50results about How to "Effectively Characterize Quality" patented technology

The invention discloses an establishment of a mesona blume medicinal material finger print, and a finger print thereof, and relates to a quality control method for traditional Chinese medicinal materials, specifically relates to a method for detecting an extracting solution of the mesona blume medicinal material through establishing HPLC finger print. The method comprises the following steps: preparing a reference substance solution; preparing a tested sample solution; adopting octadecylsilane chemically bonded silica gel as a filling for a chromatographic column; adopting gradient elution, wherein a mobile phase is a gradient eluent comprising 1.0-3.0% of formic acid and acetonitrile, a column temperature is 20-40 DEG C, an ultraviolet detection wavelength is 300-360 nm, a detection time is 90-120 minutes; carrying out high performance liquid chromatography to detect the finger print. According to the present invention, the quality of the mesona blume medicinal material can be effectively characterized so as to monitor the quality of the medicinal material; the method has characteristics of simplicity, stability, high precision, good reproducibility, and the authenticity of the medicinal material can be quickly and accurately identified.

The invention discloses a method for detecting fingerprints of Mingge Shuqing Drink, the method comprising the following steps: step 1, preparation of Mingge Shuqing drink test solution; step 2, preparation of a mixed reference solution; step 3, precise absorption of the mixed control The product solution and the test solution are injected into the liquid chromatograph, and the chromatograms are recorded; step 4, the Ming Ge Shu Qing drink fingerprint instrument obtained in step 3 is exported, and imported into the similarity evaluation system of traditional Chinese medicine chromatographic fingerprints, and different batches of fingerprints are selected. The chromatographic peaks that all exist in the chromatogram of Geshu Qingyin are used as common peaks; the control fingerprint of Minggeshu Qingyin is generated by the average value calculation method; the fingerprint of Minggeshu Qingyin obtained in step 5 and step 3 is compared with the mixed standard spectrum , to identify the main components. The fingerprint of Minggeshu Qingyin provided by the invention can comprehensively and objectively characterize the quality of Minggeshuqingyin; and has the advantages of simple, stable, high precision, good reproducibility and the like.

The invention discloses a multicomponent quantitative detection method of a lycium-rehmannia oral liquid. The establishment of the multicomponent quantitative method of the lycium-rehmannia oral liquid comprises the following steps: Step 1, preparing a test solution of the lycium-rehmannia oral liquid; Step 2, preparing a mixed reference solution; Step 3, respectively and precisely absorbing the test solution and the mixed reference solution and injecting into a liquid chromatogram, and recording a chromatogram; and Step 4, calculating contents of components in a sample through the chromatogram of the mixed reference substance and the lycium-rehmannia oral liquid according to retention time and peak area. Through the multicomponent quantitative analysis method of the lycium-rehmannia oralliquid, quality of the lycium-rehmannia oral liquid can be represented comprehensively and objectively. The multicomponent quantitative detection method provided by the invention has advantages of simple and stable method, high precision, good repeatability and the like.

The establishment of the fingerprint spectrum of the Xiasangju preparation and the fingerprint spectrum relate to the quality control method of the traditional Chinese medicine preparation, specifically the method for establishing the HPLC fingerprint spectrum to detect the alcohol extract in the Xiasangju preparation. The method comprises: (a) preparation of the reference substance solution; (b) preparation of the test solution; (c) chromatographic conditions: the chromatographic column is filled with octadecylsilane bonded silica gel; gradient elution is adopted, and the mobile phase is Gradient eluent composed of 0.1-3% acetic acid and methanol; column temperature: 25-50°C; UV detection wavelength: 285-295nm; flow rate: 0.5-1.5mL min-1; time: 30-80min; (d) Determination: fingerprints obtained by high performance liquid chromatography. The beneficial effect of the present invention: it can effectively characterize the quality of the Xiasangju preparation, which is beneficial to monitor the quality of the product; the method is simple, stable, high in precision and good in reproducibility; it can quickly and accurately identify the authenticity of the product.

