94results about How to "Reduce the possibility of human handling" patented technology

The invention discloses a method for detecting a fingerprint spectrum of Guigui zhugan decoction. The fingerprint spectrum is established by the following steps: S1, preparing a Guigui zhugan decoction test sample solution; S2, preparing a reference solution; S3, absorbing the test sample solution and the reference solution precisely and respectively and injecting the solutions into a liquid chromatograph, and recording a chromatogram map; and S4, outputting a Guigui zhugan decoction fingerprint spectrum obtained in the S3 into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks exiting in all chromatogram maps of Guigui zhugan decoction with different batches as common peaks and generating a contrast fingerprint spectrumof the Guigui zhugan decoction based on an average value calculation method, and calculating relative retention time and relative peak areas of all common peaks. According to the invention, the detecting method having advantages of high stability and precision, and high reproducibility and the like is capable of analyzing the effective components in the Guigui zhugan decoction accurately and hasthe important significance in evaluating the quality of the Guigui zhugan decoction comprehensively and objectively.

The invention provides a constructing method of an electrospray ionization mass spectrometry fingerprint of Guangdong herbal tea granules and a standard fingerprint, and relates to a quality control method of a traditional Chinese medicine preparation, in particular to a method for establishing the electrospray ionization mass spectrometry fingerprint to achieve quality evaluation and source distinguishment of the Guangdong herbal tea granules. The method comprises the following steps: (1) preparation of a test solution; (2) mass spectrometry condition: electrospray ionization mass spectrometry is directly used for detection, mass spectrometry contour map is recorded in a full-scanning mode, and scanning quality range m/z is 100-1000; (3) detection: the fingerprint is directly obtained by the electrospray ionization mass spectrometry; (4) standard fingerprint: the fingerprint of a test product uses the fingerprint according to the Similarity Evaluation System for Chromatographic Fingerprint of TCM(2004 A edition); and the standard fingerprint is generated through calculation using a mean value method; and (5) main ingredient analysis: main ingredients of the fingerprint of the test product are analyzed by using statistics software, so as to evaluate the quality and distinguish sources. The constructing method of an electrospray ionization mass spectrometry fingerprint of the Guangdong herbal tea granules, provided by the invention, is beneficial for monitoring product quality and has the advantages as follows: the quality of the Guangdong herbal tea granules is rapidly, efficiently and reliably represented; simple and convenient method, stability, high sensitivity and good repetitiveness are achieved; requirements on high throughput analysis are satisfied; and the quality of a product can be rapidly and accurately identified.

The invention discloses a method for establishing fingerprint of Deng's herbal tea granules and standard fingerprint thereof, and relates to a method for controlling quality of Chinese medicine preparation, in particular to a method for establishing HPLC (high performance liquid chromatography) standard fingerprint to detect quality of the Deng's herbal tea granules. The method comprises the following steps: (1) preparation of reference substance solution; (2) preparation of test substance solution; (3) chromatographic conditions: the chromatographic column takes octadecylsilane chemically bonded silica as filler; gradient elution is adopted, wherein the mobile phase of gradient eluent is 0.1-3%, and the gradient eluent consists of phosphoric acid and acetonitrile; the column temperature is 20-50 DEG C; the wavelength for ultraviolet detection is 240-262 nm; the flow velocity is 0.3-1.0 mL/min; and the time is 20-60 minutes; and (4) measurement: the fingerprint is obtained by HPLC. The method disclosed by the invention effectively represents the quality of Deng's herbal tea granules, is favorable for the monitoring of product quality, has the advantages of simplicity, stability, high precision and good reproducibility, and can quickly and accurately identify the true and false as well as the quality of the product.

