Membrane for detecting cattle and sheep Giardia in Haemaphysalis qinghaiensis

Abstract

The invention relates to a membrane for detecting cattle and sheep Giardia in Haemaphysalis qinghaiensis and distinguishing the type. The membrane for detecting Theileria luwenshuni, Theileria uilenbergi and Giardia in cattle and sheep is prepared by fixing specific oligonucleotide of the Theileria luwenshuni and the Theileria uilenbergi or the specific oligonucleotide of Babesia and the universal specific oligonucleotide of Theileria on a Biodyne C membrane. The method for detecting comprises the steps: using DNA extracted from Haemaphysalis to be detected as a template; using a specific primer to perform PCR amplification; hybridizing the product with the oligonucleotide of the Theileria and Babesia for detecting; putting the hybridized membrane in an exposed template holder after enhanced chemiluminescence incubation; putting an X-ray film on the membrane for treatment of exposure, developing and the like; and determining whether the detected animal has the corresponding disease according to an intensity signal produced on the film.

Description

technical field [0001] The invention relates to a detection membrane for detecting whether there are bovine and sheep piriformis in blood ticks in Qinghai, and for distinguishing which kind of piriformis parasitizes sick animals, a preparation method and a use method of the detection membrane. Background technique [0002] Piroplasmosis is a general term for blood protozoa diseases caused by protozoa of Apicomplexa, Piroplasma, Piroplasma Babesco and Tyleridae. Piroplasmosis is also known as "pyroplasmosis" or "blood sporidiosis". It is a blood protozoan disease caused by a variety of piriform worms of the genus Babes in the genus Babesco and the genus Theilaceae transmitted by vector ticks in the red blood cells, and the main clinical features are high fever, anemia, jaundice and hemoglobinuria , can cause death in severe cases. Because there are various ticks distributed all over the world, the distribution of the disease is also very wide. It was first discovered in Egy...

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Problems solved by technology

(2) Apply some serological methods, such as: complement fixation test (CFT) and indirect fluorescent antibody test (IFAT) detection, but these methods are difficult to exclude cross-reactions between different species, and often have false positives and missed detections, See Bruning A. Equine piroplasmosis an update on diagnosis [J]. Br Vet J, 1996, 152: 139-151 and Papadopoulos B, Brossard M, Perie NM (1996) Piroplasms of domestic animals in the Macedonia region of Greece. Piroplasms of small ruminants. VetParasitol 63: 67-74.

However, PCR methods usually cannot detect the occurrence of mixed infections at the same time, see Alhassan A, Pumidonming W, Okamura M, Hirita H, Battsetseg B, Fujisaki F, Yokoyama N, Igarashi I (2005) Development of asingle-round and multiplex PCR method for the simultaneous detection of Babesia cabalian and Babesia equi in horse blood. Vet Parasitol 129:43-49.

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