The invention provides a cryptosporidium parvum immune colloidal gold detection test paper strip and a production method thereof. The test paper strip comprises a nitrocellulose membrane, a gold marking pad and an absorption pad, wherein the gold marking pad and the absorption pad are arranged at two ends of the nitrocellulose membrane; the upper end of the gold marking pad is a sample pad, and the gold marking pad is coated with a purified CSpV-S protein monoclonal antibody colloidal gold coupling marker, a detection line is coated with a purified monoclonal antibody, a quality control line is coated with a goat anti mouse IgG antibody, and the absorption pad is attached to the side of the quality control line. The research combines the enzyme-immunoassay principle and colloidal gold chromatography to prepare the colloidal gold test paper strip for detecting C.parvum, which is going to be applied to the clinic to improve the prevention and treatment ability of C.parvum, and the invention has the advantages of simple and rapid operation, clear detection results and easy judgment, high specificity, high sensitivity, no need of instruments and apparatuses or just need of simple instruments and the like, and therefore is especially suitable for clinic sample detection use in sites where diseases occur, outpatient departments, places having no experimental conditions and the like.