A skull light-transparency reagent and reversible skull tissue light-transparency method

Abstract

The invention discloses a skull optical clearing reagent and a reversible skull tissue optical clearing method, belonging to the technical field of bioimaging. The optical clearing reagent comprises a first solution and a second solution, wherein the first solution is an alcohol solution having the mass concentration being 25-30 % and composed of an amide organic compound and a derivative of the amide organic compound; the second solution comprises a water solution of an anionic surfactant, and a water-soluble penetration enhancer, the mass concentration of the water solution of the anionic surfactant is 15-20 %, and the use amount of the water-soluble penetration enhancer is 5-10 % of the total volume of the second solution. By dropwise adding the first solution and the second solution on the surface of the skull sequentially, the optical transparency of the skull tissue is realized, and the reagent can be used in an optical microscopic imaging system for imaging. With the reagent and the method, the nondestructive and non-invasive transparent skull window is established, the high-resolution imaging of a cortex microvessel system is realized, the operation is simple, the imaging quality is high, and the repeated imaging for a plurality of times can be realized.

Description

technical field [0001] The invention relates to the technical field of biological imaging, in particular to a skull light-transparency reagent and a reversible skull tissue light-transparency method. Background technique [0002] Observation and monitoring of cortical vascular network is an important way to study the structure and function of cerebral cortex microvessels. In recent years, the development of optical imaging technology and the emergence of various labeling technologies have made it possible to observe the changes of cortical vascular morphology and hemodynamic parameters at the living level. Through the real-time and in vivo observation and manipulation of cortical microvasculature, it is expected to reveal the biological laws of cortical microvascular growth, development and microcirculation homeostasis regulation, and to explain the development process and pathogenesis of related brain diseases. and facilitate the development of effective treatments. [00...

AI Technical Summary

Problems solved by technology

For example, craniotomy will inevitably bring negative effects, which greatly limits the research on the structure and function of subcortical microvasculature at the body level; the imaging times of thinned cranial windows are limited, and the imaging quality will be affected by the quality of cranial windows. impact; and the surgical operation of thinning and strengthening the cranial window is extremely difficult

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