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Sampling filtering bag for cryptospsridium and giardia detection in water

A technology of filter capsules and filter cups, which is applied in the field of filter capsules for detection and sampling of two insects in water, can solve the problems that the detection recovery rate is less than 40%, the recovery rate and detection efficiency are not up to the ideal state, and the detection process is cumbersome, etc. The effect of detection recovery, low detection cost, and less eluent volume

Inactive Publication Date: 2014-09-03
SHANDONG GREEN CLEAN ENVIRONMENTAL TESTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, Envirochek method is mostly used to detect "two worms" in the international scope. This method needs to go through five steps: sampling, elutriation, immunomagnetic separation, staining, and microscopic examination. The detection process is cumbersome, and the detection recovery rate is less than 40%.
Although the filter capsule is reusable and has a simpler structure than the Envirochek filter capsule, it still needs to be washed and separated after sampling, and the recovery rate and inspection efficiency are still not ideal

Method used

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  • Sampling filtering bag for cryptospsridium and giardia detection in water
  • Sampling filtering bag for cryptospsridium and giardia detection in water
  • Sampling filtering bag for cryptospsridium and giardia detection in water

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Embodiment

[0021] as attached figure 1 , 2 As shown, the filter capsule for detection and sampling of two insects in water of the present invention is mainly composed of an upper filter cup 1 , a lower filter cup 2 , a sealing rubber ring 3 , a filter paper 4 and a mesh filter bed 5 .

[0022] The open end of the upper filter cup 1 is docked with the open end of the lower filter cup 2, and is sealed and connected by a sealing rubber ring 3.

[0023] The top of the upper filter cup 1 is the water inlet 6, and the bottom of the lower filter cup 2 is the water outlet 7.

[0024] The filter paper 4 and the mesh filter bed 5 are arranged on the open end of the lower filter cup 2 from top to bottom. Between the open end of cup 1 and the open end of lower filter cup 2. The diameter of the filter paper 4 is slightly larger than the diameter of the inner circle of the open end of the upper filter cup 1, and the diameters of the two are 10cm and 9.8cm respectively.

[0025] The filter paper 4 ...

experiment example

[0034] Take 1 part of 100L deionized water and add the standard samples of two worms (Giardia and Cryptosporidium) respectively. There are 100 Giardia and Cryptosporidium in the water sample, for example image 3 As shown, connect the two worm sampling devices, adjust the flow rate to 2000 mL / min, start the peristaltic pump for sampling and filtration, so that the water samples flow out through the filter paper 4, and wait until all the water samples pass through the filter paper 4. After rinsing the upper filter cup with about 1L of pure water, remove the filter paper 4 into a 1000 mL glass stoppered Erlenmeyer flask, add 150 mL of the prepared eluent, cover the sealing plug, and ultrasonically shake for 15 minutes at a temperature of 40 °C . After the ultrasonic vibration stops, open the sealing plug, pour out the eluate into the centrifuge tube, start centrifugal concentration, and finally use the immunomagnetic separation method to stain and prepare slices, count with a fl...

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Abstract

The invention discloses a sampling filtering bag for cryptospsridium and giardia detection in water and belongs to the field of water quality detection. The structure comprises a shell and filtering paper, wherein the filtering paper is arranged in the shell; a water inlet and a water outlet are formed in the two ends of the shell. The sampling filtering bag is characterized in that the shell is composed of an upper filtering cup and a lower filtering cup; the open end of the upper filtering cup is in butt joint with the open end of the lower filtering cup, and the open end of the upper filtering cup and the open end of the lower filtering cup are sealed by a sealing piece; the water inlet is formed in the top end of the upper filtering cup and the water outlet is formed in the bottom end of the lower filtering cup; the filtering paper is arranged between the open end of the upper filtering cup and the open end of the lower filtering cup. With the adoption of the sampling filtering bag disclosed by the invention, the detection cost can be greatly reduced and the detection recovery rate is improved; the sampling filtering bag has very good popularization and application values.

Description

[0001] technical field [0002] The invention relates to the field of water quality detection, in particular to a filter capsule for detection and sampling of two insects in water. Background technique [0003] Giardia parasites in the human small intestine, gallbladder, and duodenum, and can cause symptoms such as abdominal pain, diarrhea, and malabsorption. It is one of the common parasites for human intestinal infection. Cryptosporidium is an important opportunistic pathogenic protozoan that causes diarrhea in humans and animals, and cryptosporidiasis is a zoonotic disease. These two kinds of worms (hereinafter referred to as "two worms") can survive and reproduce in water for a long time, so the "Drinking Water Hygienic Standard" has made strict restrictions on the content of "two worms". [0004] At present, most of the "two worms" are detected by the Envirochek method in the world. This method needs to go through five steps: sampling, washing, immunomagnetic separatio...

Claims

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Application Information

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IPC IPC(8): G01N1/02G01N1/14G01N1/34
Inventor 王虹王冰贺光秀吕亚宁周毅震李剑李丛丛王磊
Owner SHANDONG GREEN CLEAN ENVIRONMENTAL TESTING
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