The invention discloses a footpad skin tissue optical clearing agent, which includes fructose, ethanol, water, and at least three of the following substances: polyethylene glycol-400, sorbitol, glucose, propylene glycol, glycerol, sodium dodecyl benzene sulfonate, dimethyl sulfoxide, butylbenisothiazolene, azone, peppermint oil and petroleum jelly, wherein the mass percentage of fructose in the footpad skin tissue optical clearing agent is 20%-75%, the mass percentage of ethanol in the footpad skin tissue optical clearing agent is 0.8%-1.5%, the mass percentage of water in the footpad skin tissue optical clearing agent is 7%-30%, and the balance of other substances. The present invention acts on the footpad skin tissue, so that the refractive index in the tissue can be quickly uniform, the scattering in the tissue is reduced, the tissue becomes transparent to light, imaging depth is improved, and a new method is provided to obtain information of skin blood vessels and blood flows.