Method for extracting chitin from American cockroach dregs

A technology of Periplaneta americana and chitin, applied in the field of separation and extraction of natural products, can solve the problems of high requirements for reaction conditions, large environmental pollution, waste, etc., and achieve the effects of cost reduction, good decalcification effect, and simple process

Active Publication Date: 2022-04-29
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is efficient and cheap, and is suitable for almost all chitin sources, but it will produce acid-base waste liquid, which has great environmental pollution; the composition of Periplaneta americana is different from that of shrimp and crab shells, and contains a large amount of fat and protein. Chemical extraction of chitin from shrimp and crab shells in Periplaneta americana, in which the fat, protein and other resources cannot be reused, resulting in waste; there are also studies on using enzymes to replace acid and alkali to extract chitin, but the enzymes are expensive and have little effect on the reaction conditions. The requirements are high, corruption is easy to occur during the reaction process, the obtained product has obvious bitterness and fishy smell, and the production cost is high, so it is not suitable for production and promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting chitin from Periplaneta americana dregs, comprising the following steps:

[0026] S1: Dried Periplaneta americana dregs at 40°C, pulverized, and passed through a 60-mesh sieve to obtain dregs powder;

[0027] S2: Add glucose, yeast extract, and water to the medicinal residue powder and stir evenly to obtain a mixture. The amounts of the medicinal residue powder, glucose, and yeast extract are respectively 4%, 4%, and 0.4% of the volume of the added water. %, after the mixture is sterilized, the medicinal residue culture substrate is obtained;

[0028] S31: Take 2ml of LB broth with a pH of 7.0 and culture it in a 10ml test tube. The composition of the LB broth medium is tryptone 10g / L, yeast extract powder 5g / L, and sodium chloride 10g / L. Sterilize for 15 minutes; get sterilized LB broth medium after cooling;

[0029] S32: Inoculate 2 loops of unrejuvenated Bacillus subtilis on the slant of the test tube containing the sterilized LB broth mediu...

Embodiment 2

[0042] A method for extracting chitin from Periplaneta americana dregs, comprising the following steps:

[0043] S1: Dried Periplaneta americana dregs at 45°C, pulverized, and passed through a 70-mesh sieve to obtain dregs powder;

[0044] S2: Add glucose, yeast extract, and water to the medicinal residue powder and stir evenly to obtain a mixture. The amounts of the medicinal residue powder, glucose, and yeast extract are 5%, 5%, and 0.5% of the volume of the added water, respectively. %, after the mixture is sterilized, the medicinal residue culture substrate is obtained;

[0045]S31: Take 2ml of LB broth with a pH of 7.0 and culture it in a 10ml test tube. The composition of the LB broth medium is tryptone 10g / L, yeast extract powder 5g / L, and sodium chloride 10g / L. Sterilize for 15 minutes; get sterilized LB broth medium after cooling;

[0046] S32: Inoculate 3 loops of unrejuvenated Bacillus subtilis on the slant of the test tube containing the sterilized LB broth mediu...

Embodiment 3

[0059] A method for extracting chitin from Periplaneta americana dregs, comprising the following steps:

[0060] S1: Dried Periplaneta americana dregs at 50°C, pulverized, and passed through an 80-mesh sieve to obtain dregs powder;

[0061] S2: Add glucose, yeast extract, and water to the medicinal residue powder and stir evenly to obtain a mixture. The amounts of the medicinal residue powder, glucose, and yeast extract are respectively 6%, 6%, and 0.6% of the volume of the added water. %, after the mixture is sterilized, the medicinal residue culture substrate is obtained;

[0062] S31: Take 2ml of LB broth with a pH of 7.0 and culture it in a 10ml test tube. The composition of the LB broth medium is tryptone 10g / L, yeast extract powder 5g / L, and sodium chloride 10g / L. Sterilize for 15 minutes; get sterilized LB broth medium after cooling;

[0063] S32: Inoculate 2-3 rings of unrejuvenated Bacillus subtilis on the slant of the sterilized LB broth medium test tube, and cultu...

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PUM

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Abstract

The invention discloses a method for extracting chitin from periplaneta americana dregs. The method comprises the following steps: pretreating the periplaneta americana dregs to obtain dreg powder; adding glucose, yeast extract and water into the medicine residue powder, stirring, and sterilizing to obtain a medicine residue culture medium; inoculating bacillus subtilis into the medicine residue culture medium, and fermenting to obtain bacillus subtilis fermentation liquor; adding glucose into the bacillus subtilis fermentation liquid to obtain a lactic acid fermentation substrate; inoculating lactobacillus into the lactic acid fermentation substrate, and fermenting to obtain lactobacillus fermentation liquor; suspended matters in the lactobacillus fermentation liquor are collected; washing the collected suspended matters to be neutral to obtain neutral suspended matters; adding hydrogen peroxide into the neutral suspended matter for decoloration to obtain a decolorized suspended matter; washing and drying the decolorized suspended matter to obtain chitin; the extraction method disclosed by the invention is simple, environment-friendly and low in cost, and the chitin is high in quality.

Description

technical field [0001] The invention relates to the technical field of separation and extraction of natural products, in particular to a method for extracting chitin from Periplaneta americana dregs. Background technique [0002] The American cockroach (Periplaneta americana) is an insect of the cockroach family. As a traditional Chinese medicinal material, adults are used as medicine. Due to its functions of promoting wound healing and anti-tumor, about 1,000 tons of medicinal materials are extracted and made into preparations for clinical use every year, resulting in a large amount of medicinal residues, which have not been developed and utilized, resulting in a waste of resources. [0003] Chitin, also known as chitin, is the only positively charged animal cellulose found so far in nature. Chitin has a wide range of sources and is distributed in the shells of crustaceans such as shrimps and crabs, insects, and lower plant fungi, as well as in the cell walls of fungi and ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/003C08B37/0003
Inventor 宋芹郭晓强漆文胜李红梅姚倩
Owner CHENGDU UNIV
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