The invention relates to a fingerprint spectrum detection method for meridian warming decoction. The method comprises the following steps: 1) preparing a test solution; weighing 1-2g of angelica sinensis, 1-2g of ligusticum wallichii, 1-2g of radix paeoniae alba, 1-2g of cinnamon, 1-2g of moutan bark, 1-2g of curcuma zedoary powder, weighing 3-4g of ginseng powder, 3-4g of liquorice powder and 3-4g of radix achyranthis bidentatae powder; adding 250-350mL of water, uniformly mixing, heating to boil with strong fire, slowly decocting with slow fire, filtering while the decoction is about 150-170mL, and adding water into the filtrate to dilute to 240-260mL; precisely sucking 8-12mL of the standard decoction of the meridian warming decoction, adding methanol to dilute to 40-60mL, sealing, weighing the mass, carrying out ultrasonic (the power is 180-220W and the frequency is 40-60kHz) treatment for 8-12min, standing overnight, weighing the mass again, supplementing the lost mass with methanol, uniformly shaking, filtering, and taking the subsequent filtrate, thereby obtaining the test solution; 2) detecting: taking and injecting 5-15 [mu]L of the test solution obtained in the previous step into a high performance liquid chromatograph, and obtaining a chromatogram; and 3) performing result judgment: comparing the chromatogram obtained in the previous step with a standard control fingerprint, and if the similarity is greater than 90%, the sample being qualified.

The invention discloses an establishing method of a fingerprint spectrum of honeysuckle-fructus forsythiae heat-clearing tablets and the fingerprint spectrum. The establishing method of the fingerprint spectrum of the honeysuckle-fructus forsythiae heat-clearing tablets includes the following steps: step 1, preparing a test sample solution of the honeysuckle-fructus forsythiae heat-clearing tablets; step 2, respectively precisely sucking the test sample solution and injecting into liquid chromatographs, and recording a chromatogram; step 3, exporting the honeysuckle-fructus forsythiae heat-clearing tablet fingerprint spectrum obtained in the step 2 from the instrument, and importing into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system; selecting chromatographic peaks existing in chromatograms of different batches of the honeysuckle-fructus forsythiae heat-clearing tablets as common peaks; generating a reference fingerprint spectrum of the honeysuckle-fructus forsythiae heat-clearing tablets by an average value calculation method; and calculating the relative retention time and the relative peak area of each common peak. The honeysuckle-fructus forsythiae heat-clearing tablet fingerprint spectrum established by the method provided by the invention can effectively characterize the quality of the honeysuckle-fructus forsythiae heat-clearing tablets, and is conducive to comprehensive supervisory control of the drug quality. The method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like.

The invention provides a high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) fingerprint detection method for a main saponin component of a compound wintercreeper preparation (including mixture, capsules and tablets). The fingerprint detection method of the invention comprises the steps of: preparing test solution and measuring the fingerprint under a certain chromatographic condition. Scientific tests prove that the fingerprint measured by the method has high repeatability, so that the method can be used as a method for checking and identifying the main saponin component of the compound wintercreeper preparation and can also be used for measuring astragaloside content and ginsenoside Rb1 content of the preparation.

This invention relate to the found of summer mulberry chrysanthemum preparation finger print, refer methodical control of quality of Chinese traditional medicine preparation. The invention is a method of founding HPLC finger print to detect alcohol extracted thing in summer mulberry chrysanthemum preparation. The method include;(a) preparation of control article solution;(b) preparation of examining solution; (c) chromatographic condition; chromatographic column take octadecyl silane bonded silica gel as padding;adopt gradient elution, mobile phase is gradient elution liquid composed by 0.1 to 3 percent acetic acid and methanol; column temperature;25 to 50 deg; ultraviolet detecting wavelength is 285 to 295 nm;velocity of flow;0.5 to 1.5 ml per min; time;30 to 80 min;(d) measuring; high efficiency liquid chromatography to gain finger print. This invention can validly characterize quality of summer mulberry chrysanthemum preparation, in favor of monitoring product quality; possess merit of method handiness, stable, high degree of precision, good reappearance; the invention can quickly and exactly discriminate the product.

