Footpad skin tissue optical clearing agent
A technology of foot pad skin tissue and light transparent agent, which is applied in the field of biomedical optical imaging, can solve the problems that restrict the application of optical imaging technology, achieve the effect of reducing tissue scattering and improving imaging depth
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specific Embodiment 1
[0021] The isolated footpad skin was taken from a C57BL mouse (8 weeks old), covered it on the 1951USAF resolution plate, and photographed it by CCD. The optical transparent agent is configured according to the ratio of each component of any solution in Example 1 to Example 10 in Table 1, and the mouse foot pad skin tissue is soaked (1.5-2ml / cm 2 ) in the light clearing agent, covering the top of the resolution plate after 10 minutes for shooting. Figure 1 to Figure 3 The resolution plate images taken without skin, with skin and after transparent effect are given respectively. figure 1 Shown is the resolution plate pattern without skin coverage; from figure 2 As can be seen in , the opacity of the footpad skin completely obscures the resolution plate pattern; image 3 It is the case of treating the skin of the foot pad with an optical clear agent for 10 minutes, and it can be seen that the pattern of the resolution plate under the skin coverage can be clearly detected.
specific Embodiment 2
[0022] C57BL mice (8 weeks old) were used for in vivo experiments. The optical transparent agent is configured into a mixed solution according to the ratio of each component of any solution in Example 1 to Example 10 in Table 1, and is directly applied locally on the mouse foot pad skin (0.5-0.8ml / cm 2 ). Figure 4 , Figure 5 The blood flow distribution information of the footpad skin captured by the laser speckle contrast imaging system is given. in Figure 4 The skin blood flow status of the normal C57BL mouse foot pad, due to the turbidity of the skin, the skin blood flow information is difficult to be detected; Figure 5 5 minutes after the skin of the foot pads is partially coated with a light-transparent agent, the skin of the foot pads becomes transparent, and skin blood flow information can be clearly detected.
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