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Method for estimation of bacterial number in water by particle count in water

A particle number and particle counter technology, which is applied in the field of drinking water treatment and sanitation, and can solve the problems that the accuracy of the turbidity meter cannot meet the requirements.

Inactive Publication Date: 2011-06-08
BEIJING UNIV OF TECH
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Problems solved by technology

However, when the turbidity of water is below 1NTU, the accuracy of general turbidimeters often cannot meet the requirements.

Method used

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Examples

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Embodiment Construction

[0013] A micro-polluted water is treated with an ultrafiltration membrane, which is a PVDF hollow fiber membrane with a pore size of 0.01 μm. After ultrafiltration membrane treatment, the water quality is as follows: Turbidity 0.124NTU, Heterotrophic Plate Counting HPC (Heterotrophic Plate Counting) 2.19×10 2 cfu / ml. Total coliforms were 5.3 cfu / ml. The permanganate index is 1.795mg / L. Ammonia nitrogen is 0.391mg / L. The determination of HPC refers to the water and wastewater analysis method jointly compiled by the American Water Supply Association (AWWA), the Water Environment Association (WEF) and the American Public Health Association (APHA). . The turbidity was directly measured with a high-precision turbidimeter. The total coliforms were determined by membrane filtration method. The indicators of total coliform group, permanganate index and ammonia nitrogen all refer to GBT5750.12-2006.

[0014] For the above water samples, an online particle counter was used to det...

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Abstract

The invention relates to a method for estimation of bacterial number in water by particle count in water, and the method belongs to the fields of drinking water processing and sanitation. First, for drinking water with turbidity lower than 1NTU, surface water in rivers and lakes, underground water, and industrial water with turbidity lower than 0.5NTU, a particle calculating instrument is used to determine particle count in certain particle size scopes, and determine bacteria number simultaneously; a certain amount of data is accumulated to satisfy statistical requirement after a plurality of detections, and function relationship between bacterial number and particle count in the certain particle size scope is established; and the function relationship is used to determine bacterial number in water from particle count in the certain particle size scope which is determined by the particle calculating instrument. The estimation method in the invention is simple and fast, can be used for on-line measurement, can determine conventional microorganism indexes such as the total number of bacterium colony, the total number of coliform group bacteria and the like, and can be used for determination of specific microbes such as cryptosporidium, giardia and the like.

Description

technical field [0001] The invention belongs to the field of drinking water treatment and sanitation, and in particular relates to a method for estimating the number of bacteria in water by using the number of particles in water. Background technique [0002] Pathogenic microorganisms exist in water sources such as natural water bodies. If these pathogenic microorganisms in water cannot be effectively controlled, large-scale water-borne infectious diseases may break out. For example, there have been cases in history where residents drank unclean drinking water and caused outbreaks of infectious diseases such as cholera and typhoid fever, resulting in a large number of deaths. Therefore, the removal of pathogenic microorganisms is the focus of drinking water treatment. Since most pathogenic microorganisms are bacteria, the removal and control of bacteria has become an important task in drinking water treatment. my country's drinking water standard (GB-5749-2006) lists micr...

Claims

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Application Information

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IPC IPC(8): C12Q1/06
CPCY02A50/30
Inventor 曹相生孟雪征杨艳玲李星张静相坤
Owner BEIJING UNIV OF TECH
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