30 results about "Giardia species" patented technology

A method for stabilizing and removing Cryptosporidium oocysts or Giardia cysts from water. The method comprises adding chitosan, a salt or solution of chitosan to water containing Cryptosporidium oocysts or Giardia cysts and a halogenating agent. The method may also include adding a secondary polyelectrolyte flocculant to the water. The resulting flocs are filtered to remove the Cryptosporidium oocysts or Giardia cysts.

Raman scattering of radiation applied to a water sample is used to assess occurrence of a pathogen in the sample. The method is useful for detecting pathogens that are difficult to detect using other methods, such as protozoa. Examples of organisms that can be detected in water samples using these methods include protozoa of the genus Cryptosporidium and the genus Giardia. The methods described herein have important applications, such as for detection of Cryptosporidium organisms in municipal water systems.

The invention discloses a sampling filtering bag for cryptospsridium and giardia detection in water and belongs to the field of water quality detection. The structure comprises a shell and filtering paper, wherein the filtering paper is arranged in the shell; a water inlet and a water outlet are formed in the two ends of the shell. The sampling filtering bag is characterized in that the shell is composed of an upper filtering cup and a lower filtering cup; the open end of the upper filtering cup is in butt joint with the open end of the lower filtering cup, and the open end of the upper filtering cup and the open end of the lower filtering cup are sealed by a sealing piece; the water inlet is formed in the top end of the upper filtering cup and the water outlet is formed in the bottom end of the lower filtering cup; the filtering paper is arranged between the open end of the upper filtering cup and the open end of the lower filtering cup. With the adoption of the sampling filtering bag disclosed by the invention, the detection cost can be greatly reduced and the detection recovery rate is improved; the sampling filtering bag has very good popularization and application values.

The invention relates to a filtration and concentration method for improving the detection recovery rate of cryptosporidium and giardia in the technical field of water environmental water quality monitoring. The method comprises the following steps: sampling and filtering; adding elution; ultrasound oscillation and centrifugal treatment; carrying out stained preparation by sequentially adopting animmunomagnetic separation method after completing the centrifugal treatment; and then counting by a fluorescence microscope, and making statistics for the recovery rate. The method improves certain operation steps in the filtration and concentration stages, so as to achieve the purposes of reducing the detection cost of the cryptosporidium and giardia and improving the recovery rate.

The present invention relates to a giardia cyst in water and a cryptosporidium oocyst checking method which belonging to the detection technique field, the giardia and the cryptosporidium in water are detected by the combined-dyeing of haematein and intensified kinyoun after the filtration by microporous membrane and the gradient separation by acetic ether and formalin in a ratio of 10:1. The background complexity of the following microscopic examination is alleviated by membrane filtering the water sample and the giardia and the cryptosporidium are separately dyed by the combined-dyeing of haematein and intensified kinyoun. The invention alleviates the impurity interference in the background in traditional giardia and cryptosporidium detecting method and improves the microscopic examing confirmation accuracy of the morphological and structural features.

A continuous flow centrifuge adapted with an insert of glass beads, sand or the like is used to concentrate cysts of microorganisms such as giardia or cryptosporidium from large volumes of water. The apparatus and methods are useful for the detection of contaminating microoorganisms in groundwater and public drinking water.

The invention relates to a method for estimation of bacterial number in water by particle count in water, and the method belongs to the fields of drinking water processing and sanitation. First, for drinking water with turbidity lower than 1NTU, surface water in rivers and lakes, underground water, and industrial water with turbidity lower than 0.5NTU, a particle calculating instrument is used to determine particle count in certain particle size scopes, and determine bacteria number simultaneously; a certain amount of data is accumulated to satisfy statistical requirement after a plurality of detections, and function relationship between bacterial number and particle count in the certain particle size scope is established; and the function relationship is used to determine bacterial number in water from particle count in the certain particle size scope which is determined by the particle calculating instrument. The estimation method in the invention is simple and fast, can be used for on-line measurement, can determine conventional microorganism indexes such as the total number of bacterium colony, the total number of coliform group bacteria and the like, and can be used for determination of specific microbes such as cryptosporidium, giardia and the like.

