Multiplex RT-PCR/PCR for simultaneous detection of bovine coronavirus, bovine rotavirus, Cryptosporidium parvum, and Escherichia coli

a multi-core, rtpcr technology, applied in the field of multi-core rtpcr/pcr method, can solve the problems of high cost, time-consuming and laborious process for detecting these pathogens, and currently used assays have severe limitations in detecting these agents, etc., to achieve rapid detection, sensitive, and specific
US20050239057A1Inactive Publication Date: 2005-10-27BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV
Publication Date
2005-10-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention provides a multiplex RT-PCR / PCR method, which enables in a single assay the simultaneous detection of any combination of bovine rotavirus, bovine coronavirus, Cryptosporidium parvum, and optionally, Escherichia coli strains producing K99 pili or heat-stable enterotoxin STa.
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Description

CROSS-REFERENCE TO RELATED APPLICATION

[0001] Not applicable. STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT

[0002] Not applicable. BACKGROUND OF THE INVENTION

[0003] (1) Field of the Invention

[0004] The present invention relates to a multiplex RT-PCR / PCR method, which enables in a single assay the simultaneous detection of any combination of bovine rotavirus, bovine coronavirus, Cryptosporidium parvum, and optionally, Escherichia coli strains producing K99 pili and heat-stable enterotoxin STa or STb.

[0005] (2) Description of Related Art

[0006] In the United States, bovine rotavirus, bovine coronavirus, Cryptosporidium parvum, and Escherichia coli strains producing K99 pili or fimbriae or heat-stable enterotoxin STa are the five most common infectious agents in neonatal calf diarrhea, commonly referred to as scours. The relative incidence of these pathogens in a multi-state survey of causes of scours in beef cattle was 35% for rotavirus and coronavirus combined, 2...

Claims

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