Transparentizing reagent and application thereof in biological tissue material optical imaging as well as living body skin tissue transparentizing imaging method
A reagent and skin technology, applied in the field of clearing reagents, can solve the problems of low scale processing efficiency, destruction of fine tissue structure, increased damage to cell membranes, etc., and achieve the effect of good transparency rate and transparency effect, reducing tissue scattering, and improving imaging depth.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 29
[0075] Embodiment 29, a kind of living body skin tissue transparent imaging method:
[0076] A. After anesthetizing the mouse, scrape off the ear hair with a razor blade and clean it up.
[0077] B. Fix the mouse with a mouse fixer, stick the mouse ear on the fixer with double-sided tape, and place it at the focal plane of the optical imaging system, fix the dried mouse ear at the imaging position, and image the mouse in two experiments get as Figure 1a , 2a The optical speckle image shown.
[0078] C, drip any clearing agent in embodiment 1-28 to the mouse ear that is fixed on the surface of the fixture, realize the light clearing of skin tissue after 5-30min (especially can use the clearing agent of embodiment 15-28 , to accelerate the transparentization process), it can be used for imaging in an optical microscopic imaging system. In this embodiment, the optically transparentized biological tissue is imaged with a laser speckle imaging system; The internal refractive in...
Embodiment 30
[0083] Embodiment 30. Imaging method for transparent skin tissue in vitro:
[0084] After the mouse was killed under anesthesia, its skin tissue (such as: back skin, mouse ear) was removed with a razor blade, and the hair was shaved off and cleaned up. Soak the isolated mouse ear tissue in the clearing reagent of Example 6 (the main component of which is mannose). After 10-30min, the optical transparency of the isolated tissue of the mouse ear was realized, and the camera was used to take pictures to obtain the following: image 3 As shown in the bright field image, it can be seen from the figure that compared with the clearing effects of PBS, fructose, glycerin, PEG 400, etc. in the prior art, the clearing agent using mannose in the present invention has a good clearing effect, and the 30min After that, the background grid lines behind the mouse ears can be clearly seen, but the complete background grid lines behind the mouse ears cannot be observed by using PBS, fructose, g...
Embodiment 31
[0088] Example 31, application in optical imaging of brain tissue.
[0089]For the fixation and separation of brain tissue, the embodiment adopts mouse brain, and its fixation and separation process is as follows: 20mL needle tubes are filled with paraformaldehyde and PBS (phosphate buffer saline) respectively, and the air bubbles are exhausted. The mice were anesthetized with chloral hydrate at a mass concentration of 10%, and after the mice were completely anesthetized, the mice were fixed with the ventral surface upward. Open the mouse chest cavity to fully expose the heart and liver, and expose the heart as much as possible. Find the apex of the mouse heart, pre-clamp the apex of the heart with hemostatic forceps, hold the needle and insert it from the apex slightly to the right, stop when there is a breakthrough feeling, cut the right atrial appendage, inject PBS, the red blood flows out from the right atrium and rushes out of the whole body, time For about 2-3 minutes, ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com