87 results about "Antibiotics therapy" patented technology

The invention discloses a nano-antibacterial agent for treating biofilm infection through Prussian blue and a preparation method and application thereof.According to the nano-antibacterial agent, hollow Prussian blue (HPB) serves as a carrier to load a substance with anti-inflammatory and anti-oxidation effects, a metal polyphenol network structure is formed through chelating of tannic acid (TA) modified by hyaluronic acid (HA) and high-valence metal ions, and the nano-antibacterial agent is used for treating biofilm infection through Prussian blue. And the MPNs are packaged to obtain the product. All the components of the nano antibacterial agent have a synergistic effect, MPNs formed by chelating TA modified by hyaluronic acid and high-valence metal ions in the antibacterial agent have good photo-thermal performance, and light energy can be converted into heat energy for photo-thermal therapy. According to the invention, the nanoparticles for photo-thermal antisepsis and anti-inflammation are constructed, so that comprehensive treatment of anti-biofilm and anti-inflammatory effect is realized, and healing of infected parts is promoted. And the antibacterial agent has good biocompatibility and high biological safety, and is very suitable for replacing antibiotics to treat biological membrane infection.

The invention discloses production and application of an antibacterial protein targeting enterococcus pluvialis. Specifically, the antibacterial protein comprises an amino acid sequence as shown in any one of SEQ ID NO.15-28 or an active fragment and an analogue of the amino acid sequence. According to the present invention, the potential antibacterial protein is found from the genome of the bacteria or the bacteriophage, and the antibacterial protein can inhibit the enterococcus pluvialis in the targeting manner; the antibacterial protein with biological activity can be obtained in a heterologous expression mode and can remove the enterococcus pluvialis in a targeted mode, and then related diseases caused by the enterococcus pluvialis are relieved and treated. The antibacterial protein provided by the invention can perform targeted sterilization without damaging normal human symbiotic flora, and can avoid side effects caused by traditional antibiotic treatment.

The invention belongs to the technical field of microbial antibacterial preparations, and particularly discloses a novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6 and application of the novel strong vibrio parahaemolyticus bacteriophage isolated strain vBVpSCC6. The bacteriophage vBVpSCC6 is preserved in the Guangdong Microbial Culture Collection Center on September 14, 2020, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 61189-B1. The bacteriophage has good tolerance to environments such as temperature, pH, ultraviolet rays and the like, and has a good prevention and control effect on aquatic product pollution caused by vibrio parahaemolyticus. The bacteriophage has a lysis effect on multi-drug-resistant vibrio parahaemolyticus, can replace antibiotics to play a bacteriostatic role, effectively solves the problem of drug resistance faced by current antibiotic treatment, and has relatively good utilization value and application prospect. The bacteriophage can also be prepared into a composition with other bacteriophages to play a synergistic effect, and the bacteriophage can be applied to wider-spectrum antibacterial or bacteriostatic application.

The invention provides a test strip for identifying gram-negative bacteria in a peritoneal dialysis solution and a preparation method, and relates to the technical field of test strips. The test strip comprises a bottom plate as well as a sample pad, a combination pad, a coating pad and a water absorption pad which are sequentially arranged on the bottom plate, wherein a polydopamine coated colloidal gold coupled antibiotic probe is sprayed on the combination pad; a detection line is arranged on the coating pad, and the detection line is formed by hydrogel coated with antibiotics. According to the non-antibody-dependent immunochromatography test paper for detecting the gram-negative bacteria in the peritoneal dialysis solution, provided by the invention, a marker is simple and convenient to prepare, the use of paired antibodies is avoided, and the cost of the test paper is further reduced; moreover, time is gained for clinical diagnosis of microbial pathogens in dialysate, and correspondingly, antibiotic treatment is carried out on a patient in time. Each detection process can be completed within 10 min, the detection result is accurate, and the gram-negative bacteria in a peritoneal dialysis solution sample can be rapidly detected.

The invention provides an antibacterial material based on a nano-enzyme synergistic sonodynamic curative effect and a preparation method and application. The antibacterial material comprises BiPt nano-enzyme, the outer side of the BiPt nano-enzyme is coated with BSA, PpIX is connected to the BSA, BPBP nano-particles are formed, the outer side of the BPBP nano-particles is coated with AP hydrogel, and a BPBP (at) AP antibacterial material is obtained. The BPBP (at) AP nano antibacterial material disclosed by the invention is combined with ultrasound, the bacteriostasis rate on helicobacter pylori exceeds 99%, a biological membrane is removed, and the risk caused by antibiotic treatment is avoided.

