Tissue specimen fixed decalcifying solution formula

Abstract

The invention relates to a tissue specimen fixed decalcifying solution formula. The decalcifying solution components comprise formaldehyde, formic acid, citric acid, trisodium citrate and water. The decalcifying solution comprises the following components in percentage by weight: 3.8% to 4.2% of formaldehyde, 10% to 30% of formic acid, 5% to 15% of citric acid, 1% to 3% of trisodium citrate and 63% to 68% of water. The decalcification speed is high, the operation is simple, decalcification of tissues at room temperature is completed in 5 to 24 hours, and popularization is easy; weak acid is used for decalcification, the decalcification effect is good, and a tissue fixing function is achieved; and damages to the tissues are little, the influence on the subsequent dyeing and various detection results is small, and professionals can easily accept.

Description

Technical field [0001] The invention relates to the technical field of pathology, in particular to a tissue specimen fixation decalcification solution formula. Background technique [0002] At present, in the field of pathology technology, there are mainly the following four types of decalcification solutions for decalcification of tissue specimens: [0003] 1. Nitric acid decalcification solution: decalcification is fast and has no tissue fixation effect. Nitric acid damages the protein and nucleic acid in the tissue too much, which seriously affects the subsequent tissue staining effect, and causes the subsequent immunohistochemical test and molecular pathological test results to be false negative. Nitric acid is a strong acid with high requirements for transportation and storage. During transportation, storage and use, there are great safety risks to the environment and the safety of operators. [0004] 2. Hydrochloric acid decalcification solution: Decalcification is faster and...

AI Technical Summary

Problems solved by technology

Nitric acid causes excessive damage to proteins and nucleic acids in tissues, seriously affecting subsequent tissue staining results, and causing false negative results in subsequent immunohistochemical and molecular pathological tests

Nitric acid is a strong acid with high requirements for transportation and storage. During transportation, storage and use, there are great safety hazards to the safety of the environment and operators.

[0004] 2. Hydrochloric acid decalcification solution: faster decalcification, no tissue fixation

Hydrochloric acid causes too much damage to proteins and nucleic acids in tissues, which affects the subsequent tissue staining effect and affects the reliability of subsequent immunohistochemical and molecular pathological detection results

Hydrochloric acid is a strong acid with high requirements for transportation and storage. During transportation, storage and use, there are great safety hazards to the safety of the environment and operators.

[0005] 3. Sulfuric acid decalcification solution: fast decalcification, no tissue fixation

However, because EDTA is a chelating agent for decalcification through chelation reaction, the decalcification time takes 3 to 4 weeks, or even longer, so it is difficult to be popularized and used