Footpad skin tissue optical clearing agent

Abstract

The invention discloses a footpad skin tissue optical clearing agent, which includes fructose, ethanol, water, and at least three of the following substances: polyethylene glycol-400, sorbitol, glucose, propylene glycol, glycerol, sodium dodecyl benzene sulfonate, dimethyl sulfoxide, butylbenisothiazolene, azone, peppermint oil and petroleum jelly, wherein the mass percentage of fructose in the footpad skin tissue optical clearing agent is 20%-75%, the mass percentage of ethanol in the footpad skin tissue optical clearing agent is 0.8%-1.5%, the mass percentage of water in the footpad skin tissue optical clearing agent is 7%-30%, and the balance of other substances. The present invention acts on the footpad skin tissue, so that the refractive index in the tissue can be quickly uniform, the scattering in the tissue is reduced, the tissue becomes transparent to light, imaging depth is improved, and a new method is provided to obtain information of skin blood vessels and blood flows.

Description

technical field

[0001] The invention relates to the technical field of biomedical optical imaging, in particular to a light transparent agent for foot pad skin tissue, which is a mixed solution capable of changing the light transparency of foot pad skin tissue. Background technique

[0002] The footpad is the window of in vivo optical imaging. The hairless and easy-to-obtain characteristics of the footpad skin make it of great significance in the study of tumors and peripheral vascular diseases. The use of optical imaging technology can not only achieve the monitoring and tracking of cells at the single-cell level. However, the opaque nature of the footpad skin limits the depth of light penetration into the tissue, making these techniques only able to image superficial tissues with low image contrast. The recently proposed biological tissue light-transparency technology has brought new ideas to solve this problem. It reduces the scattering of the tissue by introducing a hig...

Examples

specific Embodiment 1

[0021] The isolated footpad skin was taken from a C57BL mouse (8 weeks old), covered it on the 1951USAF resolution plate, and photographed it by CCD. The optical transparent agent is configured according to the ratio of each component of any solution in Example 1 to Example 10 in Table 1, and the mouse foot pad skin tissue is soaked (1.5-2ml / cm 2 ) in the light clearing agent, covering the top of the resolution plate after 10 minutes for shooting. Figure 1 to Figure 3 The resolution plate images taken without skin, with skin and after transparent effect are given respectively. figure 1 Shown is the resolution plate pattern without skin coverage; from figure 2 As can be seen in , the opacity of the footpad skin completely obscures the resolution plate pattern; image 3 It is the case of treating the skin of the foot pad with an optical clear agent for 10 minutes, and it can be seen that the pattern of the resolution plate under the skin coverage can be clearly detected.

specific Embodiment 2

[0022] C57BL mice (8 weeks old) were used for in vivo experiments. The optical transparent agent is configured into a mixed solution according to the ratio of each component of any solution in Example 1 to Example 10 in Table 1, and is directly applied locally on the mouse foot pad skin (0.5-0.8ml / cm 2 ). Figure 4 , Figure 5 The blood flow distribution information of the footpad skin captured by the laser speckle contrast imaging system is given. in Figure 4 The skin blood flow status of the normal C57BL mouse foot pad, due to the turbidity of the skin, the skin blood flow information is difficult to be detected; Figure 5 5 minutes after the skin of the foot pads is partially coated with a light-transparent agent, the skin of the foot pads becomes transparent, and skin blood flow information can be clearly detected.

technical field

[0001] The invention relates to the technical field of biomedical optical imaging, in particular to a light transparent agent for foot pad skin tissue, which is a mixed solution capable of changing the light transparency of foot pad skin tissue. Background technique

[0002] The footpad is the window of in vivo optical imaging. The hairless and easy-to-obtain characteristics of the footpad skin make it of great significance in the study of tumors and peripheral vascular diseases. The use of optical imaging technology can not only achieve the monitoring and tracking of cells at the single-cell level. However, the opaque nature of the footpad skin limits the depth of light penetration into the tissue, making these techniques only able to image superficial tissues with low image contrast. The recently proposed biological tissue light-transparency technology has brought new ideas to solve this problem. It reduces the scattering of the tissue by introducing a hig...

Examples

specific Embodiment 1

[0021] The isolated footpad skin was taken from a C57BL mouse (8 weeks old), covered it on the 1951USAF resolution plate, and photographed it by CCD. The optical transparent agent is configured according to the ratio of each component of any solution in Example 1 to Example 10 in Table 1, and the mouse foot pad skin tissue is soaked (1.5-2ml / cm 2 ) in the light clearing agent, covering the top of the resolution plate after 10 minutes for shooting. Figure 1 to Figure 3 The resolution plate images taken without skin, with skin and after transparent effect are given respectively. figure 1 Shown is the resolution plate pattern without skin coverage; from figure 2 As can be seen in , the opacity of the footpad skin completely obscures the resolution plate pattern; image 3 It is the case of treating the skin of the foot pad with an optical clear agent for 10 minutes, and it can be seen that the pattern of the resolution plate under the skin coverage can be clearly detected.

specific Embodiment 2

[0022] C57BL mice (8 weeks old) were used for in vivo experiments. The optical transparent agent is configured into a mixed solution according to the ratio of each component of any solution in Example 1 to Example 10 in Table 1, and is directly applied locally on the mouse foot pad skin (0.5-0.8ml / cm 2 ). Figure 4 , Figure 5 The blood flow distribution information of the footpad skin captured by the laser speckle contrast imaging system is given. in Figure 4 The skin blood flow status of the normal C57BL mouse foot pad, due to the turbidity of the skin, the skin blood flow information is difficult to be detected; Figure 5 5 minutes after the skin of the foot pads is partially coated with a light-transparent agent, the skin of the foot pads becomes transparent, and skin blood flow information can be clearly detected.

Images