Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Research method for classifying endosperm tissues in detail by using morphological method

A morphological and endosperm technology, applied in material analysis by optical means, material analysis by measuring secondary emissions, scientific instruments, etc., can solve the problem of insufficient development of endosperm, and achieve the effect of enriching evidence and data

Inactive Publication Date: 2015-03-04
YANGZHOU UNIV
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the development of endosperm, especially the differentiation process of endosperm, is not well studied today

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Research method for classifying endosperm tissues in detail by using morphological method
  • Research method for classifying endosperm tissues in detail by using morphological method
  • Research method for classifying endosperm tissues in detail by using morphological method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] Experimental materials and methods

[0029] Plant corn, sorghum, wheat and rice, and take caryopsis with different days of development for experiments:

[0030] (1) Stereo microscope observation of samples

[0031] Cut the caryopsis longitudinally or transversely, take the longitudinal section directly or through TTC (2,3,5-triphenyltetrazolium chloride) and I 2 -KI (iodine-potassium iodide) staining and observation with a stereomicroscope (Leica MZ6).

[0032] (2) Fluorescence microscope observation of samples

[0033] The caryopsis was cut longitudinally by hand or with a frozen microtome (Leica CM1100), and the fluorescence characteristics of the caryopsis tissue were directly observed under a fluorescence microscope (Leica DMLB) using the principle of autofluorescence of cellulose.

[0034] (3) Light microscope and transmission electron microscope observation of the sample

[0035] Cut the caryopsis longitudinally, take the part to be observed, first fix it with...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a research method for classifying endosperm tissues in detail by using a morphological method. The research method comprises the following steps: planting corn, sorghum, wheat and rice, experimenting caryopsis developed for different days; examining size, shape and color changes of the caryopsis by using an integrated microscope, and observing position and distribution changes of the endosperm tissues; observing fluorescence characteristics of different tissues of the caryopsis by using a luminescence microscope, and analyzing ingredients of the caryopsis; observing microstructure change of caryopsis and endosperm tissue development by using a light microscope; observing a three-dimensional shape and distribution of cell walls, amyloid, protein body and aleurone cereal by using a scanning electron microscope; and observing characteristics and changes of subcellular structures in cells by using a transmission electron microscope. According to the research method, by using four main cereals as experiment materials, a total development process of the cereal endosperm is analyzed and verified by using various morphological observation technologies, an important role of the endosperm during development of the caryopsis is displayed, and important information is provided for evolution development by comparing structural difference of different grain endosperms.

Description

technical field [0001] The invention belongs to the field of biological science and agricultural science theory and technology, and is a set of feasible plant morphology research scheme. Background technique [0002] Morphological observation technology is a very important research method in the fields of botany, zoology and medicine. The more common methods in China include stereoscopic microscope observation, fluorescence microscope observation, light microscope observation, scanning electron microscope observation and transmission electron microscope observation. However, it is almost rare in China that can make comprehensive use of these observation techniques to conduct in-depth exploration of special plant tissues with clear layers and strict logic. [0003] Maize, sorghum, wheat, and rice are four major cereals whose caryopsis are widely used as human food, livestock feed, and industrial raw materials. Endosperm is an important nutrient transport and storage tissue i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/84G01N21/64G01N23/22G01Q30/02
Inventor 郑彦坤王忠
Owner YANGZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com