The present disclosure relates generally to methods of treating infections in overweight or obese patients using antibiotics.

Compounds and methods for combinatorial drug discovery in nanoliter droplets are described herein. More particularly, novel synergistic agents that increase efficacy of antibiotic agents to treat bacterial infections are described.

The invention belongs to the field of food nutrition and pharmacy, and particularly relates to folium mori crude polysaccharide and application thereof. An extract rich in mulberry leaf polysaccharide is extracted from mulberry leaves through a specific extraction process, and it is found through an in-vitro antibacterial experiment that the crude mulberry leaf polysaccharide has the direct inhibiting and killing effect on helicobacter pylori (Helicobacter pylori). In-vitro intestinal flora fermentation research shows that the folium mori crude polysaccharide can effectively correct intestinal flora disorder caused by triple antibiotics for treating helicobacter pylori, meanwhile, the short-chain fatty acid level is remarkably improved, and the helicobacter pylori abundance in intestinal flora is reduced. The application field of mulberry leaf polysaccharide is widened, and important reference is provided for developing products for adjuvant therapy of H.pylori infection and intestinal microecology regulation based on natural polysaccharide.

The invention relates to the technical field of traditional Chinese medicine composition preparation and application, and discloses a traditional Chinese medicine composition for improving urinary tract infection and a preparation method and application thereof.The traditional Chinese medicine composition is prepared from radix bupleuri, radix scutellariae, raw radix astragali, radix salviae miltiorrhizae, the root of Chinese pulsatilla, herba patriniae, polygonum aviculare, chingma abutilon seeds, the root of three-nerved spicebush, fennel, cinnamon and juncus roemerianus. The traditional Chinese medicine composition comprises the following components in parts by mass: 14-16g of radix bupleuri; 9 to 11 g of radix scutellariae; 19 to 21 g of raw radix astragali; 29 to 31 g of red sage root; 13-17g of Chinese pulsatilla root; 28-32 g of herba patriniae; 14-16 g of polygonum aviculare; 13 to 16 g of chingma abutilon seeds; 7-9g of combined spicebush root; 7-9g of fennel; 5-7g of cinnamon; according to the traditional Chinese medicine composition for strengthening the body resistance, clearing heat and promoting diuresis, the clearing curative effect on RUTI drug-resistant bacteria is better than that of antibiotics, the symptoms of a patient are obviously improved, the recurrence rate is lower than that of an antibiotic group, and the treatment principle and purpose of traditional Chinese medicine are completely different from those of antibiotic treatment; the traditional Chinese medicine composition disclosed by the invention considers recovery and improvement of the overall functional state of a patient instead of purely removing pathogenic bacteria.

This invention provides the application of gonococcal ferritin in the preparation of anti-gonococcal products, relating to the field of antibody technology. The application of gonococcal ferritin subunits in the preparation of antibodies against gonococcal ferritin inhibits gonococcal infection by intervening in the iron metabolism pathway of gonococci, without inducing classic drug resistance mutations. This overcomes the limitations of existing technologies that rely on antibiotics, achieving broad-spectrum inhibition of drug resistance lineages. It solves the technical problem in existing technologies where multidrug / pan-drug resistance of gonococci leads to complete failure of antibiotic treatment, and the lack of therapeutic targets targeting its core survival mechanisms.

The invention discloses a culture method based on posthypoxia gastric intima separation treatment of freshwater white pomfret, and relates to the technical field of aquaculture and disease prevention and control. The method comprises the following steps: step 1, identifying hypoxia symptoms; 2, medicine compatibility feeding is carried out; 3, regulating and controlling the water body environment; 4, breeding environment management; and step 5, continuous monitoring. According to the invention, the food maternal, the endothelium corneum gigeriae galli and the pipemidic acid are combined for the separation treatment of the endogastric membranes of the aquatic animals for the first time, the thinking limitation of traditional disease prevention and treatment is broken through, the traditional Chinese and western medicine theories are organically combined, and a new disease prevention and treatment thought is provided for the aquaculture industry; compared with a traditional pure antibiotic treatment or water quality improvement method, the scheme is high in pertinence and directly aims at a core pathological link of gastric intima separation after hypoxia; the comprehensive treatment effect is good, and meanwhile, anti-infection, mucous membrane repair and function regulation are considered; and the used medicines are all common medicines, have small side effects and low residues, and meet the safety requirements of aquatic products.

