Filtration and concentration method for improving detection recovery rate of cryptosporidium and giardia

A technology for Giardia and Cryptosporidium, which is applied in the field of filtration and concentration to improve the detection and recovery of Cryptosporidium and Giardia, and can solve the problems of elution of the two worms and the loss of the recovery rate of the two worms

Inactive Publication Date: 2010-03-10
SHANGHAI JIAO TONG UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Further experiments confirmed that it is difficult to completely elute the two worms from the surface of the filter membrane by this mechanical vibration elution, resulting in a large loss of the recovery rate of the two worms

Method used

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  • Filtration and concentration method for improving detection recovery rate of cryptosporidium and giardia
  • Filtration and concentration method for improving detection recovery rate of cryptosporidium and giardia
  • Filtration and concentration method for improving detection recovery rate of cryptosporidium and giardia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Get 1 part of 40L deionized water and add two worms (Cryptosporidium and Giardia) standard sample respectively, in the water sample, each 100 Cryptosporidium and Giardia; figure 1 As shown, put one end of the filter capsule with both ends connected to the rubber tube into the water sample, and connect the other end to the vacuum pump. After ensuring that the water inlet and the water outlet are connected correctly, start the vacuum pump for sampling and filtration, and adjust the flow rate to 2L / min. The water sample flows out through the filter capsule. After all the water samples pass through the filter capsule, remove the rubber tube at the water inlet end of the filter capsule to ensure that the water in the filter capsule does not splash out, and cover the water inlet of the filter capsule with a sealing cover; loosen the filter capsule Let the water sample dry naturally, and cover the water outlet with a sealing cap.

[0023] Remove the sealing cover of the water ...

Embodiment 2

[0027] Get 1 part of 40L deionized water and add two worms (Cryptosporidium and Giardia) standard sample respectively, in the water sample, each 100 Cryptosporidium and Giardia; figure 1 As shown, put one end of the filter capsule with both ends connected to the rubber tube into the water sample, and connect the other end to the vacuum pump. After ensuring that the water inlet and the water outlet are connected correctly, start the vacuum pump for sampling and filtration, and adjust the flow rate to 2L / min. The water sample flows out through the filter capsule. After all the water samples pass through the filter capsule, remove the rubber tube at the water inlet end of the filter capsule to ensure that the water in the filter capsule does not splash out, and cover the water inlet of the filter capsule with a sealing cover; loosen the filter capsule Let the water sample dry naturally, and cover the water outlet with a sealing cap.

[0028] Remove the sealing cover of the water ...

Embodiment 3

[0032] Get 1 part of 40L deionized water and add two worms (Cryptosporidium and Giardia) standard sample respectively, in the water sample, each 100 Cryptosporidium and Giardia; figure 1 As shown, put one end of the filter capsule with both ends connected to the rubber tube into the water sample, and connect the other end to the vacuum pump. After ensuring that the water inlet and the water outlet are connected correctly, start the vacuum pump for sampling and filtration, and adjust the flow rate to 2L / min. The water sample flows out through the filter capsule. After all the water samples pass through the filter capsule, remove the rubber tube at the water inlet end of the filter capsule to ensure that the water in the filter capsule does not splash out, and cover the water inlet of the filter capsule with a sealing cover; loosen the filter capsule Let the water sample dry naturally, and cover the water outlet with a sealing cap.

[0033] Remove the sealing cover of the water ...

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Abstract

The invention relates to a filtration and concentration method for improving the detection recovery rate of cryptosporidium and giardia in the technical field of water environmental water quality monitoring. The method comprises the following steps: sampling and filtering; adding elution; ultrasound oscillation and centrifugal treatment; carrying out stained preparation by sequentially adopting animmunomagnetic separation method after completing the centrifugal treatment; and then counting by a fluorescence microscope, and making statistics for the recovery rate. The method improves certain operation steps in the filtration and concentration stages, so as to achieve the purposes of reducing the detection cost of the cryptosporidium and giardia and improving the recovery rate.

Description

technical field [0001] The invention relates to a method in the technical field of water quality monitoring, in particular to a filtration and concentration method for improving the detection recovery rate of Cryptosporidium and Giardia. Background technique [0002] Cryptosporidium and Giardia are two pathogenic protozoa that seriously endanger water quality. The Hygienic Standard for Drinking Water (GB5749-2006) implemented on July 1, 2007, compared with the previous standard, added the detection of Giardia and Cryptosporidium in the part of microbial indicators, which clearly stipulates that Cryptosporidium , the standard limit of Giardia, that is, <1 / 10L. [0003] After searching the prior art, it is found that the most commonly used standard detection method for Cryptosporidium and Giardia in water in the world is the EPA1623 method. There are three main methods for detecting the two worms (Cryptosporidium and Giardia) with the EPA1623 method. The steps are filtrat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24C12Q1/06C12R1/90
Inventor 何义亮王海亮周云戎颖袁园宋宏臣
Owner SHANGHAI JIAO TONG UNIV
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