15 results about "Subcellular structure" patented technology

The invention relates to a gastric cancer evaluation system based on a platelet subcellular structure, comprising: a data acquisition module for acquiring a carcino-embryonic antigen level in a blood sample of a detected subject and a platelet proportion in which alpha particles are regularly distributed; the data analysis and judgment module is used for jointly calculating the risk score of the subject suffering from the gastric cancer according to the platelet proportion of'regular distribution 'of alpha particles and the carcino-embryonic antigen level; and the result output module is used for outputting a corresponding gastric cancer assessment result according to the risk score. According to the platelet proportion of'regular distribution 'of alpha particles in a blood sample of a subject and the carcino-embryonic antigen level, the risk score of the subject suffering from the gastric cancer is jointly calculated, the risk of the subject suffering from the gastric cancer is judged, the specificity is 87.5%, and the sensitivity is 87.5%.

The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein and / or nucleic acid expression in a user-defined region of a tissue, user-defined cell, and / or user-defined subcellular structure within a cell.

The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein and / or nucleic acid expression in a user-defined region of a tissue, user-defined cell, and / or user-defined subcellular structure within a cell that are adaptable for use with existing sequencing technologies.

The invention discloses a cationic organic compound as well as a preparation method and application thereof, and belongs to the technical field of organic synthesis. The compound has a novel molecular structure, excellent water solubility, good photophysical properties, high biocompatibility, small molecular weight and outstanding targeting ability. The compound can realize precise dyeing of subcellular structures in cells, especially can specifically locate to mitochondria, does not need a cleaning step in the dyeing process, can obtain a mitochondria image with a high signal-to-noise ratio in a washing-free manner, and provides an efficient and convenient dyeing tool for observation of the subcellular structures.

The invention relates to the technical field of tumor treatment, and discloses a polymeric drug-loaded particle servo type controlled release system and method based on high-frequency oscillation excitation. The system comprises polymeric drug-loaded particles; the Doppler echo repositioning module is used for acquiring imaging information of the tumor tissue in real time and generating a servo-adjusted sound signal according to the imaging information; the array type ultrasonic unit is in communication connection with the Doppler echo re-positioning module and is used for receiving the sound signal subjected to servo adjustment and transmitting high-frequency ultrasound according to the sound signal; wherein when the high-frequency ultrasound acts on the polymeric drug-loaded particles, the polymeric drug-loaded particles are positioned on target tissues under the dual effects of tumor cell surface receptor targeting and subcellular structure targeting, and the sound-sensitive agent generates active oxygen under the excitation of the high-frequency ultrasound, so that the drug-loaded particles can be used for treating tumor cells. And the array type ultrasonic unit can adjust the ultrasonic excitation intensity in real time according to the imaging of the tumor tissue so as to realize the instantaneous active oxygen filling of the target tissue and reconstruct the tumor microenvironment.

The invention relates to the technical field of information, and particularly provides a fish germ cell subcellular structure separation method, which comprises the following steps: obtaining fish gonad tissues in an environment without RNA enzyme pollution, washing with a precooling buffer solution, directly quick-freezing, embedding in an embedding medium, and storing at low temperature; freezing and slicing the quick-frozen and embedded sample to obtain a thin-layer slice, and attaching the thin-layer slice to a membrane subjected to adhesion enhancement treatment and enzyme-free treatment; the attached section is subjected to visual treatment by adopting a specific dyeing combination and then is stored at low temperature; the visualized diaphragm is placed in a humidity-controlled windless enzyme-free environment, and a target membraneless subcellular structure is recognized by combining phase difference or differential interference contrast imaging and three-dimensional stack scanning through a multiple objective lens; the laser microdissection system adopts laser parameters optimized for a small-area target to complete cutting; and adsorbing the cut membraneless subcellular structure into a tube cover of a collecting tube, immediately adding a lysis solution, mixing, and storing at low temperature for nucleic acid extraction.

The invention discloses a TPRN gene therapy system driven by a coProB2 promoter and application of the TPRN gene therapy system, and belongs to the field of biological medicine. Specifically, the invention relates to a nucleic acid, which comprises a coProB2 promoter and mouse TPRN-cDNA, and an expression vector formed by the nucleic acid, and the nucleotide sequence of the coProB2 promoter is SEQ ID NO.1. The invention also relates to a preparation method of the coProB2 promoter. According to the expression vector constructed by the invention, after single administration, the ABR threshold value of a DFNB79 model mouse is recovered to a wild type level (30dB SPL), the curative effect lasts for at least 3 months, the partial rescue effect of the expression vector is obviously superior to that of a broad-spectrum promoter (such as CAG and CMV173), and functional healing is realized; meanwhile, subcellular structure repairing can be achieved, and cross-species safety is achieved.

This invention relates to the field of biomedical image processing technology, specifically a two-dimensional cellular two-photon image analysis system and method, comprising: an image preprocessing subsystem for acquiring and preprocessing calcium imaging video to obtain feature-enhanced images; a subcellular segmentation subsystem for segmenting the image into subcellular structures using a hybrid detection strategy, wherein the hybrid detection strategy consists of manual annotation and adaptive thresholding; and a calcium response feature extraction and analysis subsystem for detecting the fluorescence curve of the calcium response and extracting calcium response event parameter features from the segmented image. This solution can segment astrocytes of different morphologies, distinguish subcellular structures, and employ adaptive thresholding for calcium response detection and analysis, thereby improving segmentation accuracy and analysis efficiency while reducing manual and time costs.

