30 results about "Giardia species" patented technology

Methods for aiding in the diagnosis of disorders including, but not limited to, PDDs (Pervasive Development Disorders), Dysautonomic disorders, Parkinson's disease and SIDS (Sudden Infant Death Syndrome). In one aspect, a diagnosis method comprises analyzing a stool sample of an individual for the presence of a biological marker (or marker compound) comprising one or more pathogens, which provides an indication of whether the individual has, or can develop, a disorder including, but not limited to, a PDD, Dysautonomia, Parkinsons disease and SIDS. Preferably, the presence of one or more pathogens is determined using a stool immunoassay to determine the presence of antigens in a stool sample, wherein such antigens are associated with one or more pathogens including, but not limited to, Giardia, Cryptosporidium, E. histolytica, C. difficile, Adenovirus, Rotavirus or H. pylori.

The invention relates to a filtration and concentration method for improving the detection recovery rate of cryptosporidium and giardia in the technical field of water environmental water quality monitoring. The method comprises the following steps: sampling and filtering; adding elution; ultrasound oscillation and centrifugal treatment; carrying out stained preparation by sequentially adopting an immunomagnetic separation method after completing the centrifugal treatment; and then counting by a fluorescence microscope, and making statistics for the recovery rate. The method improves certain operation steps in the filtration and concentration stages, so as to achieve the purposes of reducing the detection cost of the cryptosporidium and giardia and improving the recovery rate.

The invention discloses a method for detecting cryptosporidium by using a pyrosequencing technology. The method comprises the following steps of: firstly, designing specific primers and pyrosequencing primers according to cryptosporidium 18S rRNA (Ribosomal Robonucleic Acid); extracting desoxyribonucleic acid (DNA) of a sample to be tested; performing polymerase chain reaction (PCR) proliferation by using the specific primers of 18SrRNA genes; detecting a proliferated product by using agarose gel electrophoresis; preparing a pyrosequencing single-chain template if the proliferation segment length of the primer is 201bp; carrying out pyrosequencing reaction; and finally, determining whether the sample contains giardia according to a PCR proliferation result and a pyrosequencing result. The method is suitable for rapidly detecting and confirming the cryptosporidium, can be widely applied to water quality monitoring and control in an environment, oocyst detection of excrement of a human body and confirmation of protozoon in import and export trades, and is convenient to operate and small in required sample amounts.

The invention relates to an automatic quantitative tap water sampling device and method for detecting cryptosporidia and Giardia lamblia. With the method, samples of cryptosporidia and Giardia lamblia are automatically collected in the flowing process of tap water; since the quantities of cryptosporidia and Giardia lamblia in 100 L of water are needed in detection of cryptosporidia and Giardia lamblia, a tap water automatic quantifying controller is such configured that sampling is stopped when a water volume reaches 100 L; the samples are enriched in a cryptosporidium and Giardia lamblia detection and enrichment filter core; after sampling, the filter core is sealed and taken back for detection. Thus, that 100 L of water is taken back from each sampling point in traditional detection is avoided; labor intensity is reduced; and detection accuracy is improved. The device is simple in structure and convenient, labor-saving and time-saving to operate, so influence of fatigue of operation personnel on operation during sampling is avoided; the whole process is stable; the device and method overcome the problem of difficulty in accurate controlling of a sampling water volume caused by closing of a switch after artificial observation of scales of a water meter; and result quality is reliable.

Provided herein is a method for reducing down-time in a recreational body of water having a water filtering system and water recirculation system after a potential release of Cryptospridium oocyst and / or Giardia cysts. Also described is a kit for practicing the method for reducing down-time in a recreational body of water that may have a potential release of Cryptospridium oocyst and / or Giardia cysts.

The invention relates to a method and equipment for inactivating Cryptosporidium oocysts and Giardia cysts in water. The inactivation method comprises: the first step of providing water to be treated; the second step of preparing a photocatalyst; and the third step of providing The photocatalyst prepared in the second step is added into the water body obtained in the first step, and the photocatalytic disinfection is carried out on the water body under the condition of light. The equipment used includes: an ultraviolet light source irradiation device, a reactor for containing the photocatalyst and the water solution to be treated, and a stirrer installed in the reactor; the ultraviolet light source irradiation device is arranged on the reactor. The method and equipment have high inactivation efficiency, low cost, simple operation, easy popularization and application, and are non-toxic and by-products, and will not cause secondary pollution.

The invention discloses a method for preparing a filter medium used for removing giardia and cryptosporidium in water. The method comprises the following steps: a) mixing raw materials containing ultra-high molecular weight polyethylene, activated carbon and diatomite in powder, wherein the weight ratio of the ultra-high molecular weight polyethylene, the activated carbon and the diatomite in powder is 100-300:50-100:100-350; and b) pressing, sintering and cooling mixture obtained in the step a) in a mould. Compared with the prior art, the invention has the advantages that the provided technical scheme can remove the giardia and the cryptosporidium in the water, has the removal rate reaching over 99.5 percent so as to achieve the aim of improving the water quality, and has simple method, low cost and quick response.

Oligonucleotide molecules for the detection of Giardia lamblia (G. lamblia which molecules hybridise under medium to high stringency conditions to unique 18S rDNA / rRNA sequences of G. lamblia, and methods for the detection of the presence of viable cells of G. lamblia in samples using the oligonucleotide molecules.

The invention relates to pharmaceutical compositions using a polypeptide comprising at least one CXXC motif, such as Giardia parasite's variable surface proteins (VSP) or a fragment thereof to raise by oral or mucosal vaccination an immune response against a heterologous selected antigen, such as tumor antigen, microbial antigen or other antigen.