50results about How to "Effectively Characterize Quality" patented technology

The invention discloses a multicomponent quantitative detection method of a lycium-rehmannia oral liquid. The establishment of the multicomponent quantitative method of the lycium-rehmannia oral liquid comprises the following steps: Step 1, preparing a test solution of the lycium-rehmannia oral liquid; Step 2, preparing a mixed reference solution; Step 3, respectively and precisely absorbing the test solution and the mixed reference solution and injecting into a liquid chromatogram, and recording a chromatogram; and Step 4, calculating contents of components in a sample through the chromatogram of the mixed reference substance and the lycium-rehmannia oral liquid according to retention time and peak area. Through the multicomponent quantitative analysis method of the lycium-rehmannia oralliquid, quality of the lycium-rehmannia oral liquid can be represented comprehensively and objectively. The multicomponent quantitative detection method provided by the invention has advantages of simple and stable method, high precision, good repeatability and the like.

The establishment of the fingerprint spectrum of the Xiasangju preparation and the fingerprint spectrum relate to the quality control method of the traditional Chinese medicine preparation, specifically the method for establishing the HPLC fingerprint spectrum to detect the alcohol extract in the Xiasangju preparation. The method comprises: (a) preparation of the reference substance solution; (b) preparation of the test solution; (c) chromatographic conditions: the chromatographic column is filled with octadecylsilane bonded silica gel; gradient elution is adopted, and the mobile phase is Gradient eluent composed of 0.1-3% acetic acid and methanol; column temperature: 25-50°C; UV detection wavelength: 285-295nm; flow rate: 0.5-1.5mL min-1; time: 30-80min; (d) Determination: fingerprints obtained by high performance liquid chromatography. The beneficial effect of the present invention: it can effectively characterize the quality of the Xiasangju preparation, which is beneficial to monitor the quality of the product; the method is simple, stable, high in precision and good in reproducibility; it can quickly and accurately identify the authenticity of the product.

The invention relates to a method for establishing a characteristic fingerprint of Dendrobium, and the steps are as follows: 1. Preparation of standard sample solution: taking Dendrobium, pulverizing, sieving, adding methanol to ultrasonic, filtering, filtrate was concentrated to dryness by rotary evaporation, dissolved in methanol solvent, and finally determined (2) Preparation of reference substance solution: Precisely weigh a certain amount of isoschavtoside, dissolve and dilute it with methanol to prepare a reference substance solution; (3) Determination of standard fingerprint: put the standard sample solution into The solution and the reference solution were injected into the high-performance liquid chromatograph respectively, and the chromatographic peak of schaffertoside was used as the reference peak of the fingerprint to calculate the relative retention time and retention peak area of ​​each characteristic peak of the standard sample, and to formulate the standard fingerprint. In the method for constructing a fingerprint spectrum, the invention qualitatively characterizes the fingerprint characteristic peaks with a relative peak area greater than 5%, breaks through the excessive ambiguity of the previous fingerprint spectrum, further clarifies the main components of the fingerprint spectrum, and improves the integrity of the Dendrobium fingerprint spectrum. and ingredient specificity.