Research method for classifying endosperm tissues in detail by using morphological method

A morphological and endosperm technology, applied in material analysis by optical means, material analysis by measuring secondary emissions, scientific instruments, etc., can solve the problem of insufficient development of endosperm, and achieve the effect of enriching evidence and data

Inactive Publication Date: 2015-03-04
YANGZHOU UNIV
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Problems solved by technology

However, the development of endosperm, especially the diff

Method used

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  • Research method for classifying endosperm tissues in detail by using morphological method
  • Research method for classifying endosperm tissues in detail by using morphological method
  • Research method for classifying endosperm tissues in detail by using morphological method

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Experimental program
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Embodiment

[0028] Experimental materials and methods

[0029] Plant corn, sorghum, wheat and rice, and take caryopsis with different days of development for experiments:

[0030] (1) Stereo microscope observation of samples

[0031] Cut the caryopsis longitudinally or transversely, take the longitudinal section directly or through TTC (2,3,5-triphenyltetrazolium chloride) and I 2 -KI (iodine-potassium iodide) staining and observation with a stereomicroscope (Leica MZ6).

[0032] (2) Fluorescence microscope observation of samples

[0033] The caryopsis was cut longitudinally by hand or with a frozen microtome (Leica CM1100), and the fluorescence characteristics of the caryopsis tissue were directly observed under a fluorescence microscope (Leica DMLB) using the principle of autofluorescence of cellulose.

[0034] (3) Light microscope and transmission electron microscope observation of the sample

[0035] Cut the caryopsis longitudinally, take the part to be observed, first fix it with...

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Abstract

The invention discloses a research method for classifying endosperm tissues in detail by using a morphological method. The research method comprises the following steps: planting corn, sorghum, wheat and rice, experimenting caryopsis developed for different days; examining size, shape and color changes of the caryopsis by using an integrated microscope, and observing position and distribution changes of the endosperm tissues; observing fluorescence characteristics of different tissues of the caryopsis by using a luminescence microscope, and analyzing ingredients of the caryopsis; observing microstructure change of caryopsis and endosperm tissue development by using a light microscope; observing a three-dimensional shape and distribution of cell walls, amyloid, protein body and aleurone cereal by using a scanning electron microscope; and observing characteristics and changes of subcellular structures in cells by using a transmission electron microscope. According to the research method, by using four main cereals as experiment materials, a total development process of the cereal endosperm is analyzed and verified by using various morphological observation technologies, an important role of the endosperm during development of the caryopsis is displayed, and important information is provided for evolution development by comparing structural difference of different grain endosperms.

Description

technical field [0001] The invention belongs to the field of biological science and agricultural science theory and technology, and is a set of feasible plant morphology research scheme. Background technique [0002] Morphological observation technology is a very important research method in the fields of botany, zoology and medicine. The more common methods in China include stereoscopic microscope observation, fluorescence microscope observation, light microscope observation, scanning electron microscope observation and transmission electron microscope observation. However, it is almost rare in China that can make comprehensive use of these observation techniques to conduct in-depth exploration of special plant tissues with clear layers and strict logic. [0003] Maize, sorghum, wheat, and rice are four major cereals whose caryopsis are widely used as human food, livestock feed, and industrial raw materials. Endosperm is an important nutrient transport and storage tissue i...

Claims

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Application Information

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IPC IPC(8): G01N21/84G01N21/64G01N23/22G01Q30/02
Inventor 郑彦坤王忠
Owner YANGZHOU UNIV
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