Construction method for ion chromatography fingerprint spectrums of ganoderma lucidum spore powder polysaccharide

A technology of Ganoderma lucidum spore powder and ion chromatography is applied in the field of fingerprints of traditional Chinese medicines and their products, functional food raw materials and their products, and can solve the problems of single hydrolysis selectivity, insufficient information on oligosaccharide components, and detection sensitivity of oligosaccharide components. It is easy to master, the method is stable, and the cost is high.

Active Publication Date: 2012-08-22
中食都庆(山东)生物技术有限公司
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Problems solved by technology

However, the patented method of pre-column PMP derivation-reversed-phase HPLC fingerprints only uses acid hydrolysis, the hydrolysis selectivity is single, and the detection sensitivity of oligosaccharide components in the PMP derivation-reversed-phase HPLC analysis is not high, so the method fingerprints Insufficient information on oligosaccharide components reflecting structural properties

Method used

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  • Construction method for ion chromatography fingerprint spectrums of ganoderma lucidum spore powder polysaccharide
  • Construction method for ion chromatography fingerprint spectrums of ganoderma lucidum spore powder polysaccharide
  • Construction method for ion chromatography fingerprint spectrums of ganoderma lucidum spore powder polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, the establishment method of polysaccharide ion chromatography standard fingerprint of ganoderma lucidum spore powder

[0055] 1. Instruments, reagents and samples

[0056] 1.1 Instrument

[0057] ICS5000 multifunctional ion chromatography (Dionex, USA), pulsed amperometric electrochemical detector.

[0058] 1.2 Reagents and samples

[0059] Monosaccharide standard products: glucose (Glc, 99%), mannose (Man, 99%), galactose (Gal, ≥99%) were purchased from Shanghai Chemical Reagent Company; rhamnose (Rha, ≥99%), Fucose (Fuc, ≥99%), glucuronic acid (GlcUA, ≥99%), and galacturonic acid (GalUA, ≥97%) are all products of Sigma-Aldrich in the United States; xylose (Xyl, 98%) , glucosamine (GlcN, 98%), and galactosamine (GalN, 99%) are all products of ACROS ORGANICS in the United States; ribose (Rib, >99.0%) and arabinose (Ara, 99%) are all purchased from Xiamen Xinglong Da Biochemical Reagent Co., Ltd. Inulin (excellent grade, Belgian Orafti company). Yeast...

Embodiment 2

[0082] Example 2, Polysaccharide Ion Chromatography Fingerprint Analysis of a Sample of Broken Ganoderma Lucidum Spore Powder Sample from a Certain Origin in Jilin

[0083] 1. Extraction of spore powder polysaccharide

[0084] Accurately weigh 0.6g sample of broken Ganoderma lucidum spore powder sample from a place in Jilin, put it into a 50mL centrifuge tube, add 30mL ultrapure water, ultrasonicate in a water bath at 70°C with a power of 300W for 50min, cool down and centrifuge, wash the residue with a small amount of ultrapure water and centrifuge , the two supernatants were combined, and the combined supernatant was placed in a dialysis bag and dialyzed with ultrapure water for 12 hours, concentrated in vacuo at 55°C to nearly dryness, dissolved in ultrapure water and distilled to 5mL as a crude polysaccharide aqueous solution, to be tested;

[0085] 2. Acid-enzymatic partial hydrolysis of polysaccharides

[0086] Take 500 μL of the crude polysaccharide aqueous solution o...

Embodiment 3

[0091] Example 3, Fingerprint analysis of unbroken Ganoderma lucidum spore powder sample from a production area in Anhui

[0092] 1. Extraction of polysaccharide from broken ganoderma lucidum spore powder

[0093] Accurately weigh o.8g sample of broken Ganoderma lucidum spore powder in a 50mL centrifuge tube, add 30mL ultrapure water, ultrasonicate in a water bath at 80°C with a power of 300W for 30min, cool down and centrifuge, wash the residue with a small amount of ultrapure water and centrifuge Finally, the two supernatants were combined, and the combined supernatant was placed in a dialysis bag and dialyzed with ultrapure water for 12 hours, concentrated in vacuo at 55°C to nearly dryness, dissolved in ultrapure water and adjusted to 5mL as a crude polysaccharide aqueous solution , to be tested;

[0094] 2. Acid-enzyme partial hydrolysis of Ganoderma lucidum spore powder polysaccharide

[0095] Take 500 μL of the crude polysaccharide aqueous solution obtained in step (1...

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Abstract

The invention discloses a construction method for ion chromatography fingerprint spectrums of ganoderma lucidum spore powder polysaccharide, which comprises acid-enzyme partial hydrolysis of the ganoderma lucidum spore powder polysaccharide, the ion chromatography fingerprint analysis of monosaccharide components and oligosaccharide components of hydrolysis products of the ganoderma lucidum spore powder polysaccharide and the confirmation of standard fingerprint spectrums. By means of the high performance anion-exchange chromatography (HPAEC) analysis and the comparison of the fingerprint spectrums of polysaccharide contents in ganoderma lucidum spore powder samples from 20 different production places, the common fingerprint characteristics of the polysaccharide contents in the ganoderma lucidum spore powder samples are determined, the standard fingerprint spectrums of the monosaccharide components and the oligosaccharide components are obtained respectively, and 11 monosaccharide common characteristic peaks in monosaccharide spectrums, 4 oligosaccharide common peaks in the oligosaccharide spectrum and 1 polysaccharide peak are determined. According to the construction method for the ion chromatography fingerprint spectrums of the ganoderma lucidum spore powder polysaccharide, the method is stable, the precision degree is high, the reproducibility is good, the method is easy to master, quality conditions and production places of the ganoderma lucidum spore powder polysaccharide can be grasped from two aspects of fingerprint spectrums of the monosaccharide components and the oligosaccharide components of the acid-enzyme partial hydrolysis products which can reflect the components and structural characteristics of the ganoderma lucidum spore powder polysaccharide, and a new scientific method is provided for the quality control and the authenticity identification of the ganoderma lucidum spore powders.

Description

technical field [0001] The invention relates to a method for constructing ion chromatographic fingerprints of ganoderma lucidum spore powder polysaccharide, and belongs to the technical field of fingerprints of traditional Chinese medicine and its products, functional food raw materials and products thereof. Background technique [0002] Ganoderma lucidum is a fungus belonging to the Phylum Fungi, Class Basidiomycetes, Polyporaceae, Ganodermaceae, and the genus Ganoderma. According to Chinese Pharmacopoeia, Ganoderma lucidum (Ganoderma lucidum (Leyss.exFr) Karst.) and Zizhi (Ganoderma sinense Zhao, Xu et Zhang) can be used as medicine; Purple Mushroom and Ganoderma tsugae Murr. Ganoderma lucidum spores are extremely tiny oval germ cells ejected from the cap of Ganoderma lucidum (generally red lucidum) during the mature stage. They are called spores in biology, and they are powdery after gathering. Ganoderma lucidum spores have a variety of physiological effects such as imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 戴军朱松陈尚卫王浩豪
Owner 中食都庆(山东)生物技术有限公司
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