Combined detection reagent for Cryptosporidium and Giardia and preparation method thereof

Abstract

The present invention discloses a combined detection reagent for Cryptosporidium and Giardia, and a preparation method thereof. The agent includes a PVC bottom plate, where a sample pad, a colloidal gold mark pad, a nitrocellulose membrane test pad, and an absorbent pad are disposed on a top of the PVC bottom plate from left to right. In the present invention, two results can be obtained at the same time by detecting two main water-borne parasites (Cryptosporidium and Giardia) that cause human diarrhea and by adding samples for one time, so that not only a detection rate is improved, but alsoa cause of disease is identified. The agent has a fast speed (5-10min), an easy operation, and a stable structure with high sensitivity, strong specificity, good repeatability, and good performance, and is suitable for clinical testing or census, etc., provides rapid screening for patients with low-grade fever or diarrhea after outing or accommodation or eating, to confirm the cause of diarrhea, and can give a diagnosis basis for treatment and medication.

Description

technical field [0001] The invention relates to the technical field of diarrhea detection, in particular to a cryptosporidium and giardia combined detection reagent and a preparation method thereof. Background technique [0002] Cryptosporidium is a tiny coccidia parasite, which is widely found in a variety of vertebrates. It is mainly Cryptosporidium parvum that parasitizes humans and most mammals. The disease caused by Cryptosporidium parvum is called cryptosporidiosis , is a zoonotic protozoan disease with diarrhea as the main clinical manifestation. The human body is caused by ingestion of drinking water, food and recreational water contaminated by Cryptosporidium oocysts, or close contact with pets, livestock and other animals and wild animals. Infected by contact. [0003] Giardia is referred to as Giardia for short. It parasitizes the human small intestine and gallbladder mainly in the duodenum. It can cause symptoms such as abdominal pain, diarrhea, and malabsorptio...

AI Technical Summary

Problems solved by technology

[0005] At present, there are three types of detection techniques: morphological examination, nucleic acid detection (PCR) and antigen detection. Among them, the advantage of morphological examination is that it is fast, simple and direct, but it is difficult to observe when the number of pathogens is small; the nucleic acid detection procedure is cumbersome, and the sample pretreatment process Many, limited by primer design, nucleic acid purification, reaction conditions, etc., limited to laboratory operations, prone to false positives due to laboratory contamination, sample processing and testing must be performed in an independent space; antigen detection method has strong reaction specificity and is convenient Fast, but its detection efficiency is low. Therefore, we propose a combined detection reagent for Cryptosporidium and Giardia

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