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Preparation carried with particles of antitumor drug and preparation method thereof

A technology of anti-tumor drugs and preparations, which is applied in the field of preparations loaded with anti-tumor drug particles, which can solve the problems of necrosis of mesenchymal stem cells, achieve the effects of improving curative effect, broad application prospects, and reducing toxic and side effects

Inactive Publication Date: 2011-10-26
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is still no effective technology to solve the problem of mesenchymal stem cell necrosis caused by the accumulation of anti-tumor drugs and the transport of anti-tumor drug particles to malignant tumors and local sustained release of drugs

Method used

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  • Preparation carried with particles of antitumor drug and preparation method thereof
  • Preparation carried with particles of antitumor drug and preparation method thereof
  • Preparation carried with particles of antitumor drug and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Adriamycin Hydrochloride Microparticles

[0033] a. Preparation of anti-tumor drug microparticles: prepared by emulsification-solvent evaporation method. The preparation process is as follows: doxorubicin hydrochloride and PLA are dissolved in DMSO to form an organic phase. The above organic phase was emulsified by ultrasound in pH 7.4 phosphate buffer solution containing PVA205, and the organic solvent was evaporated quickly by drying under reduced pressure to prepare doxorubicin hydrochloride PLA with an average particle size of 120nm and a drug content of 2.5%. particle.

[0034] b. Preparation of multidrug-resistant mesenchymal stem cells expressing P-glycoprotein: using retroviruses as vectors, transfecting multidrug-resistant genes into mesenchymal stem cells to obtain multidrug-resistant mesenchymal stem cells expressing P-glycoprotein Stem cells, spare.

[0035] The preparation process is as follows: use liposome transfection method t...

Embodiment 2

[0037] Example 2 Preparation of paclitaxel-loaded microparticles

[0038] a. Preparation of anti-tumor drug microparticles: prepared by emulsification-solvent evaporation method. The preparation process is as follows: paclitaxel and PLA are dissolved in dichloromethane to form an organic phase. The above organic phase was emulsified by ultrasound in a pH 7.4 phosphate buffer containing Tween 80, and the organic solvent was quickly evaporated by drying under reduced pressure to prepare paclitaxel PLA particles with an average particle size of 95nm and a drug content of 4.1%.

[0039] b. Preparation of multidrug-resistant mesenchymal stem cells expressing P-glycoprotein: using lentivirus as a carrier, transfecting multidrug-resistant genes into mesenchymal stem cells to obtain multidrug-resistant mesenchymal stem cells expressing P-glycoprotein Stem cells, spare; the preparation process is as follows: import pMX-mdr1-GFP and outer membrane protein particles PCI VSV and PCIPGB i...

Embodiment 3

[0041] Example 3 Formulations Carrying Docetaxel Microparticles

[0042] a. Preparation of anti-tumor drug microparticles: prepared by emulsification-solvent evaporation method. The preparation process is as follows: docetaxel and PLGA are dissolved in acetone to form an organic phase. The above organic phase was emulsified by ultrasound in pH 7.4 phosphate buffer solution containing poloxamer 188, and the organic solvent was quickly evaporated by drying under reduced pressure to prepare polysaccharides with an average particle size of 80 nm and a drug content of 0.01%. Paclitaxel PLGA microparticles, wherein the molar ratio of propiolactone to betaine in PLGA is 1 / 99;

[0043] b. Preparation of multidrug-resistant mesenchymal stem cells expressing P-glycoprotein: using retroviruses as vectors, transfecting multidrug-resistant genes into mesenchymal stem cells to obtain multidrug-resistant mesenchymal stem cells expressing P-glycoprotein Mesenchymal stem cells, spare;

[00...

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Abstract

The invention provides a preparation carried with particles of antitumor drug and a preparation method thereof, belonging to the technical field of medicine. The preparation uses multidrug resistant mesenchymal stem cells as carrier of particles of antitumor drug, the multidrug resistant mesenchymal stem cells are multidrug resistant mesenchymal stem cells of a P-glycoprotein-expressing. According to the invention, the problem of necrocytosis of mesenchymal stem cells caused by antineoplastic drug accumulation is solved well, the particles of antitumor drug can be transported to a malignant tumor and released slowly in local, so that the targeted therapy of tumor is realized, curative effect is improved, toxic and side effect is reduced, and the invention has a wide application prospect.

Description

technical field [0001] The invention relates to a pharmaceutical preparation, in particular to a preparation loaded with antineoplastic drug particles, which belongs to the technical field of medicine. Background technique [0002] Targeted therapy for malignant tumors has always been a hot spot of common concern for medical workers at home and abroad. Mesenchymal stem cells (MSCs) are non-hematopoietic stem cells with multi-lineage differentiation potential. In addition to the characteristics of hematopoietic support, immune regulation, and multi-lineage differentiation, they also have the ability to specifically migrate to injury sites and tumor tissues. characteristic. Recent studies have shown that mesenchymal stem cells can be transfected with adenovirus, retrovirus and lentivirus without changing the biological characteristics of mesenchymal stem cells. Therefore, tumor-targeted gene therapy using mesenchymal stem cells as cell carriers can specifically migrate to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K47/46C12N5/10A61P35/00
Inventor 高子彬胡洁张丽男孙勇军申宁张莉李硕谢英花吴韶梅刘磊姚军杨恩芸
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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