This utility model relates to the field of biological tissue fixation technology, and discloses a device for fixing hollow organs in electron microscopy specimens. The device includes a main body, and further includes a control connection mechanism and a fixation adapter mechanism. The control connection mechanism includes an elastic pick-and-place component located at the upper end of the main body and a connection control component fixedly installed at its lower end. The fixation adapter mechanism includes a conductive support component movably located at the lower end of the connection control component and a fixation adapter component fixedly connected to its lower end. This device for fixing hollow organs in electron microscopy specimens employs a control connection mechanism and a fixation adapter mechanism, which can effectively and stably immerse hollow organ tissues in the fixative, improve the fixation effect, ensure the presentation of subcellular structures, and ensure that the device can be adapted to centrifuge tubes from different manufacturers, effectively meeting the needs of electron microscopy specimen fixation and improving the device's versatility and adaptability.

The present invention relates to, among other things, probes, compositions, methods, and kits for simultaneous, multiplexed detection and quantification of protein expression in a user-defined region of a tissue, user-defined cell, and / or user-defined subcellular structure within a cell.

This invention discloses a system for assessing ovarian cancer recurrence based on super-resolution imaging of platelet subcellular structure. The system includes a data acquisition module, a data analysis module, and an evaluation module. The data acquisition module acquires characteristic values ​​of postoperative ovarian cancer patients and submits them to the data analysis module. These characteristic values ​​include the proportion of platelets with a "regularly distributed" α-granule pattern (X1) and the serum CA125 content (X2). The data analysis module inputs the acquired characteristic values ​​into a multivariate logistic regression model to calculate a joint index (y) and submits it to the evaluation module. The evaluation module assesses the risk of postoperative recurrence based on the principle that a higher value of the joint index (y) indicates a higher risk of recurrence. Compared to the clinically used tumor marker CA125, this evaluation system has higher sensitivity and can detect signs of ovarian cancer recurrence earlier, facilitating early intervention by medical staff.

This invention relates to a method, apparatus, device, and medium for single-cell feature analysis based on hyperspectral coherent Raman scattering imaging, used for single-cell feature analysis. The invention provides an apparatus for performing hyperspectral coherent Raman scattering imaging of samples at different Raman shifts; the method performs hyperspectral coherent Raman imaging on samples enriched with cells using medium A, acquiring images and Raman spectra of different substances at multiple Raman shifts; a multivariate curvature resolution method is used to obtain concentration distribution maps of different substances, and an image is obtained after removing medium A; deep learning technology is used to segment single-cell regions, extracting morphological and metabolic features of cells; simultaneously, a spectral phasor method combined with Lorentz fitting is used to extract morphological and metabolic features of single cells and their subcellular structures. This invention solves the problem of medium structure occlusion, resulting in richer extracted morphological and metabolic features of cells, facilitating more accurate differentiation of cell types.

The invention discloses a single cell, single cell nucleus or single subcellular structure expansion method and application thereof. The expansion method comprises the following steps: dividing raw materials for forming the hydrogel into at least two groups, and respectively introducing the raw materials into a single cell, a single cell nucleus or a single subcellular structure; a step of forming a hydrogel by polymerization; and putting the single cell, single cell nucleus or single subcellular structure with the hydrogel inside into a hypotonic solution to expand. According to the invention, a hydrogel forming region can be controlled in a single cell, single cell nucleus or single subcellular structure, high monodispersity is still maintained after expansion, and a flowable single cell, single cell nucleus or single subcellular structure suspension can be formed; the method can be directly applied to multiple single-cell omics detection platforms such as a microfluidic liquid drop platform, a microwell plate sorting platform and an FACS sorting platform, multiple single-cell omics processes are carried out, the capture efficiency of molecules such as DNA / RNA / protein is improved, and the omics data quality is improved.

The invention discloses a super-resolution imaging method based on chemical reaction luminescence and application, and the method comprises the following steps: marking an imaging target by adopting a specific marking system, and triggering a marking site to generate a chemical reaction through a reaction excitation system in situ to generate a photon signal; the method comprises the following steps: acquiring photon signals generated by chemical reaction by using an imaging system in a continuous multi-frame sampling manner to obtain a data set containing isolated photon spatio-temporal information; image reconstruction is carried out after the data set is preprocessed, information entropy and correlation cumulant are calculated pixel by pixel, and a preliminary super-resolution reconstruction image is obtained based on weighting of the information entropy and the correlation cumulant; and performing post-deconvolution on the preliminary super-resolution reconstructed image to obtain a final super-resolution reconstructed image. According to the invention, the problems of weak reaction luminescence signal and insufficient information utilization are solved, and super-resolution imaging of objects such as protein biomolecules, subcellular structures, bacteria, living organisms, single particles, catalysts and the like is realized.

A method for digitally staining a cell and / or a medical preparation cell comprises: determining three-dimensional information of a cell and / or of a medical preparation using an analyzer for analyzing a medical sample, the analyzer comprising an apparatus for determining the three-dimensional information of the cell and / or of the medical preparation; digitally staining the cell and / or the medical preparation according to a predetermined correlation between the three-dimensional information of the cell and / or of the medical preparation and the staining of a corresponding cell and / or medical preparation and / or cellular and / or sub-cellular structures of the cell and / or of the medical preparation using a staining protocol; representing the digitally stained cell and / or the preparation, the representation involving a predetermined defocus region, and regions of the cell and / or of the preparation being represented by different digital staining in the area of the defocus region as corresponding modulations of color intensities and / or as mixed color.