The invention relates to an in vitro method for determining whether a newborn patient has late-onset neonatal sepsis, which comprises determining the PTX-3 protein concentration in a biological sample previously obtained from said patient.

Disclosed herein are compounds, compositions, and methods for treating infections by bacteria and for killing bacteria. In particular, the disclosed compounds, compositions, and methods are useful for treating infections by bacteria and for killing persister bacteria that survive antibiotic treatment and then reconstitute infections.

Blood and lung infection is a worldwide health problem, and about five millions of cases of death each year. Early treatments can help rescue life but require better tests to identify these infections. The invention recognizes pathogens, in particular those having antibiotic resistance, more quickly. The invention allows appropriate antibiotic treatment to be performed at an earlier point in time. Data from deep RNA sequencing of human blood is used to create a faster diagnostic test against infection and related antimicrobial resistance.

The application describes treatment of hepatic encephalopathy using gastrointestinal specific antibiotics. One example of a gastrointestinal specific antibiotic is rifaximin. The instant application also provides methods for determining if a subject has a neurological condition or hepatic encephalopathy by determining the critical flicker frequency and / or the venous ammonia level of the subject at two or more time points. The invention further provides methods for treating these subjects.

The present invention relates to compositions and methods for preventing and / or treating bacterial disease (e.g., disease caused by Neisseria spp. such as Neisseria gonorrhoeae or Neisseria meningitidis). In particular, the present invention provides compositions comprising an effective amount of a nucleic acid, wherein such compositions are capable of killing or inhibiting the growth of a Neisseria spp and sensitizing Neisseria spp to antibiotic treatment.

The invention discloses a gelled cell as well as a preparation method and application thereof. Relates to the field of synthetic biology and biomedicine. The gelated cell is a cell structure without cell viability, and comprises: a carrier cell framework comprising a cell membrane which retains the membrane protein content, the type and the lipid order of an original carrier cell; the intracellular hydrogel is formed in situ in the carrier cell framework through supramolecular host-guest interaction or cross-linking reaction, and is fused with the inner surface of a cell membrane; the antibacterial substance is loaded in the intracellular hydrogel; wherein pores are distributed on the cell membrane, the intracellular hydrogel is communicated with the external environment through the pores, and the loaded antibacterial substances are exposed. The gelated cell provided by the invention can simultaneously realize targeted delivery, contact sterilization and endotoxin adsorption, and avoids the problem of secondary inflammation caused by release of endotoxin in traditional antibiotic therapy and the problem of membrane protein loss or disordered arrangement in a cell membrane coating technology.

The invention belongs to the field of foods or medicines, and particularly relates to a probiotic and prebiotic composition, a preparation process and application thereof. The composition comprises probiotics, resistant dextrin, xylooligosaccharide, fructo-oligosaccharide, stachyose, erythritol and cranberry powder, and is prepared by a fluidized bed spray granulation process. As an auxiliary preparation of antibiotics, animal and trial administration tests prove that the composition can synergistically treat upper / lower respiratory tract infection and urinary tract infection, shorten the symptom duration, accelerate the recovery of original diseases, maintain the diversity of intestinal flora, improve the abundance of beneficial bacteria such as bifidobacteria and reduce the incidence rate of adverse reactions, and can be used for preparing the medicine for treating the diseases. The antibiotic-related diarrhea is relieved. The invention provides a micro-ecological restoration scheme for antibiotic treatment, and has clear clinical value and process feasibility.

The invention discloses a feed additive for replacing antibiotics to treat chicken necrotic enteritis and application of the feed additive, and belongs to the technical field of feed. The effective component of the feed additive is the tannin extract from chestnut trees, and animal experiments prove that the tannin extract from chestnut trees can improve the production performance of broiler chickens with necrotic enteritis to a certain extent, improve the intestinal morphology and immune function of the broiler chickens with necrotic enteritis and improve the immunity of the broiler chickens with necrotic enteritis. Therefore, the chestnut tree tannin extract can replace antibiotics and has a good treatment effect on broiler intestinal inflammation caused by clostridium perfringens and coccidiosis.

