50results about How to "Effectively Characterize Quality" patented technology

The invention discloses a method for detecting a fingerprint spectrum of Guigui zhugan decoction. The fingerprint spectrum is established by the following steps: S1, preparing a Guigui zhugan decoction test sample solution; S2, preparing a reference solution; S3, absorbing the test sample solution and the reference solution precisely and respectively and injecting the solutions into a liquid chromatograph, and recording a chromatogram map; and S4, outputting a Guigui zhugan decoction fingerprint spectrum obtained in the S3 into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks exiting in all chromatogram maps of Guigui zhugan decoction with different batches as common peaks and generating a contrast fingerprint spectrumof the Guigui zhugan decoction based on an average value calculation method, and calculating relative retention time and relative peak areas of all common peaks. According to the invention, the detecting method having advantages of high stability and precision, and high reproducibility and the like is capable of analyzing the effective components in the Guigui zhugan decoction accurately and hasthe important significance in evaluating the quality of the Guigui zhugan decoction comprehensively and objectively.

The present invention discloses a Shenqi hypoglycemic preparation HPLC standard finger print and a construction method thereof. The construction method comprises the following steps: preparing test sample solutions and a reference solution, measuring the test sample solutions and the reference solution by using the HPLC method and the linear gradient elution process, so as to obtain the Shenqi hypoglycemic preparation finger prints, wherein the chromatographic condition is that a mobile phase A is acetonitrile, a mobile phase B is 0.1% (v/v) phosphoric acid solution, the detection wavelength is 203nm, the column temperature is 25-35 DEG C and the flow rate is 0.8-1.2mL/min. The common characteristic peaks of at least ten finger prints are used as the characteristic peaks of the standard finger print; the standard finger print comprises 38 characteristic peaks, and the numbers of the characteristic peaks of saponin of ginseng stem and leaf, astragalus, Chinese magnoliaving, rehmannia root and raspberry are 13, 4, 7, 3 and 4 respectively. The Shenqi hypoglycemic preparation HPLC standard finger print and the construction method thereof can comprehensively and accurately evaluate the whole quality of the Shenqi hypoglycemic preparation, and facilitate to ensure the quality and the clinical curative effect of the Shenqi hypoglycemic preparation.

The related detection method for amino acid content in Xiasangju preparation comprises: (a) preparing reference solution; (b) preparing sample solution; (c) chromatogram condition: using octadecylsilicane chemically bonded silica as filler, using the gradient elution liquid with 0.05~0.10mol/L NaAc solution buffer and 20-80% acetonitrile solution as mobile phase with pH value as 4.50-5.05, 30-50Deg temperature, activation wavelength 250nm, emission wavelength 395nm, flow rate as 0.5-1.5mL .min-1, and 30-80min; (d) obtaining the fingerprint spectrum with HPLC. This invention is simple and stable, and has well repeatability and precision.

This invention relate to the found of summer mulberry chrysanthemum preparation finger print, refer methodical control of quality of Chinese traditional medicine preparation. The invention is a method of founding HPLC finger print to detect alcohol extracted thing in summer mulberry chrysanthemum preparation. The method include;(a) preparation of control article solution;(b) preparation of examining solution; (c) chromatographic condition; chromatographic column take octadecyl silane bonded silica gel as padding;adopt gradient elution, mobile phase is gradient elution liquid composed by 0.1 to 3 percent acetic acid and methanol; column temperature;25 to 50 deg; ultraviolet detecting wavelength is 285 to 295 nm;velocity of flow;0.5 to 1.5 ml per min; time;30 to 80 min;(d) measuring; high efficiency liquid chromatography to gain finger print. This invention can validly characterize quality of summer mulberry chrysanthemum preparation, in favor of monitoring product quality; possess merit of method handiness, stable, high degree of precision, good reappearance; the invention can quickly and exactly discriminate the product.

The invention provides an HPLC fingerprint spectrum determination method for cannabis sativa. The method is characterized by comprising the steps of (1) preparing a test solution: taking the cannabissativa medicinal material, crushing, sieving and baking the cannabis sativa medicinal material, precisely weighing baked cannabis sativa medicinal material powder, adding an extracting agent, carryingout ultrasonic extraction, carrying out standing until the room temperature is reached, passing through a microporous filter membrane, and taking subsequent filtrate; (2) preparing a reference solution: taking cannabidiolic acid, cannabidivarin, cannabigerol, cannabidiol, delta 9-tetrahydrocannabinol, cannabichromene, tetrahydrocannabinol and geranylflavone A reference substances, and adding methanol to dissolve the reference substances so as to prepare the reference substance solution; and (3) performing determination: precisely sucking the reference solution and the test solution into an HPLC, performing determination, and recording a chromatogram.

The invention is related to method for controlling quality of traditional Chinese medicinal materials and productions. Comparing fingerprints of high performance liquid chromatography HPLC for ten batches of ganoderma lucidum spore, oil of ganoderma lucidum spore determines mutual characters so as to obtain standard fingerprints. There are 15 mutual peaks in fingerprints. There are 4 peaks with its area exceeding 5% of total peak area. Features of the invention are: simple, stable, high accuracy, good reproducibility, easy of mastering. The invention discloses total new method for controlling quality of ganoderma lucidum spore, and oil of ganoderma lucidum spore, and distinguishing true or false products.

