Combined detection reagent for Cryptosporidium and Giardia and preparation method thereof

A technology for Giardia and Cryptosporidium, which is applied in the field of combined detection reagents for Cryptosporidium and Giardia and their preparation, and can solve the problems of multiple sample pretreatment procedures, cumbersome nucleic acid detection procedures, and difficulty in observing pathogens. , to achieve the effect of good performance, stable structure and high sensitivity

Pending Publication Date: 2019-11-15
珠海市医友生物科技有限公司
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Problems solved by technology

[0005] At present, there are three types of detection techniques: morphological examination, nucleic acid detection (PCR) and antigen detection. Among them, the advantage of morphological examination is that it is fast, simple and direct, but it is difficult to observe when the number of pathogens is small; the nucleic acid detection procedure is cumbersome, and the sample pretreatment process Many, limited by primer des

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  • Combined detection reagent for Cryptosporidium and Giardia and preparation method thereof

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Embodiment Construction

[0024] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0025] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "set with", "connected", etc. should be understood in a broad sense, such as "connected", which can be a fixed connection , can also be detachably connected, or integrally connected; can be mechanically connected, can also be electrically connected; can be directly connected, can also be indirectly connected through an i...

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Abstract

The present invention discloses a combined detection reagent for Cryptosporidium and Giardia, and a preparation method thereof. The agent includes a PVC bottom plate, where a sample pad, a colloidal gold mark pad, a nitrocellulose membrane test pad, and an absorbent pad are disposed on a top of the PVC bottom plate from left to right. In the present invention, two results can be obtained at the same time by detecting two main water-borne parasites (Cryptosporidium and Giardia) that cause human diarrhea and by adding samples for one time, so that not only a detection rate is improved, but alsoa cause of disease is identified. The agent has a fast speed (5-10min), an easy operation, and a stable structure with high sensitivity, strong specificity, good repeatability, and good performance, and is suitable for clinical testing or census, etc., provides rapid screening for patients with low-grade fever or diarrhea after outing or accommodation or eating, to confirm the cause of diarrhea, and can give a diagnosis basis for treatment and medication.

Description

technical field [0001] The invention relates to the technical field of diarrhea detection, in particular to a cryptosporidium and giardia combined detection reagent and a preparation method thereof. Background technique [0002] Cryptosporidium is a tiny coccidia parasite, which is widely found in a variety of vertebrates. It is mainly Cryptosporidium parvum that parasitizes humans and most mammals. The disease caused by Cryptosporidium parvum is called cryptosporidiosis , is a zoonotic protozoan disease with diarrhea as the main clinical manifestation. The human body is caused by ingestion of drinking water, food and recreational water contaminated by Cryptosporidium oocysts, or close contact with pets, livestock and other animals and wild animals. Infected by contact. [0003] Giardia is referred to as Giardia for short. It parasitizes the human small intestine and gallbladder mainly in the duodenum. It can cause symptoms such as abdominal pain, diarrhea, and malabsorptio...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531G01N33/543G01N33/569G01N33/577
CPCG01N33/53G01N33/531G01N33/543G01N33/577G01N33/569
Inventor 郭勇华刘现杰
Owner 珠海市医友生物科技有限公司
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