Immunofluorescent labeling method for protein in living cell

Abstract

The invention relates to the technical field of biology, in particular to an immunofluorescent labeling method for protein in a living cell. The method comprises the following steps of coupling an antibody fragment of protein to be labeled and a fluorescent dye to obtain a fluorescently-labeled antibody fragment; binding liposome and the fluorescently-labeled antibody fragment to obtain a transportable label; culturing a cell to be labeled and the transportable label in a culture medium, transporting the transportable label into the cell to be labeled by utilizing the characteristic that the liposome can be fused with a cell membrane, namely the membrane permeability of the liposome in a culturing process, and performing specific immune binding on the transportable label and the protein to be labeled in the cell to be labeled through an antigen binding fragment on the protein to be labeled, thereby connecting the fluorescent dye to the protein to be labeled to finally fulfill the aim of immunofluorescent labeling of the protein in the living cell.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for immunofluorescence labeling of proteins in living cells. Background technique [0002] Immunofluorescence labeling is an effective method for specifically labeling intracellular proteins. It is a technique for labeling antibodies with fluorescent dyes for antigen localization. This method has high specificity for labeling proteins, and fluorescent dyes are often chemically synthesized dyes with high quantum efficiency. [0003] However, the immunofluorescence labeling method in the related technology can only label fixed cells (first damage the living cells and then label), and cannot label the proteins in living cells, so this method cannot be used to observe the dynamic changes of living cells. For further biological research. Summary of the invention [0004] The purpose of the present invention is to provide a method for immunofluorescence labeling of proteins in l...

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Problems solved by technology

[0003] However, the immunofluorescence labeling method in the related art can only label fixed cells (the living cells are damaged first and then labeled)

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