Liquid set for dyeing biological tissue sample slices

A technology for section staining and biological tissue, applied in the field of in vitro diagnostic reagents, can solve the problems of poor quality of pathological slides, lack of uniform standards, poor tolerance of reagents, etc., achieve clear tissue cell structure, ensure observation effect, and reduce scattering effects

Inactive Publication Date: 2016-10-26
北京九州柏林生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large amount of toxic chemical reagents such as formaldehyde, xylene, mercuric oxide, and ammonia water are used in the whole process, and pathological technicians are exposed to a toxic environment for a long time; the reagents have poor tolerance, complicated operation, diff

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0037] Example 1,

[0038] A dye kit for biological tissue sample slices, including:

[0039] Section dewaxing solution, hematoxylin auxiliary staining solution, hematoxylin staining solution, differentiation solution, blueing solution, eosin staining solution and section clear solution;

[0040] The slice dewaxing solution includes: D-limonene 60g, methylcyclohexane 40g and surfactant 0.01g;

[0041] Hematoxylin auxiliary dye solution includes 0.01g surfactant and 0.01g saturated citric acid solution, and the pH value of hematoxylin auxiliary dye solution is 5.5;

[0042] Hematoxylin staining solution includes: hematoxylin 7g, absolute ethanol 100g, purified water 1000g, periodic acid 0.25g, glycerol 85g, citric acid 5g and aluminum potassium sulfate 50g;

[0043] The differentiation solution includes: 55 g of absolute ethanol, 45 g of purified water and 0.3 g of hydrochloric acid;

[0044] The blue liquid includes: 10000g purified water and 500g potassium hydroxide aqueous solution with...

Example Embodiment

[0047] Example 2,

[0048] A dye kit for biological tissue sample slices, including:

[0049] Section dewaxing solution, hematoxylin auxiliary staining solution, hematoxylin staining solution, differentiation solution, blueing solution, eosin staining solution and section clear solution;

[0050] The slice dewaxing solution includes: 60 g of D-limonene, 40 g of methyl cyclohexane and 0.03 g of surfactant;

[0051] Hematoxylin dye-assisted solution includes 0.03g surfactant and 0.01g saturated citric acid solution, wherein the pH of hematoxylin dye-assisted solution is 5.5;

[0052] Hematoxylin staining solution includes: hematoxylin 7g, absolute ethanol 100g, purified water 1000g, periodic acid 0.3g, glycerol 85g, citric acid 5g and aluminum potassium sulfate 50g;

[0053] The differentiation solution includes: 55 g of absolute ethanol, 45 g of purified water and 0.5 g of hydrochloric acid;

[0054] The blue liquid includes: 10000g purified water and 500g potassium hydroxide aqueous solut...

Example Embodiment

[0057] Example 3

[0058] Preparation Example 1 and Example 2 Hematoxylin staining solution and eosin staining solution

[0059] Prepare hematoxylin dyeing solution: add hematoxylin to absolute ethanol and stir evenly to obtain the first premixed solution; add potassium aluminum sulfate to purified water and stir evenly to obtain the second premixed solution; heat the second premixed solution to boiling, Add the first premix to the second premix, stir evenly, boil for 10-15 minutes, add periodic acid and glycerol, stir evenly, cool to 20-30°C, add citric acid to obtain hematoxylin dyeing solution.

[0060] Prepare eosin dyeing solution: add anhydrous calcium chloride to purified water, stir well, then add water-soluble eosin and 0.1% hydrochloric acid aqueous solution with volume percentage, stir evenly, and let stand for 24h to obtain eosin dyeing solution .

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Abstract

The invention discloses a liquid set for dyeing biological tissue sample slices. The liquid set comprises a slice de-waxing liquid, which comprises D-limonene, methyl cyclohexane, and a surfactant; a hematoxylin dyeing auxiliary liquid, which comprises hematoxylin, anhydrous ethanol, purified water, periodic acid, glycerol, critic acid, and aluminum potassium sulfate; a differentiation liquid, which comprises anhydrous ethanol, purified water, and hydrochloric acid; a bluing liquid, which comprises purified water and a potassium hydroxide water solution; an eosin dyeing liquid, which comprises water soluble eosin, purified water, anhydrous calcium chloride, and a hydrochloric acid water solution; and a slice transparent liquid, which comprises D-limonene, isopropanol, and a surfactant. The provided dyeing liquid set can be used as an environment-friendly pathology/histology reagent for processing tissue samples in pathology department of various hospitals and scientific research institutions, and can be applied to normal pathological diagnosis, immunologic tissue chemical diagnosis technology, molecular biology diagnosis technology, and the effect of tissue processing is better than that of conventional technologies.

Description

technical field [0001] The invention relates to in vitro diagnostic reagents, in particular to a dyeing solution for biological tissue sample sections. Background technique [0002] The birth of histopathology has a history of more than 150 years. In all hospitals, as long as there is an operation, the pathology department of the hospital will make a histopathological diagnosis on the surgical tissue samples. Conventional paraffin-embedded HE staining is still the most basic technique currently used to process tissue samples. It is the basis of pathological diagnostic methodology and the reference standard for evaluating the pros and cons of many new methods and technologies. At present, the traditional method is still used in the whole country and even the whole world, that is, the tissue samples are placed in the tank of the processing liquid, poured or transferred into different processing liquids in sequence, and the samples are soaked in each reagent for different time ...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 李明王德田
Owner 北京九州柏林生物科技有限公司
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