Nanometer therapeutic agent with effects of molecule targeting and controllable photodynamic therapy and preparation method and application thereof

A photodynamic therapy and molecular targeting technology, applied in the field of biomedicine, can solve the problems of poor curative effect and increased toxicity

Inactive Publication Date: 2018-07-24
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the premature release of nanomedicine before reaching the tumor site cannot be avoided, resulting in poor efficacy and increased toxicity.

Method used

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  • Nanometer therapeutic agent with effects of molecule targeting and controllable photodynamic therapy and preparation method and application thereof
  • Nanometer therapeutic agent with effects of molecule targeting and controllable photodynamic therapy and preparation method and application thereof
  • Nanometer therapeutic agent with effects of molecule targeting and controllable photodynamic therapy and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Measure 20 mL of ultrapure water into a 50 mL round-bottomed flask, add 2.0 g of cetyltrimethylammonium chloride (CTAC), stir at room temperature until completely dissolved, add 0.2 mg of triethanolamine (TEA), and dissolve at 95 Heat and condense in an oil bath at constant temperature and stir for 1 h. Then, 1.5 mL of tetraethylsilane (TEOS) was added dropwise, and then heated, condensed and stirred at a constant temperature in an oil bath at 95 °C for 1 h. The sample was cooled to room temperature, and washed 3-4 times by high-speed centrifugation with absolute ethanol to remove residual reaction reagents. Then collect the samples in 20 mL of washing solvent (0.2 g sodium chloride dissolved in 20 mL of methanol), stir at room temperature for 3 h, wash with high-speed methanol centrifugation for 3-4 times to remove the template agent CTAC, and freeze-dry to obtain white MSN solid powder.

[0041] Take 100 mg of MSN and dissolve them together with 2.5 mL of 3-aminopro...

Embodiment 2

[0043] Take by weighing 600 mg embodiment 1 gained MSN-NH 2 The solid powder was dissolved in 50 mL of methanol, then 5 mg of ICG was added, stirred at room temperature in the dark for 8 h, centrifuged at 10,000 rpm for 10 min, the obtained precipitate was washed three times with ethanol and secondary water, and then the obtained product was freeze-dried, namely Get MI.

Embodiment 3

[0045] Weigh 2.2 g of zinc acetate and 220 mg of magnesium acetate (Zn:Mg=10:1), dissolve in 150 mL of absolute ethanol, heat and stir at 50 °C until they dissolve. Weigh 500 mg of NaOH, dissolve it in 30 mL of absolute ethanol, heat and stir at 70 °C, and cool it in an ice bath after it is completely dissolved, then quickly add it to a flask containing zinc acetate and magnesium acetate, and keep it in an ice bath Under the reaction 8h. After the reaction was completed, n-hexane was added to generate a precipitate. Centrifuge at 8000 rpm for 5 min. Then the solid was dissolved in absolute ethanol again, n-hexane was added, centrifuged again, and the above steps were repeated 3 times, and the obtained product was dried in a fume hood to obtain ZnO QDs.

[0046] Weigh 500 mg ZnO QDs, dissolve them in 75 mL DMF, dissolve them by ultrasonication, then add 250 µL APTES, react at 120 °C for 15 min, centrifuge at 8000 rpm for 5 min, and wash the product 3 times with DMF. The prod...

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Abstract

The invention discloses a novel erlotinib-chitosan-mesoporous silica nanoparticle-indocyanine green (ECMI) nanometer therapeutic agent and a preparation method and application thereof. The nanometer therapeutic agent uses mesoporous silica nanoparticles loaded with indocyanine green as carriers in the interior, utilizes ZnO QDs to block the pores on the surfaces of the mesoporous silica nanoparticles, and coats the surfaces of the mesoporous silica nanoparticle with an erlotinib-modified chitosan disulfide crosslinked polymer. The nanometer therapeutic agent can identify lung cancer cells belonging to different molecular classes and is used for controllable near-infrared fluorescence imaging. Through molecular targeting to drugs and photodynamic therapy, the therapeutic effect on lung cancer is improved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to an erlotinib-chitosan-mesoporous silica-indocyanine green novel nano-diagnostic agent (ECMI) and its preparation method and application. Background technique [0002] Lung cancer is a cancer with high morbidity and mortality in the world, and its 5-year survival rate is only 15%. Lung cancer is divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). NSCLC is the most common type, accounting for 85% of all lung cancers. Epidermal growth factor receptor (EGFR) is a transmembrane protein that belongs to the epidermal growth factor receptor family. Studies have found that in NSCLC patients, EGFR binding to ligands can lead to phosphorylation of tyrosine kinases, induce the secretion of tumor growth factors, and promote tumor cell proliferation and angiogenesis. The human EGFR gene is located in the p13-q22 region of chromosome 7, with a to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K49/00A61K31/517A61K47/61A61P35/00A61P11/00
CPCA61K31/517A61K41/0057A61K49/0034A61K49/005A61K49/0054A61K49/0063
Inventor 高瑜张英英柯玲洁林晓文张露李冬梅方胤瑾雷桂财陈海军
Owner FUZHOU UNIV
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