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Liquid set for dyeing biological tissue sample slices

A technology for section staining and biological tissue, applied in the field of in vitro diagnostic reagents, can solve the problems of poor quality of pathological slides, lack of uniform standards, poor tolerance of reagents, etc., achieve clear tissue cell structure, ensure observation effect, and reduce scattering effects

Inactive Publication Date: 2016-10-26
北京九州柏林生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of toxic chemical reagents such as formaldehyde, xylene, mercuric oxide, and ammonia water are used in the whole process, and pathological technicians are exposed to a toxic environment for a long time; the reagents have poor tolerance, complicated operation, difficult to control, and poor quality of pathological film production, which affects diagnosis; reagent consumption Large, excessive emissions, serious environmental pollution
At the same time, the vast majority of hospitals prepare reagents by themselves, lacking uniform standards

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A biological tissue sample section staining solution, comprising:

[0039] Section dewaxing solution, hematoxylin auxiliary staining solution, hematoxylin staining solution, differentiation solution, bluing solution, eosin staining solution and section clearing solution;

[0040] The slice dewaxing solution includes: D-limonene 60g, methylcyclohexane 40g and surfactant 0.01g;

[0041] The hematoxylin auxiliary dye solution includes: 0.01 g of surfactant and 0.01 g of saturated citric acid solution, and the pH value of the hematoxylin auxiliary dye solution is 5.5;

[0042] The hematoxylin staining solution includes: 7g hematoxylin, 100g absolute ethanol, 1000g purified water, 0.25g periodic acid, 85g glycerol, 5g citric acid and 50g aluminum potassium sulfate;

[0043] Differentiation solution includes: absolute ethanol 55g, purified water 45g and hydrochloric acid 0.3g;

[0044]The bluing solution includes: 10000g of purified water and 500g of aqueous potassium hydro...

Embodiment 2

[0048] A biological tissue sample section staining solution, comprising:

[0049] Section dewaxing solution, hematoxylin auxiliary staining solution, hematoxylin staining solution, differentiation solution, bluing solution, eosin staining solution and section clearing solution;

[0050] The slice dewaxing solution includes: D-limonene 60g, methylcyclohexane 40g and surfactant 0.03g;

[0051] The hematoxylin auxiliary dye solution includes: 0.03g of surfactant and 0.01g of saturated citric acid solution, wherein the pH value of the hematoxylin auxiliary dye solution is 5.5;

[0052] The hematoxylin staining solution includes: 7g of hematoxylin, 100g of absolute ethanol, 1000g of purified water, 0.3g of periodic acid, 85g of glycerol, 5g of citric acid and 50g of potassium aluminum sulfate;

[0053] Differentiation solution includes: absolute ethanol 55g, purified water 45g and hydrochloric acid 0.5g;

[0054] The bluing solution includes: 10000g of purified water and 500g of ...

Embodiment 3

[0058] Preparation of hematoxylin staining solution and eosin staining solution in Example 1 and Example 2

[0059] Preparation of hematoxylin staining solution: adding hematoxylin to absolute ethanol, stirring evenly, to obtain the first premix; adding aluminum potassium sulfate to purified water, stirring evenly, to obtain the second premix; heating the second premix to boiling, and Add the first premix to the second premix, stir evenly, boil for 10-15 minutes, add periodic acid and glycerol, stir evenly, cool to 20-30°C, add citric acid to obtain a hematoxylin staining solution.

[0060] Preparation of eosin staining solution: add anhydrous calcium chloride to purified water, stir evenly, then add water-soluble eosin and 0.1% hydrochloric acid aqueous solution by volume, stir evenly, and let stand for 24 hours to obtain eosin staining solution .

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Abstract

The invention discloses a liquid set for dyeing biological tissue sample slices. The liquid set comprises a slice de-waxing liquid, which comprises D-limonene, methyl cyclohexane, and a surfactant; a hematoxylin dyeing auxiliary liquid, which comprises hematoxylin, anhydrous ethanol, purified water, periodic acid, glycerol, critic acid, and aluminum potassium sulfate; a differentiation liquid, which comprises anhydrous ethanol, purified water, and hydrochloric acid; a bluing liquid, which comprises purified water and a potassium hydroxide water solution; an eosin dyeing liquid, which comprises water soluble eosin, purified water, anhydrous calcium chloride, and a hydrochloric acid water solution; and a slice transparent liquid, which comprises D-limonene, isopropanol, and a surfactant. The provided dyeing liquid set can be used as an environment-friendly pathology / histology reagent for processing tissue samples in pathology department of various hospitals and scientific research institutions, and can be applied to normal pathological diagnosis, immunologic tissue chemical diagnosis technology, molecular biology diagnosis technology, and the effect of tissue processing is better than that of conventional technologies.

Description

technical field [0001] The invention relates to in vitro diagnostic reagents, in particular to a dyeing solution for biological tissue sample sections. Background technique [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, as long as there is an operation, the pathology department of the hospital will make a histopathological diagnosis on the surgical tissue samples. Conventional paraffin-embedded HE staining is still the most basic technique currently used to process tissue samples. It is the basis of pathological diagnostic methodology and the reference standard for evaluating the pros and cons of many new methods and technologies. At present, the traditional method is still used in the whole country and even the whole world, that is, the tissue samples are placed in the tank of the processing liquid, poured or transferred into different processing liquids in sequence, and the samples are soaked in each reagent for different time ...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 李明王德田
Owner 北京九州柏林生物科技有限公司
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