5 results about "Cryptosporidium parvum" patented technology

The application discloses application of a micro cryptosporidium cgd6_660 protein as an oocyst outer wall marker protein, and discloses that the inventors find that the cryptosporidium cgd6_660 protein is an oocyst outer wall protein, and the protein is located on the outer surface of the cryptosporidium oocyst wall. The inventors confirm the feasibility of the protein as a detection antigen. The antibody of the protein recombinant protein (as shown in a sequence table SEQ ID N0.2) can be used for immunological detection of unbroken cryptosporidium oocysts (live cryptosporidium oocysts), and the application further provides a specific polypeptide of the protein.

The application provides a double-PCR differential detection method for Cryptosporidium parvum and Eimeria bovis, which is used for synchronously differentiating and detecting Cryptosporidium parvum and Eimeria bovis in a to-be-detected sample; the double-PCR differential detection method uses the following primer combination: an upstream primer Cpar-F for Cryptosporidium parvum, a downstream primer Cpar-R for Cryptosporidium parvum, an upstream primer Ebov-F for Eimeria bovis, and a downstream primer Ebov-R for Eimeria bovis. The application also provides application of the double-PCR detection method and a kit for synchronously differentiating Cryptosporidium parvum and Eimeria bovis. The double-PCR differential detection method can simultaneously and specifically amplify two target fragments in the same reaction tube, so that rapid differentiation of two pathogens and mixed infection of the two pathogens can be realized.

The invention discloses a cryptosporidium parvum sporozoites activity detection method and device and application, and belongs to the technical field of biological detection and drug screening. The method comprises the following steps: providing a to-be-detected sample containing dead or live cryptosporidium parvum sporozoites; diluting the to-be-detected sample in a multiple ratio gradient manner and / or performing freeze thawing treatment, and adding the to-be-detected sample into a detection container; an ATP bioluminescence detection reagent is added into the detection container for oscillation treatment, so that cryptosporidium parvum sporozoite is split to release ATP, and a reaction is carried out to generate a luminescence signal; collecting the light-emitting signal, and quantitatively evaluating the survival rate or activity of the cryptosporidium parvum sporozoite according to the intensity of the light-emitting signal. The method provided by the invention is based on the ATP bioluminescence principle, can directly, quickly and quantitatively evaluate the activity of the sporozoite stage in a short time, and can be used for high-throughput screening of large-scale compound drug libraries.

The invention discloses a lactobacillus johnsonii YS my4 strain with a cryptosporidium infection resisting effect and application of the lactobacillus johnsonii YS my4 strain. The preservation number of the YS my4 strain is CCTCC (China Center For Type Culture Collection) M 20253011. The strain disclosed by the invention has the characteristics of cholate resistance, intestinal juice resistance, strong adhesiveness and capability of inhibiting salmonella and staphylococcus aureus. In addition, the strain has no hemolytic rings, and the strain has no influence on body indexes and can increase the body weight after gavage of suckling rats, so that the strain is proved to be high in safety and can be applied to the fields of medicine and food. According to the invention, the YS my4 strain is intragastrically fed to a pseudo-sterile suckling mouse infected by cryptosporidium parvum, and it is found that the strain can activate immune defense of the intestinal tract of a host by improving the MAPK phosphorylation level of the host in the state of cryptosporidium parvum infection, so that the insect loading amount of the cryptosporidium parvum is remarkably reduced; meanwhile, the YS my4 strain can improve the expression level of tight junction proteins Occludin and CLDN4, relieve pathological damage of ileum tissues and improve the intestinal barrier function, and has important application value in prevention and treatment of cryptosporidium parvum infection.

The application discloses a Cryptosporidium parvum CpDHR1 gene replacement strain and a construction method and application thereof. The application researches and finds that the CpDHR1 gene of Cryptosporidium parvum is related to virulence, the CpDHR1 gene of a high-virulence strain is replaced by the CpDHR1 gene of a low-virulence strain through a gene editing technology, and a Cryptosporidium parvum CpDHR1 gene replacement strain is successfully constructed. Research shows that after the CpDHR1 gene of a high-virulence wild-type Cryptosporidium parvum IIdA20G1 subtype strain is replaced, the strain can still be cultured in vitro, but the in-vitro growth of the strain is significantly reduced, the virulence of the strain is weakened, the oocyst excretion intensity of the strain is weakened, the oocyst load in mice is reduced, the clinical symptoms of the mice are alleviated, the survival time of the mice is prolonged, the virulence of the strain is significantly weakened, the strain can be used for preparing a Cryptosporidium parvum low-virulence vaccine product, and Cryptosporidium parvum infection can be better prevented.