The invention provides an HPLC fingerprint spectrum determination method for cannabis sativa. The method is characterized by comprising the steps of (1) preparing a test solution: taking the cannabissativa medicinal material, crushing, sieving and baking the cannabis sativa medicinal material, precisely weighing baked cannabis sativa medicinal material powder, adding an extracting agent, carryingout ultrasonic extraction, carrying out standing until the room temperature is reached, passing through a microporous filter membrane, and taking subsequent filtrate; (2) preparing a reference solution: taking cannabidiolic acid, cannabidivarin, cannabigerol, cannabidiol, delta 9-tetrahydrocannabinol, cannabichromene, tetrahydrocannabinol and geranylflavone A reference substances, and adding methanol to dissolve the reference substances so as to prepare the reference substance solution; and (3) performing determination: precisely sucking the reference solution and the test solution into an HPLC, performing determination, and recording a chromatogram.

The invention provides a paper-based electrospray ionization mass spectrometry fingerprint technology and a construction method thereof. The paper-based electrospray ionization mass spectrometry fingerprint technolog comprises the steps of (1) manufacturing a paper-based carrier; (2) performing a paper-based eelctrospray test: aligning the tip end of the paper-based carrier to a mass spectrometry entrance, dropping a sample at the center position, loading a high-voltage electric field, dropping a spray solvent for dissolving active components in the sample, moving to the tip end of the paper-based carrier under the action of the high-voltage electric field to form a Taylor cone at the tip end, generating charged spray liquid drops, ionizing the active components and performing the mass spectrometry; (3) recording the mass spectrometry; (4) obtaining a fingerprint; (5) obtaining a standard fingerprint: calculating the standard fingerprint by an averaging method; (6) analyzing main components: performing quality evaluation, true and false identification and source distinguishing through main component analysis. The invention provides a brand-new high-flux mass spectrometry fingerprint analysis method which does not comprise sample pretreatment and chromatographic separation, thus quality monitoring for liquid Chinese herbal preparations or an extracting solution sample is facilitated.

The invention discloses a glossy privet fruit fingerprint spectrum detection method. Building of glossy privet fruit fingerprint spectrums includes the following steps that 1, a test solution is prepared; 2, a mixed comparison product solution is prepared; 3, the test solution are accurately sucked to be injected into a liquid chromatograph, and chromatogram maps are recorded; and 4, the glossy privet fruit fingerprint spectrums obtained in the step 3 are subjected to instrument guiding-out, a traditional Chinese medicine chromatography fingerprint spectrum similarity evaluation system is guided in, chromatographic peaks existing in the chromatography maps of glossy privet fruits in different batches are selected as common peaks; comparison fingerprint spectrums of the glossy privet fruitsare generated by an average calculation method; and the relative retention time and the relative peak area of all common peaks are calculated. By means of glossy privet fruit fingerprint spectrums, the quality of the glossy privet fruits can be comprehensively and objectively represented. The fingerprint spectrum detection method has the advantages of the simple method, stability, high precision,good reproducibility and the like.

The present invention provides establishment of a pigeonpea leaf herb finger print, and a finger print thereof, and relates to a quality control method for traditional Chinese medicine herbs, specifically to a method for establishing a HPLC finger print to detect a pigeonpea leaf herb extraction solution. The method comprises: (a) preparing a reference substance solution; (b) preparing a sample solution; (c) adopting the following chromatograph conditions, wherein a chromatographic column adopts octadecylsilane chemically bonded silica gel as a filler, gradient elution is performed, a mobile phase is a gradient elution solution comprising methanol and 1.0-3.0% glacial acetic acid, a column temperature is 20-40 DEG C, an ultraviolet detection wavelength is 220-300 nm, a flow rate is 0.5-1.2 mL.min<-1>, and a time is 100-140 min; and (d) determining, wherein a high performance liquid chromatography method is performed to determine the finger print. According to the present invention, quality of the pigeonpea leaf herb can be effectively characterized, herb quality monitoring can be easily achieved, the method has characteristics of simpleness, stability, high precision and good reproducibility, and authenticity of the herb can be quickly and accurately identified.

