Fish tissue pathological slice decalcifying fluid

Abstract

The invention discloses a fish tissue pathological slice decalcifying fluid, which comprises following components: formaldehyde (4-5 v/v%), methanol (50-60 v/v%), formic acid (10-15 v/v%), hydrochloric acid (15-20 v/v%), a tissue clearing reagent (5-6 v/v%), sodium chloride (1-2 v/v%), and the balance being distilled water; wherein the tissue clearing agent comprises following components: 50 to 55vol.% of dimethyl sulfoxide, 15 to 20 mass% of fructose, 1 to 2 mass% of sodium bicarbonate, 10 to 15 mass% of sodium dodecyl sulfonate, and the balance being water. The provided decalcifying fluid can solve the technical problems that in the prior art, the decalcification time is long and the transparency is bad.

Description

technical field [0001] The invention belongs to the technical field of tissue decalcification, and in particular relates to a decalcification liquid for fish histopathological slices. Background technique [0002] The skeleton of a fish is the main structure that supports the body and protects the soft organs. Bone tissue is a hard knotted tissue composed of bone cells and bone matrix. The bone matrix contains a large amount of inorganic salts, mainly hydroxyapatite-calcium phosphate and calcium carbonate. Due to the high hardness of bone tissue, it is easy to detach when slicing. Therefore, in the process of slice making, especially fish brain, gill, and muscle tissue slices, decalcification solution is usually used for decalcification treatment. Its main function is to expand the hoof tissue and soften the bone tissue to facilitate the preparation of stained slices. [0003] At present, the commercially available decalcification solution used to process fish tissue is ex...

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Problems solved by technology

[0005] Aiming at the above-mentioned prior art, the present invention provides a decalcification solution for fish histopathological

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