The invention discloses a method for detecting the fingerprint of anti-lung cancer sea mulberry recipe, comprising the following steps: step 1: preparation of a reference peak solution; step 2: preparation of a test solution of anti-lung cancer ha mu recipe; step 3: separately precise Inject the quantitative reference peak solution and the test solution into the liquid chromatograph, and record the chromatogram; Step 4: Export the Haimu Fang fingerprint instrument obtained in step 3, and import it into the Chinese medicine chromatographic fingerprint similarity evaluation system, select different The chromatographic peaks that exist in the chromatograms of the batches of Haimufang tested are regarded as common peaks, and the average value calculation method is used to generate the control fingerprint of Haimufang, and the relative retention time and relative peak area of ​​each common peak are calculated. The fingerprint spectrum of the Haimu prescription provided by the invention can comprehensively and objectively characterize the quality of the Haimu prescription, and is beneficial to comprehensively monitor the quality of the medicine. The fingerprint detection method provided by the invention has the advantages of simple method, stability, high precision, good reproducibility and the like.

The invention discloses a method for detecting the fingerprint of Xiaoer Ganmaoshu Granules. The method comprises the following steps: step 1, preparation of the test solution of Xiaoer Ganmaoshu Granules; step 2, preparation of a single-standard reference substance solution; step 3, precision Draw the test solution into the liquid chromatograph, and record the chromatogram; step 4, export the fingerprint instrument of Xiaoermaoshu granules obtained in step 3, and import it into the similarity evaluation system of Chinese medicine chromatographic fingerprints, and select different batches of Xiaoermaoshu The chromatographic peaks that exist in the chromatograms of Shu granules are taken as common peaks; the control fingerprint of Xiaoermaoshu granules is generated by the average value calculation method; the relative retention time and relative peak area of ​​each common peak are calculated. The fingerprint of Xiaoer Ganmaoshu granules provided by the present invention can comprehensively and objectively characterize the quality of Xiaoer Ganmaoshu granules. The fingerprint detection method provided by the invention has the advantages of simplicity, stability, high precision, good reproducibility and the like.

The invention relates to a method for establishing a characteristic fingerprint of Dendrobium, and the steps are as follows: 1. Preparation of standard sample solution: taking Dendrobium, pulverizing, sieving, adding methanol to ultrasonic, filtering, filtrate was concentrated to dryness by rotary evaporation, dissolved in methanol solvent, and finally determined (2) Preparation of reference substance solution: Precisely weigh a certain amount of isoschavtoside, dissolve and dilute it with methanol to prepare a reference substance solution; (3) Determination of standard fingerprint: put the standard sample solution into The solution and the reference solution were injected into the high-performance liquid chromatograph respectively, and the chromatographic peak of schaffertoside was used as the reference peak of the fingerprint to calculate the relative retention time and retention peak area of ​​each characteristic peak of the standard sample, and to formulate the standard fingerprint. In the method for constructing a fingerprint spectrum, the invention qualitatively characterizes the fingerprint characteristic peaks with a relative peak area greater than 5%, breaks through the excessive ambiguity of the previous fingerprint spectrum, further clarifies the main components of the fingerprint spectrum, and improves the integrity of the Dendrobium fingerprint spectrum. and ingredient specificity.

The invention discloses a finger-print quality determination method on wuji powder preparation and intermediate thereof. The finger-print quality determination method includes the following steps: quantitative wuji powder preparation or intermediate thereof is weighed, at least one of solvents of water, methyl alcohol or ethyl alcohol is used for extraction, constant volume is carried out, so as to obtain test solution; the test solution is sucked and injected into a liquid chromatograph, and high efficiency liquid chromatography and gradient elution are adopted for carrying out determination, so as to obtain the finger-print of wuji powder preparation or intermediate thereof; wherein the chromatographic conditions of the high efficiency liquid chromatography include that the filler of chromatographic column is octadecylsilane chemically bonded silica, mobile phase A is methyl alcohol, mobile phase B is phosphoric acid aqueous solution, the volume ratio of the mobile phase A and the mobile phase B is gradually increased along with time, and detection wavelength is 285-305nm. The invention has the characteristics that method is simple and stable, has high precision and good reproducibility and is easy to grasp.