A skull light-transparency reagent and reversible skull tissue light-transparency method

A transparent reagent and skull technology, which can be used in pharmaceutical formulations, preparations for diagnosis using light, and preparations for in vivo tests, etc. function and other issues, to achieve the effect of strong applicability, reducing tissue scattering, and improving imaging depth

Active Publication Date: 2020-05-26
佳维斯(武汉)生物医药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, craniotomy will inevitably bring negative effects, which greatly limits the research on the structure and function of subcortical microvasculature at the body level; the imaging times of thinned cranial windows are limited, and the imaging quality will be affected by the quality of cranial windows. impact; and the surgical operation of thinning and strengthening the cranial window is extremely difficult

Method used

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  • A skull light-transparency reagent and reversible skull tissue light-transparency method
  • A skull light-transparency reagent and reversible skull tissue light-transparency method
  • A skull light-transparency reagent and reversible skull tissue light-transparency method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Step 1. After anesthetizing a two-month-old male Balb / c mouse, shave off the hair on the head of the mouse with a razor blade, cut a two-centimeter-long opening from the eyes to the ears with ophthalmic scissors, and then use a wet cotton ball After gently wiping the mucous membrane on the surface of the skull, dry the surface of the skull with compressed air;

[0057] Step 2: Fix an aluminum fixed piece with holes on the surface of the mouse's unilateral skull with 502 glue, wait for 5-8 minutes for the glue to solidify, and then move the mouse to the mouse positioner and fix its head;

[0058] Step 3, drop the first solution on the surface of the skull, and wipe it off with a cotton ball after reacting for 10 minutes; wherein, the first solution is composed of carboxamide and ethanol with a mass-volume ratio of 30g: 70mL; the concentration of ethanol is 75 %;

[0059] Step 4, drop the second solution on the surface of the skull, and realize the optical transparency o...

Embodiment 2

[0063] Step 1. After anesthetizing eight-month-old male Balb / c mice, shave off the head hair of the mice with a razor blade, cut a two-centimeter-long opening from the eyes to the ears with ophthalmic scissors, and then use a wet cotton ball After gently wiping the mucous membrane on the surface of the skull, dry the surface of the skull with compressed air;

[0064] Step 2: Fix an aluminum fixed piece with holes on the surface of the mouse's unilateral skull with 502 glue, wait for 5-8 minutes for the glue to solidify, and then move the mouse to the mouse positioner and fix its head;

[0065] Step 3, drop the first solution on the surface of the skull, wipe it off with a cotton ball after 15 minutes of reaction, and constantly rub the surface of the skull with an absorbent cotton ball in the process to accelerate the transparent process; wherein, the first solution has a mass-volume ratio of 25g: 75mL of carbonamide mixed with ethanol; the concentration of ethanol is 75%;

...

Embodiment 3

[0070] Step 1. After anesthetizing a two-month-old male Balb / c mouse, shave off the hair on the head of the mouse with a razor blade, cut a two-centimeter-long opening from the eyes to the ears with ophthalmic scissors, and then use a wet cotton ball After gently wiping the mucous membrane on the surface of the skull, dry the surface of the skull with compressed air;

[0071] Step 2: Fix an aluminum fixed piece with holes on the surface of the mouse's unilateral skull with 502 glue, wait for 5-8 minutes for the glue to solidify, and then move the mouse to the mouse positioner and fix its head;

[0072] Step 3, drop the first solution on the surface of the skull, and wipe it off with a cotton ball after reacting for 12 minutes; wherein, the first solution is composed of malonylurea and ethanol with a mass-volume ratio of 28:72; the concentration of ethanol 75%;

[0073] Step 4, drop the second solution on the surface of the skull, and realize the optical transparency of the sk...

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Abstract

The invention discloses a skull optical clearing reagent and a reversible skull tissue optical clearing method, belonging to the technical field of bioimaging. The optical clearing reagent comprises a first solution and a second solution, wherein the first solution is an alcohol solution having the mass concentration being 25-30 % and composed of an amide organic compound and a derivative of the amide organic compound; the second solution comprises a water solution of an anionic surfactant, and a water-soluble penetration enhancer, the mass concentration of the water solution of the anionic surfactant is 15-20 %, and the use amount of the water-soluble penetration enhancer is 5-10 % of the total volume of the second solution. By dropwise adding the first solution and the second solution on the surface of the skull sequentially, the optical transparency of the skull tissue is realized, and the reagent can be used in an optical microscopic imaging system for imaging. With the reagent and the method, the nondestructive and non-invasive transparent skull window is established, the high-resolution imaging of a cortex microvessel system is realized, the operation is simple, the imaging quality is high, and the repeated imaging for a plurality of times can be realized.

Description

technical field [0001] The invention relates to the technical field of biological imaging, in particular to a skull light-transparency reagent and a reversible skull tissue light-transparency method. Background technique [0002] Observation and monitoring of cortical vascular network is an important way to study the structure and function of cerebral cortex microvessels. In recent years, the development of optical imaging technology and the emergence of various labeling technologies have made it possible to observe the changes of cortical vascular morphology and hemodynamic parameters at the living level. Through the real-time and in vivo observation and manipulation of cortical microvasculature, it is expected to reveal the biological laws of cortical microvascular growth, development and microcirculation homeostasis regulation, and to explain the development process and pathogenesis of related brain diseases. and facilitate the development of effective treatments. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61B5/00
CPCA61B5/0059A61B2503/40A61B2503/42A61K49/00
Inventor 张超朱
Owner 佳维斯(武汉)生物医药有限公司
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