The invention discloses a high performance liquid chromatography (HPLC) standard fingerprint spectrum of a lucid ganoderma capsule preparation and a construction method and application of the standard fingerprint spectrum. The construction method comprises the following steps: preparing a test solution and a reference substance solution, and carrying out linear gradient elution, wherein a mobile phase A is acetonitrile, a mobile phase B is 0.05-0.15 percent (v/v) phosphoric acid aqueous solution, the detection wavelength is 252nm, the column temperature is 25-35 DEG C, the flow velocity is 1.0mL/min; measuring through HPLC, obtaining a lucid ganoderma capsule fingerprint spectrum, and taking a common characteristic peak of at least 14 batches of fingerprint spectrums as the characteristic peak of the standard fingerprint spectrum, wherein the standard fingerprint spectrum totally has 40 characteristic peaks, and the numbers of characteristic peaks belonging to lucid ganoderma, bighead atractylodes rhizome, rhizoma polygonati, the seed of Chinese dodder, schisandra chinensis, liquorice and the fruit of Chinese wolfberry are respectively 20, 3, 3, 5, 1, 5 and 2. According to the method and the standard fingerprint spectrum, the overall quality of the lucid ganoderma preparation can be comprehensively and accurately evaluated, and the quality and drug effect of the lucid ganoderma preparation are guaranteed.

The invention relates to a method for building a fingerprint of loquat leaf medicinal materials and a fingerprint thereof. The method comprises the following steps of: firstly collecting the loquat leaf medicinal materials for over 10 batches, preparing test sample solution and a reference product solution, implementing measurement by referring to and applying a high-efficiency liquid chromatography method and a high-efficiency capillary electrophoresis method, and finally implementing analysis by applying a computation software of the similarity between the fingerprint of traditional Chinese medicines (which is recommended by the State Pharmacopoeia Committee). The method of the invention has the advantages of simplicity, stability, high precision, good reproducibility and easy grasp, can grasp the variety and the quality condition of the loquat leaves from the integral characteristic face and the high profile of chromatogram, and can be used as one of the methods for evaluating the quality of the loquat leaves and distinguishing the loquat leaves between true and false.

The invention discloses a glossy privet fruit fingerprint spectrum detection method. Building of glossy privet fruit fingerprint spectrums includes the following steps that 1, a test solution is prepared; 2, a mixed comparison product solution is prepared; 3, the test solution are accurately sucked to be injected into a liquid chromatograph, and chromatogram maps are recorded; and 4, the glossy privet fruit fingerprint spectrums obtained in the step 3 are subjected to instrument guiding-out, a traditional Chinese medicine chromatography fingerprint spectrum similarity evaluation system is guided in, chromatographic peaks existing in the chromatography maps of glossy privet fruits in different batches are selected as common peaks; comparison fingerprint spectrums of the glossy privet fruitsare generated by an average calculation method; and the relative retention time and the relative peak area of all common peaks are calculated. By means of glossy privet fruit fingerprint spectrums, the quality of the glossy privet fruits can be comprehensively and objectively represented. The fingerprint spectrum detection method has the advantages of the simple method, stability, high precision,good reproducibility and the like.

The invention relates to a construction method and a quality detection method of a UPLC feature map of a Hangzhou chrysanthemum medicinal material. The construction method comprises the following steps: sample solution preparation; taking the Hangzhou chrysanthemum medicinal material, crushing, adding a solvent for extraction and filtering, wherein a filtrate is taken as a sample solution; reference solution preparation: taking chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, galuteolin and linarin and adding a solvent for dissolution, wherein an obtained solution isa reference solution; ultra performance liquid chromatography detection: absorbing the sample solution and the reference solution, injecting into a liquid chromatograph and detecting to obtain the UPLC feature map of the Hangzhou chrysanthemum medicinal material. The UPLC feature map is rich in characteristic peak information, can fully reflect the chemical composition of the Hangzhou chrysanthemum medicinal material and can also effectively characterize the qualities of a Hangzhou chrysanthemum decoction piece and standard decoction and identify the product authenticity.

The invention discloses a detection method for the fingerprint of a radix puerariae assorted Chinese herbal tea. The detection method comprises the following steps: 1, preparing a radix puerariae assorted Chinese herbal tea test solution; 2, preparing a mixed reference substance solution; 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording chromatogram maps; and 4, exporting the radix puerariae assorted Chinese herbal tea fingerprint, importing the fingerprint into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks existing in chromatograms of different batches of radix puerariae assorted Chinese herbal tea as common peaks, generating a contrast fingerprint spectrum of the radix puerariae assorted Chinese herbal tea by using an average value calculation method, calculating the relative retention time and the relative peak area of each common peak, comparing the fingerprint of the radix puerariae assorted Chinese herbal tea with the spectrum of the mixed standard substance, and identifying main component peaks. The radix puerariae assorted Chinese herbal tea fingerprint provided by the invention can comprehensively and objectively characterize the quality of the radix puerariae assorted Chinese herbal tea. And the detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.

The invention discloses a detection method for the fingerprint spectrum of an instant spina date seed heart calming solid beverage. The method comprises the following steps of: step 1, preparing a test solution; step 2, preparing a mixed reference substance solution; step 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording chromatograms; step 4, exporting the fingerprint spectrum of the test solution from an instrument, importing the fingerprint spectrum into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, and selecting chromatographic peaks existing in chromatograms of different batches of instant spina date seed heart-calming drink solid beverages as common peaks; generating a control fingerprint spectrum of the instant spina date heart-calming drink solid beverages by using an average value calculation method; calculating the relative retention time and the relative peak area of each common peak; and comparing with the spectrum with the spectrum of the mixed standard product, and identifying main component peaks. The fingerprint spectrum can comprehensively and objectively characterize the quality of the instant spina date seed heart-calming drink solid beverage. The detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.