A fluorescence LAMP detection method for Cryptosporidium parvum
A technology of Cryptosporidium parvum and detection method, which is applied in the direction of microorganism-based method, measurement/inspection of microorganism, biochemical equipment and method, etc., which can solve the problem of low detection rate, high detection cost and difficult and other issues to achieve the effect of reducing the cost of detection
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[0027] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.
[0028] The cryptosporidium parvum fluorescence LAMP detection method comprises the following steps:
[0029] 1. First, according to the reference sequence of Cryptosporidium parvum DNAJ-like protein gene (AF177278) in GenBank, the reference sequence is as follows:
[0030]
[0031] 2. Specificity of different gene loci (SSU RNA, GP60, HSP70, actin) of Cryptosporidium parvum
[0032] Sex primer screening confirmed. Primer sequences are shown in Table 1:
[0033] Table 1 Specific primers for nested PCR detection of Cryptosporidium parvum
[0034]
[0035]
[0036] 3. According to the Cry...
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