57 results about "Subcellular structure" patented technology

A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).

Products, compositions, systems, and methods for modifying a target structure which mediates or is associated with a biological activity, including treatment of conditions, disorders, or diseases mediated by or associated with a target structure, such as a virus, cell, subcellular structure or extracellular structure. The methods may be performed in situ in a non-invasive manner by application of an initiation energy to a subject thus producing an effect on or change to the target structure directly or via a modulation agent. The methods may further be performed by application of an initiation energy to a subject in situ to activate a pharmaceutical agent directly or via an energy modulation agent, optionally in the presence of one or more plasmonics active agents, thus producing an effect on or change to the target structure. Kits containing products or compositions formulated or configured and systems for use in practicing these methods.

The present invention discloses an optical clearing agent for bone tissue. The optical clearing agent comprises dimethyl sulfoxide, sodium bicarbonate, and at least two materials selected from polyethylene glycol-400, methyl salicylate, ethylenediaminetetraacetic acid, sodium diatrizoate, glycerol, glucose, sodium dodecylbenzene sulfonate, sorbitol and laurinol, wherein a volume percentage of the dimethyl sulfoxide in the optical clearing agent for bone tissue is 40-80%, a mass percentage of the sodium bicarbonate in the optical clearing agent for bone tissue is 1-2%, the balance is other materials, and the pH value of the optical clearing agent for bone tissue is 6-11. After the optical clearing agent for bone tissue in the present invention acts on bone tissue, a refractive index inside the tissue can be quickly homogenized, and scattering inside the tissue can be reduced, such that the tissue provides improved transparency for light, the imaging depth is increased, and a new method for obtaining cortex neuron subcellular structures and microvascular information is provided. After the optical clearing agent for bone tissue is locally coated on the bone tissues or is adopted to soak the bone tissues, the bone tissue can provide improved transparency for light.

Devices, systems and methods are provided for the targeted treatment of abnormal sensory conditions. In such conditions, physical stimuli is transduced into neuronal impulses that are subsequently transmitted to the central nervous system for processing. Such transduction is achieved by primary sensory neurons in the dorsal root ganglions. Subcellular structures on primary sensory neurons can significantly modulate the function of these neurons, thereby affecting the transduction and reducing the abnormal sensory experiences. Thus, devices, systems and methods are provided for neuromodulating subcellular structures on primary sensory neurons of the dorsal root ganglions.

The present invention relates to methods of measuring electrical properties of a cell using electrode devices comprising tapered nanotips having submicrometer dimensions (“nanoelectrodes”) for insertion into a cell. The devices are used to measure electrical properties of the cell and, optionally, may be used to electroporate, the cell or subcellular structures within the cell. The invention also provides arrays of electrode devices having nanotips for simultaneously or sequentially measuring the electrical properties of cells (e.g., such as surface immobilized cells). The electrodes can be used to measure properties of ion channels and in HTS assays to identify drugs which affect the properties of ion channels. The invention additionally provides microfluidic systems adapted for use with the electrode devices having nanotips. In combination with the electrodes, the microfluidic systems provide cell-based biosensors for monitoring cellular responses to conditions, such as exposure to candidate drugs.

The invention discloses a research method for classifying endosperm tissues in detail by using a morphological method. The research method comprises the following steps: planting corn, sorghum, wheat and rice, experimenting caryopsis developed for different days; examining size, shape and color changes of the caryopsis by using an integrated microscope, and observing position and distribution changes of the endosperm tissues; observing fluorescence characteristics of different tissues of the caryopsis by using a luminescence microscope, and analyzing ingredients of the caryopsis; observing microstructure change of caryopsis and endosperm tissue development by using a light microscope; observing a three-dimensional shape and distribution of cell walls, amyloid, protein body and aleurone cereal by using a scanning electron microscope; and observing characteristics and changes of subcellular structures in cells by using a transmission electron microscope. According to the research method, by using four main cereals as experiment materials, a total development process of the cereal endosperm is analyzed and verified by using various morphological observation technologies, an important role of the endosperm during development of the caryopsis is displayed, and important information is provided for evolution development by comparing structural difference of different grain endosperms.

