The invention discloses a liquid set for dyeing biological tissue sample slices. The liquid set comprises a slice de-waxing liquid, which comprises D-limonene, methyl cyclohexane, and a surfactant; a hematoxylin dyeing auxiliary liquid, which comprises hematoxylin, anhydrous ethanol, purified water, periodic acid, glycerol, critic acid, and aluminum potassium sulfate; a differentiation liquid, which comprises anhydrous ethanol, purified water, and hydrochloric acid; a bluing liquid, which comprises purified water and a potassium hydroxide water solution; an eosin dyeing liquid, which comprises water soluble eosin, purified water, anhydrous calcium chloride, and a hydrochloric acid water solution; and a slice transparent liquid, which comprises D-limonene, isopropanol, and a surfactant. The provided dyeing liquid set can be used as an environment-friendly pathology/histology reagent for processing tissue samples in pathology department of various hospitals and scientific research institutions, and can be applied to normal pathological diagnosis, immunologic tissue chemical diagnosis technology, molecular biology diagnosis technology, and the effect of tissue processing is better than that of conventional technologies.