The invention discloses a bacteriostasis-related protein as well as a biological material and application thereof. The technical problem to be solved by the invention is how to inhibit bacteria. The invention specifically discloses application of the G enzyme. The application is any one of the following items: A1) application in preparation of a product for promoting wound healing; a2) in preparation of a product for promoting healing of infectious wounds; a3) in preparation of a product for promoting and / or assisting in promoting wound healing; a4) in preparation of a product for enhancing the antibacterial and / or bactericidal ability of antibiotics; a5) application in preparation of a product for enhancing the ability of antibiotics to treat or / and prevent eye infection; a6) in improving the cell activity and / or preparing a product for improving the cell activity. The G enzyme is any one of the following items: B1) the amino acid sequence is a protein of a sequence 1 or 31-442 sites of the sequence 1; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same activity with the protein B1); b3), a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The G enzyme disclosed by the invention has an obvious antibacterial effect when being used for gram-negative bacteria, and can be used for industrial production.

The invention relates to the technical field of biological medicines, in particular to mRNA (messenger Ribonucleic Acid) for coding a single-chain antibody resisting staphylococcus aureus and application of the mRNA. On the basis of a specific scFv with high affinity to staphylococcus aureus lysate, cell wall protein and toxin, the mRNA is optimized by a gene sequence and is matched with a functional element suitable for in-vivo expression of the mRNA, so that stable and efficient expression of the mRNA in a target tissue is ensured. The mRNA is further prepared into an mRNA-LNP preparation, and the preparation is beneficial to delivery of the mRNA to local tissues of the mammary gland of the dairy cow, expresses scFv protein with a specific antibacterial function in epithelial cells of the mammary gland, can be used for replacing traditional antibiotics to treat the mastitis of the dairy cow caused by staphylococcus aureus, and has an outstanding treatment effect.

Compositions of hyaluronic acid (HA) conjugated with antibiotics are disclosed for a rapid release antibiotic delivery system into a closed space to achieve rapid therapeutic range within 24 hours and effective elimination of the antibiotic within 80-120 hours. In particular, this invention is directed to compositions, method of preparation, and method of treatment to prevent invention using porous and non-porous embodiments of hyaluronic acid polymer conjugated with antibiotics or other therapeutic agents for rapid therapeutic delivery and controlled reduction of therapeutic dosages of antibiotics to prevent invention and reduce antibiotic resistance. Low concentration of crosslinkers conjugated with HA as well as using porogens and functional group modifiers allow other therapeutic to be conjugated to HA such as sugars and steroids. Suspension of HA conjugates in chitosan enable additional treatment means. Anti-cancer therapeutic agents may also be conjugated for controlled release using this system.

The invention discloses a process for treating burns and scalds without using antibiotics. The process comprises the following steps: S1, setting a treatment environment: carrying out the whole burn and scald treatment process in a sterile room; s2, heat-dissipating and pain-relieving operation: spraying an antibacterial, pain-relieving and heat-dissipating spray on the wound surfaces of the burns and scalds every 5-10 minutes; s3, wound surface treatment measures: preparing special medicine patch gauze for wound surface treatment; 30% of alcohol lithospermum liquid, 15% of pseudo-ginseng liquid and a proper amount of alcohol are blended into a 75-degree alcoholic solution, gauze is soaked to prepare the medicine patch, the medicine patch is directly applied to burn and scald wounds, the wounds are kept in an unbound state, it is ensured that the wounds make full contact with air, heat dissipation and drying are facilitated, and the bacterium breeding environment is reduced. The problem of bacterial drug resistance and adverse drug reaction caused by use of antibiotics are effectively avoided, the body burden of patients is relieved, and the traditional Chinese medicine composition is beneficial to long-term health of the patients.

Methods for identifying and providing information about inhibiting growth of polymicrobial infections, including but not limited to providing statistics or information about the likelihood of success in inhibiting growth of a polymicrobial infection with particular compositions or therapeutic solutions. The methods herein feature detection and identification of organisms of the polymicrobial sample (e.g., polymicrobial infection), phenotypic pooled sensitivity tests for determining the susceptibility or resistance of the polymicrobial sample (e.g., polymicrobial infection) in the sample to an antibiotic or other therapeutic agent, and identification of resistance genes, e.g., genetic markers that may indicate resistance to a particular treatment. Together, the data can be applied against databases of antibiotic / therapeutic susceptibility or resistance for particular known polymicrobial samples (e.g.. polymicrobial infections) in order to provide information related to the likelihood of success of one or more therapeutic solutions for the polymicrobial sample (e.g.. polymicrobial infection).

