The invention discloses a therapeutic recombinant antibody, and an encoding gene and application thereof. In an excellent embodiment, the therapeutic recombinant antibody is mainly formed by fusion of CBD collagen specific-binding polypeptide and an Fab segment of cetuximab, and contains a light-chain variable region formed by a genetic code with a sequence shown by SEQ ID NO:1 and a heavy-chain variable region which is formed by a genetic code with a sequence shown by SEQ ID NO:2. The therapeutic recombinant antibody disclosed by the invention not only has the specific-binding capacity of collagen but also reserves the EGFR binding capacity of the cetuximab, and besides, the functions of killing tumor cells and suppressing tumor growth of the cetuximab are also maintained; and in addition, the recombinant antibody has high targeting ability and good tumor tissue infiltration capacity in bodies and can realize slow release in tumor tissues, the anti-tumor effects of the therapeutic recombinant antibody are far better than those of a total-length antibody namely the cetuximab, and the therapeutic recombinant antibody has wide application prospects in tumor detection preparations, antitumor medicines and the like.