The invention relates to a construction method and a quality detection method of a UPLC feature map of a Hangzhou chrysanthemum medicinal material. The construction method comprises the following steps: sample solution preparation; taking the Hangzhou chrysanthemum medicinal material, crushing, adding a solvent for extraction and filtering, wherein a filtrate is taken as a sample solution; reference solution preparation: taking chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, galuteolin and linarin and adding a solvent for dissolution, wherein an obtained solution isa reference solution; ultra performance liquid chromatography detection: absorbing the sample solution and the reference solution, injecting into a liquid chromatograph and detecting to obtain the UPLC feature map of the Hangzhou chrysanthemum medicinal material. The UPLC feature map is rich in characteristic peak information, can fully reflect the chemical composition of the Hangzhou chrysanthemum medicinal material and can also effectively characterize the qualities of a Hangzhou chrysanthemum decoction piece and standard decoction and identify the product authenticity.

The invention relates to a marsdenia tenacissima medicinal material, a method for establishing fingerprints of a marsdenia tenacissima medicinal material preparation, application of the method to quality control of the marsdenia tenacissima medicinal material and the preparation of marsdenia tenacissima medicinal material, and discloses fingerprints of phenolic acids and steroid saponins in the medicinal material and the preparation of marsdenia tenacissima, and a measurement method and an application of the fingerprints. The quality of the medicinal material and the preparation of marsdenia tenacissima is controlled by using the fingerprints of the phenolic acids and the steroid saponins, so that the identity, the producing area and the quality of the marsdenia tenacissima are identified and controlled, thus the quality of the medicinal material and the preparation of the marsdenia tenacissima has an actually controllable standard; stable quality of the medicinal material and the preparation of the marsdenia tenacissima is ensured. The method for controlling the quality of the marsdenia tenacissima through measuring the fingerprints of the phenolic acids and the steroid saponins in the medicinal material and the preparation of marsdenia tenacissima is simple, stable and reliable to operate.

The invention relates to a determination method for radix curcumae fingerprint and a standard fingerprint thereof. The determination method comprises the following steps of preparing of a test samplesolution, preparing of a reference sample solution, determining by gas chromatography, and determining by liquid chromatography, so as to obtain a chromatogram map; contrasting the obtained standard radix curcumae gas fingerprint and the liquid fingerprint, wherein the qualified product is obtained when the obtained standard radix curcumae gas fingerprint is consistent with the liquid fingerprint.The determination method has the advantages that the determination method is simple, rapid, accurate, stable and reliable, and is used for controlling the quality of the radix curcumae; the quality of the radix curcumae can be more comprehensively, objectively and scientifically evaluated; the validity of the preparation is guaranteed.

The invention provides a detection method for a specific chromatogram of medicinal preparation. The method comprises the following steps: 1) preparing a test solution; 2) preparing a reference substance solution; and 3) carrying out determination. The invention further provides application of the detection method of the specific chromatogram of the medicinal preparation in quality detection of the medicinal preparation, and a construction method of the standard specific chromatogram of the medicinal preparation, and a quality control method. The invention further provides a screening method of the specific chromatograms of multiple medicinal materials in the medicinal preparation. According to the method for detecting the specific chromatogram of the pharmaceutical preparation and the application of the method, the specific chromatogram of the pharmaceutical preparation containing 17 characteristic peaks can be obtained, 10 characteristic peaks in the specific chromatogram can be subjected to positioning attribution, the method is good in precision, stability and reproducibility, it is guaranteed that the amount of effective components in the pharmaceutical preparation is relatively stable, therefore, safety and effectiveness of clinical medication are guaranteed.