Glossy privet fruit fingerprint spectrum detection method

A fingerprint and detection method technology, applied in the field of traditional Chinese medicine detection, can solve problems such as the inability to reflect the integrity of traditional Chinese medicine, and achieve the effect of simple method, high precision and good reproducibility

Active Publication Date: 2019-04-16
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the quality control method of traditional Chinese medicine only analyzes a few of the known components, which cannot reflect the overall characteristics of traditional Chinese medicine. It is necessary to d

Method used

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  • Glossy privet fruit fingerprint spectrum detection method
  • Glossy privet fruit fingerprint spectrum detection method
  • Glossy privet fruit fingerprint spectrum detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Instruments used in the following examples

[0041]Shimadzu LC-20AB high performance liquid chromatography system from Shimadzu, Japan, including online degasser, automatic sampler Prominence SIL-20A, diode array detector SPD-M20A and column thermostat CTO-20A; ME204E electronic analytical balance (Mei Teller-Toledo International Co., Ltd., accurate to 1 / 100,000); BSM220.4 analytical balance (Shanghai Zhuojing Company, accurate to 1 / 10,000); GVS ultrasonic cleaner (Shenzhen Gowell Technology Co., Ltd. Company); TGL-16C high-speed desktop centrifuge (Shanghai Anting Scientific Instrument Factory).

[0042] The reagents used in the following examples are as follows: privetin analysis standard substance (lot number 5937, purity 99.5%), salidroside reference substance (lot number 4256, purity 99.7%), tyrosol reference substance (lot number 1487, purity 99.7%) All were purchased from Shanghai Shidande Standard Technology Service Co., Ltd. Reference substances of rutin, que...

Embodiment 2

[0061] The methodological research of embodiment 2 fingerprint spectrum detection:

[0062] 1. Methodological investigation

[0063] 1.1 Inspection of precision

[0064] Get the wine Ligustrum lucidum with batch number 180509, according to the preparation method of the test sample in the above-mentioned embodiment 1, prepare the test sample solution, continuously sample 6 times, each sample injection volume is 10 μ L, detect according to the chromatographic conditions of the above-mentioned embodiment 1 , measure its HPLC chromatogram, investigate 10 common fingerprint peaks in the chromatogram, the results show that the retention time RSD of common fingerprint peaks is all less than 2.5%, and the common peak area RSD is all less than 2.6%, indicating that the precision of the instrument is better .

[0065] 1.2 Stability investigation

[0066] Get batch number and be the wine Ligustrum lucidum 180509, prepare need testing solution according to the need testing sample prepa...

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Abstract

The invention discloses a glossy privet fruit fingerprint spectrum detection method. Building of glossy privet fruit fingerprint spectrums includes the following steps that 1, a test solution is prepared; 2, a mixed comparison product solution is prepared; 3, the test solution are accurately sucked to be injected into a liquid chromatograph, and chromatogram maps are recorded; and 4, the glossy privet fruit fingerprint spectrums obtained in the step 3 are subjected to instrument guiding-out, a traditional Chinese medicine chromatography fingerprint spectrum similarity evaluation system is guided in, chromatographic peaks existing in the chromatography maps of glossy privet fruits in different batches are selected as common peaks; comparison fingerprint spectrums of the glossy privet fruitsare generated by an average calculation method; and the relative retention time and the relative peak area of all common peaks are calculated. By means of glossy privet fruit fingerprint spectrums, the quality of the glossy privet fruits can be comprehensively and objectively represented. The fingerprint spectrum detection method has the advantages of the simple method, stability, high precision,good reproducibility and the like.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine detection, and in particular relates to a fingerprint detection method of Ligustrum lucidum. Background technique [0002] Ligustrum lucidum Ait. is the dry ripe fruit of Ligustrum lucidum Ait. Its nature is sweet, bitter, cool. Return liver, kidney channel. It has the effect of nourishing the liver and kidney, improving eyesight and black hair. For liver and kidney yin deficiency, dizziness and tinnitus, soreness of the waist and knees, premature graying of the beard and hair, dark eyes, internal heat and quenching thirst, bone steaming and hot flashes. The wine steaming method is a conventional processing method of Ligustrum lucidum, and Ligustrum lucidum wine products are more effective in nourishing the liver and kidney than raw products. [0003] With the widespread application of traditional Chinese medicine, its effectiveness and safety issues have attracted widespr...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 蔡宝昌李国维金俊杰秦昆明陈海超张金龙孙戡平
Owner NANJING HAICHANG CHINESE MEDICINE GRPCO LTD
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