According to the screening method of the natural antibiotic-free medicine for preventing and treating methicillin-resistant staphylococcus aureus, the provided screening method can screen out the optimal medicine composition and proportion, and the screened medicine serves as an alternative scheme of antibiotic treatment; and a medicine screening and research and development foundation is laid for treating infection of methicillin-resistant staphylococcus aureus. The method for rapidly screening the natural drug monomer composition aiming at the methicillin-resistant staphylococcus aureus is provided by comprehensively applying a minimum inhibitory concentration experiment and an orthogonal method, and the method is low in cost, less in time consumption and simple to operate. The method provided by the invention verifies that the three natural drug monomers of baicalin, emodin and caffeic acid have a synergistic effect, and meanwhile, when the three drug monomers of baicalin, emodin and caffeic acid are screened according to the method provided by the invention and are combined according to the mass ratio of 2: 1: 4, the antibacterial effect on methicillin-resistant staphylococcus aureus is the best.

Provided herein are rapid and effective local infection therapy methods, systems, and devices that significantly reduce the mortality, morbidity, and the cost of care in rare musculoskeletal infections. Continuous delivery of antibiotic therapy locally, at the infection site, reduces edema and provides antibiotic irrigation, significantly improving outcomes while reducing the need for systemic antibiotics.

The present invention provides a method of identifying an animal for which antibiotic treatment, anti-infective treatment, probiotic treatment, immunostimulant treatment, additional monitoring and / or a separate or alternative management strategy is appropriate, comprising: a) collecting a sample from said animal; b) performing a leukocyte differential cell count on said sample; c) comparing said leukocyte differential cell count of (b) with an index of infection; and d) initiating antibiotic treatment, anti-infective treatment, probiotic treatment, immunostimulant treatment, additional monitoring and / or a separate or alternative management strategy of said animal on the basis of said comparing step.

The present application belongs to the technical field of microbial prevention and treatment, and particularly relates to a mycobacterium phage and application thereof. Specifically, the present application successfully screens a mycobacterium phage MP1 capable of precisely targeting and killing mycobacterium marinum, which can precisely target and kill specific mycobacterium marinum without causing adverse effects on normal flora. This feature enables the phage therapy to avoid the adverse reactions and flora imbalance problems caused by antibiotic treatment when treating drug-resistant mycobacterium marinum. Meanwhile, the above-mentioned mycobacterium phage MP1 is obtained from nature and is easy to be industrialized, and a drug or environmental disinfectant prepared from the above-mentioned phage not only can reduce the cost, but also has the advantages of green environmental protection and the like, and therefore has good practical application value.

The invention discloses a preparation method of a nano chitosan antibacterial synergist, which comprises the following steps: dissolving chitosan in acetic acid, and standing overnight after dissolving until no obvious particles exist to obtain a chitosan solution; adding an antibacterial drug into the chitosan solution, performing magnetic stirring, and then adding a cross-linking agent to obtain the nano chitosan antibacterial synergist. The nano chitosan conjugated penicillin is used for the first time, the effect of the nano chitosan conjugated penicillin on clinical drug-resistant escherichia coli is verified, application possibility is provided for reducing use and drug residues of clinical drugs, and compared with a current common antibiotic treatment method, the nano chitosan conjugated penicillin has the advantages that the application range is wide; the preparation method has the advantages that the antibacterial effect is improved, the drug use amount is reduced, drug residues are reduced, the drug resistance problem is solved, and the preparation method is simple and convenient. According to the research, on the basis of drug-resistant escherichia coli, nano chitosan antibacterial synergists with different concentrations are added, and the antibacterial effect of the nano chitosan antibacterial synergists on the drug-resistant escherichia coli is evaluated. And a certain theoretical and experimental basis is provided for clinical medication.

Provided herein are postbiotic compositions prepared using successive fermentation methods of specific bacteria and plant fiber material. Also provided herein are methods, including for the treatment or prevention of the disruption of gut microbiota, or dysbiosis, associated with an antibiotic treatment, chemotherapy treatment, or administration of a dysbiosis-causing medications or medical treatments, using said postbiotic compositions, or for improving responses and / or reducing complications to treatments such as antibiotics or chemotherapy. Such postbiotic compositions can also be used for the treatment or prevention of the disruption of the gut-brain axis, including to treat or prevent neurological diseases or disorders, such as synucleinopathies, including but not limited to Parkinson's disease.