The present invention provides an analysis method for detecting drug distribution and application thereof, and belongs to the field of drug analysis and detection. The analysis method is as follows: using a fixing treatment method to treat a biological tissue administrated with a to-be-tested drug, and slicing the biological tissue to prepare a sample to be tested; and subsequently analyzing the sample to be tested by using a secondary ion mass spectrometer. Through the analysis method, the distribution of the to-be-tested drug in various tissues, cells and subcellular structures can be known, drug molecules can be detected in cell and subcellular scales, and accumulation of the to-be-tested drug in a target site can be known. The analysis method can be widely used in the study of detection of transport, absorption and metabolism of the drug, or in the study of detection of drug efficacy and drug screening.

The invention discloses a method for measuring cellular oxidative stress damages under ionizing radiation by using fluorescent protein as a fluorescence probe. The content of free radicals generated in ionizing radiation is quantitatively measured by using the fluorescent protein which is selectively positioned and expressed on a subcellular structure sensitive to the ionizing radiation, so that the damages of the ionizing radiation on the specific positions of a cell are speculated by directly observing the fluorescence intensity change of the protein. By the method, the emitted fluorescence is easy to detect, has high sensitivity, stable fluorescence properties, can normally emit light in a wider pH range, has higher resistance to high temperatures, detergents, salt, organic solvents and most ordinary enzymes, is not toxic to the cell, is not interfered by false positive, makes vector construction convenient and is expressed without specificities of species, tissues and positions.

The invention provides a fluorescent probe for detecting superoxide anion free radicals in peroxisome and application of the fluorescent probe, and belongs to the technical field of detection and analysis. The invention designs the fluorescent probe for detecting the superoxide anion free radicals with caffeic acid as a recognition group and a fluorophore of O2<.-> and a small peptide QSKL as a targeted group of the peroxisome, and the structural formula of the fluorescent probe is shown in the description. The fluorescent probe is particularly applicable to detection of the superoxide anion free radicals at a cellular structure level, particularly at a subcellular structure level (such as the peroxisome) and has good actual application value.

The invention belongs to the technical field of growth and reproduction of algae and in particular relates to a micro-fluidic chip for researching the growth and reproduction of the algae. The micro-fluidic chip provided by the invention is composed of a sample channel layer, a valve control layer and a substrate layer, wherein the sample channel layer comprises four parts which are series connected in sequence: a sample inlet, an alga growth observation chamber, a reproduction cell capturing chamber and a sample outlet; alga culture, reproduction cell releasing and automatic separation functions are realized by adopting a continuous pouring technology, and alga cells and sub-cell structures can be observed and recorded for long time in real time; a culture medium of the algae can be rapidly and accurately replaced, and influences, caused by manual intervention, on a growth state of the algae are avoided; raw cells, which are automatically separated, also can be independently cultured.The chip provided by the invention is especially suitable for reproduction and growth researches of various large-size algae. The chip is low in production cost and a method is simple, easy to operate and convenient to popularize.

An alternative illumination source for traditional optical microscopes is provided. This super-resolution illumination source permits to use a traditional optical microscope for the direct observation of objects smaller than 100 nanometers such as subcellular structures in microorganisms, carbon nanotubes and other nanosize objects, and even nanostructures fabricated on top of a silicon wafer. This invention relies on the integration of two functional elements: a microscope, and the super-resolution illumination source. The super-resolution illumination source is formed by an array of light emitting diodes (LEDs) uniformly distributed in a hemisphere. The object under observation is illuminated by the light emitted in all directions by the array of LEDs. Real, wide-field images of the sample with nano-resolution are direct and analogically formed by the microscope's lenses, without the need of sample tagging, intensive computation or scanning. Depending on the wavelength emission of the LEDs, nano-resolution can be obtained with ultraviolet, infrared, and visible illumination.

The present invention belongs to the technical field of biology and discloses an automatic extraction method of cell components. The automatic extraction method comprises the following steps: (1) after mixing a cell sample and a sensitizer, adding the mixture into a sample adding groove for incubation; (2) after incubation for 5-10 min, adding the sample into a filter tube by a pipettor; (3) conducting air injection pressurization and crushing cells under action of shearing force and inertial impact; and (4) if total cellular protein or proteins with a special distribution of a certain subcellular structure need to be collected, pretreating the product obtained in the step (3) and then adding a series of cell component extracts into the filtered product for extraction. The method is different from the traditional method which completely depends on chemical crushing of lysate or grinding and homogenizing method for crushing, so that cell crushing is more uniform, efficiency is high, andprotein damage is small. The method is suitable for processing with small number of samples and batch processing under condition of large total number of the samples, has small requirements on singlesample amount, can effectively save manpower and avoids difference of manual operation.