Detection method for fingerprint spectrum of medicinal preparation treating hepatitis

A technology of pharmaceutical preparations and detection methods, which is applied in the field of fingerprint detection, and can solve the problems of time-consuming, difficult production practice and laborious joint detection of multiple detection indicators

Inactive Publication Date: 2015-12-23
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, what the present invention is to solve is that the single detection index of the existing Yindan Pinggan Capsule cannot detect and control its quality as a whole, and the joint detection of multiple detection indexes i

Method used

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  • Detection method for fingerprint spectrum of medicinal preparation treating hepatitis
  • Detection method for fingerprint spectrum of medicinal preparation treating hepatitis
  • Detection method for fingerprint spectrum of medicinal preparation treating hepatitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The raw materials of Yindan Pinggan Capsules used in the following examples and experimental examples are as follows: capillary 350g, gentian 550g, Scutellaria baicalensis 75g, porcini powder 125g, gardenia 75g, peony root 125g, angelica 75g, licorice 125g ;

[0059] Prepare according to the following preparation method: take pig bile powder and add 3 times the amount of water to dissolve, add hydrochloric acid and heat to boil to acidify, let it cool, add water to precipitate the precipitate, add 0.8 times the amount of solid sodium hydroxide and 4.5 times the amount of water to dissolve the precipitate, Heating and saponifying for 10 hours, standing overnight, adding 5.5 times the amount of hydrochloric acid to acidify, letting cool, precipitated, filtered, washed the precipitate with water until neutral, dried, and crushed into fine powder to obtain refined pig gallbladder powder;

[0060] Take slices of Angelica sinensis, soak overnight with 4.5 times the amount of ...

Embodiment 2

[0063] The detection method of the fingerprint of the present embodiment Yindan Pinggan Capsules comprises the following steps:

[0064] (1) Take 10 g of the drug preparation to be tested, grind it finely, take 2 g, accurately weigh it, place it in a stoppered Erlenmeyer flask, add 50 mL of 50% methanol solution accurately, weigh it, heat and reflux for extraction for 30 minutes, let it cool, and then Weigh the weight, take 50% methanol solution to make up the lost weight, shake well, filter, and get the filtrate as the test solution;

[0065] (2) Accurately weigh the baicalein reference substance, add methanol to make a solution containing 0.00009g per 1mL, shake well, and use it as the reference substance solution;

[0066] (3) Chromatographic conditions: Octadecylsilane bonded silica gel is used as filler, methanol-0.1% phosphoric acid mixed solution with a volume ratio of 55:45 is used as mobile phase, the detection wavelength is 274nm, and the number of theoretical plates...

Embodiment 3

[0070] The detection method of the fingerprint of the present embodiment Yindan Pinggan Capsules comprises the following steps:

[0071] (1) Take 5 g of the pharmaceutical preparation to be tested, grind it finely, take 1 g, accurately weigh it, place it in a stoppered Erlenmeyer flask, add 40 mL of 40% methanol solution accurately, weigh it, heat and reflux for extraction for 20 minutes, let it cool, and then Weigh the weight, take 40% methanol solution to make up the lost weight, shake well, filter, and get the filtrate as the test solution;

[0072] (2) Accurately weigh the baicalein reference substance, add methanol to make a solution containing 0.00005g per 1mL, shake well, and use it as the reference substance solution;

[0073] (3) Chromatographic conditions: Octadecylsilane bonded silica gel is used as filler, methanol-0.1% phosphoric acid mixed solution with a volume ratio of 50:50 is used as mobile phase, the detection wavelength is 274nm, and the number of theoretic...

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Abstract

The invention relates to a detection method for a fingerprint spectrum of a medicinal preparation treating hepatitis. In the detection method, through optimization selection of a No.11 peak of baicalin as an internal reference peak of a fingerprint spectrum, retention time of characteristic common peaks: a No.3 peak of chlorogenic acid, a No.4 peak of gentiopicroside, a No.5 peak of paeoniflorin, a No.6 peak of liquiritin, a No.8 peak of ferulic acid and the like of a yindan pinggan capsule is determined, the yindan pinggan capsule can be detected comprehensively and rapidly, which facilitates comprehensive quality detection and whole quality control, and therefore safety and stability of medicine usage is raised.

Description

technical field [0001] The invention belongs to the field of drug analysis, in particular to a method for detecting the fingerprint of a drug preparation for treating hepatitis. Background technique [0002] Hepatitis is a common infectious disease, generally divided into icteric hepatitis and chronic hepatitis. The raw materials of Yindan Pinggan Capsules are Yinchen, Gentian, Scutellaria, Pig Gall Powder, Gardenia, Radix Paeoniae Alba, Angelica, and Licorice. Treatment of hepatitis and chronic hepatitis. Chinese patent document CN1275381A discloses the raw material composition and preparation method of Yindan Pinggan Capsules. Chinese patent document CN101987121B discloses a detection method of Yindan Pinggan Capsules, which is to measure the content of baicalin in the pharmaceutical composition for treating hepatitis by HPLC method, and also discloses the TLC identification method of Yinchen, hyodeoxychole The quality of Yindan Pinggan Capsules was controlled by the TL...

Claims

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Application Information

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IPC IPC(8): G01N30/02A61K36/744A61P1/16A61P31/20A61P31/14
Inventor 黄进明于娟陈珏
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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