The invention relates to the field of public drinking water hygienic security detection and provides a filtering recovery device related to cryptosporidium and giardia for solving the problem that theexisting device for detecting the cryptosporidium and giardia is complicated in detection programs and high in detection cost and a system thereof. The recovery device comprises a left filtering assembly, a right filtering assembly and a filter element, wherein the left filtering assembly comprises a water sample barrel, a water sample peristaltic pump, a left filter cup and a washing liquor collection barrel; a water sample inlet hole and a left filter cup exhaust hole are formed in the top of the left filter cup, and a washing liquor drain hole is formed at the bottom of the left filter cup; the right filtering assembly comprises a washing liquor barrel, a washing liquor peristaltic pump, a right filter cup and a water sample collection barrel; a washing liquor inlet hole and a right filter cup exhaust hole are formed in the top of the right filter cup, and a water sample collection hole is formed in the bottom of the right filter cup; solenoid valves are arranged at the previous holes; the filter element is fixedly arranged between the left filter cup and the right filter cup; the pore size of filter paper at the filter element is less than overall dimensions of the cryptosporidium and giardia.

The invention discloses a method for preparing a filter medium used for removing giardia and cryptosporidium in water. The method comprises the following steps: a) mixing raw materials containing ultra-high molecular weight polyethylene, activated carbon and diatomite in powder, wherein the weight ratio of the ultra-high molecular weight polyethylene, the activated carbon and the diatomite in powder is 100-300:50-100:100-350; and b) pressing, sintering and cooling mixture obtained in the step a) in a mould. Compared with the prior art, the invention has the advantages that the provided technical scheme can remove the giardia and the cryptosporidium in the water, has the removal rate reaching over 99.5 percent so as to achieve the aim of improving the water quality, and has simple method, low cost and quick response.

A transcriptional activator of T. gondii is provided which comprises the tetracycline repressor (TetR) operatively linked to a transacting factor of T. gondii. Strains of T. gondii transformed with a vector containing such a transactivator may be used to prepare vaccine compositions or to identify essential genes in the parasite. The system provided may be useful in other Apicomplexan species such as Plasmodium falciparum, Plasmodium vivax, Plasmodium berghei, Plasmodium yoelii, Plasmodium knowlesi, Trypanosoma brucei, Entamoeba histolytica, and Giardia lambia.

The present invention discloses a combined detection reagent for Cryptosporidium and Giardia, and a preparation method thereof. The agent includes a PVC bottom plate, where a sample pad, a colloidal gold mark pad, a nitrocellulose membrane test pad, and an absorbent pad are disposed on a top of the PVC bottom plate from left to right. In the present invention, two results can be obtained at the same time by detecting two main water-borne parasites (Cryptosporidium and Giardia) that cause human diarrhea and by adding samples for one time, so that not only a detection rate is improved, but alsoa cause of disease is identified. The agent has a fast speed (5-10min), an easy operation, and a stable structure with high sensitivity, strong specificity, good repeatability, and good performance, and is suitable for clinical testing or census, etc., provides rapid screening for patients with low-grade fever or diarrhea after outing or accommodation or eating, to confirm the cause of diarrhea, and can give a diagnosis basis for treatment and medication.

The invention discloses a divalent DNA vaccine for preventing human or animal giardiasis. The preparation method comprises the following steps: respectively inserting two antigen genes alpha1-giardin and CWP-2 of giardia into a eukaryotic vector pVAX1, thereby obtaining recombinant plasmids pVAX1-alpha1-giardin and pVAX1-CWP-2; and respectively transferring the recombinant plasmids pVAX1-alpha1-giardin and pVAX1-CWP-2 into host bacteria, thereby obtaining recombinant bacteria comprising the recombinant plasmids pVAX1-alpha1-giardin and pVAX1-CWP-2 respectively, wherein the recombinant bacteria form the divalent DNA vaccine disclosed by the invention. The anti-giardiasis divalent DNA vaccine in combined application aiming at two stages of trophozoite and cyst respectively is creatively provided, the formation of the trophozoite is inhibited to block the diseases and inhibit formation of the cyst so as to cut off propagation, and the defect that the vaccine is single in effect in the previous vaccine research and development is overcome. According to the divalent DNA vaccine, passage stability and continuous protectiveness are realized. The invention also discloses a quantitative enteric-coated preparation comprising the divalent DNA vaccine and a preparation method of the enteric-coated preparation.