Fluorescent probe for detecting superoxide anion free radicals in peroxisome and application of fluorescent probe
A peroxisome and superoxide anion technology, applied in the field of detection and analysis, achieves the effects of sensitive detection, simple operation and low cytotoxicity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] A fluorescent probe, the fluorescent probe has the following structure:
[0043]
[0044] Effect verification:
[0045] Optical Properties of Examples of the Invention. Join O 2 ·- After that, the probe (TCP) showed an absorption peak at about 370 nm. ( figure 1 A). Such as figure 1 As shown in B, under single-photon 370nm excitation, the fluorescence intensity of TCP at 495nm is weak, while TCP with 20μMO 2 ·- After incubation together, the fluorescence intensity of the probes at 495nm was significantly enhanced, and the fluorescence intensity was 4 times of the initial fluorescence intensity. Such as figure 1 As shown in C, the fluorescence intensity of TCP at 495 nm is enhanced by more than 8 times under two-photon 800 nm excitation.
[0046] Experiments of the embodiments of the present invention in cells. We first used 0.1 μg / mL 2-Me (to make cells oxidatively stress produce O 2 ·- ) to incubate the PC12 cells for 15 min, then add TCP (20 μM) and in...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com