / min for 15min, and standing for 30min after the reaction.
[0019] Then discard the supernatant, collect the precipitated alum flowers, add 1 mL of 1.0 mol / L hydrochloric acid to the collected alum flowers, and stir to dissolve the alum flowers. The solution was centrifuged at 10,000 rpm for 10 minutes, then the supernatant was sucked out, and the sediment at the bottom of the centrifu...
Embodiment 2
[0024] Taking the water of Xinglinwan Reservoir in Xiamen City as a sample, using aluminum sulfate, ferric sulfate, ferric chloride, PAC, and PAFC as coagulants, the surrogate indicator (fluorescent Microspheres, divided into Φ6.0 μm for simulating Cryptosporidium oocysts and Φ10.0 μm for simulating Giardia lamblia cysts, were purchased from Polysciences, USA) for coagulation enrichment. The enrichment method is the same as in Example 1.
[0025] Table 2 shows the enrichment rate of Cryptosporidium microspheres and Giardia microspheres in surface water according to the present invention in Example 2.
[0026] Table 2 Enrichment rate of Cryptosporidium microspheres and Giardia microspheres added in surface water by different coagulants
[0027]
[0028] Visible by above embodiment, the present invention is to the enrichment rate of Cryptosporidium oocyst in tap water 73.8-92.9%, the enrichment rate of Giardia cyst is 73.8-78.6%, and Cryptosporidium microsphere and The high...
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Abstract
The invention relates to a method for enriching Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water. The method comprises the following steps: adding an inorganic metal salt coagulant into a bulk water sample, stirring, coagulating, precipitating, collecting precipitate alumen ustum in a small-volume container, adding acidic solution into the small-volume container to dissolve the alumen ustum, centrifuging, removing supernate and thus, enriching the Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water. In the invention, the problems of low Cryptosporidium parvum oocyst and Giardia Lamblia sporocyst enrichment rate, instability, high operation requirement and expensive filtering equipment and consumable items of the conventional method for enriching Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts. The method does not need expensive apparatus and equipment and medicines and greatly reduce capital investment. The method is simple in operation, easy to master, time-saving and labor-saving, reduces cost of enrichment of Cryptosporidium parvum oocysts and Giardia Lamblia sporocysts in water, improves enrichment efficiency and is a novel method which clears the preliminary work for subsequent steps of immunomagnetic separation, immunofluorescence label microscope counting, polymerase chain reaction (PCR) qualitative detection, nano probe gene chip detection and Real-time PCR quantitative detection and the like.
Description
technical field [0001] The invention relates to a method in the technical field of detection, in particular to a method for coagulating and enriching Cryptosporidium oocysts and Giardia cysts in water, and belongs to the technical field of water quality safety. Background technique [0002] Cryptosporidium parvum and Giardia lamblia are currently considered to be the two most important waterborne enteric pathogens. Cryptosporidium exists in the water in the form of oocysts (Oocyst), half of the infection dose is 132 live oocysts, and the pathogenic dose is generally 10 live oocysts; while Giardia exists in the form of cysts (Cyst) In water, the pathogenic dose is generally 10 to 25 living cysts. Drinking water containing "two worms" (Cryptosporidium parvum and Giardia lamblia) can cause digestive tract infections, accompanied by symptoms such as abdominal pain, diarrhea, and fever. The water-borne transmission of "two worms" will cause serious public safety incidents. At ...
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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24C12Q1/06G01N1/28
CPCY02A50/30
Inventor 于鑫张会宁古励魏博刘冰张舒婷邬卓颖许金丽
Owner INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI
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