Detection method for fingerprint of radix puerariae assorted Chinese herbal tea
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A technology of fingerprint spectrum and detection method, which is applied in the detection field of traditional Chinese medicine substitute tea, can solve the problem of few quality detection methods of Pueraria Babao tea, and achieve the effect of simple method, good reproducibility and avoiding one-sidedness.
Active Publication Date: 2021-05-04
NANJING HAICHANG CHINESE MEDICINE GRPCO LTD +1
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[0004] At present, there are few qualit
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[0045] Embodiment 1 A kind of detection method of kudzu root eight-treasure tea fingerprint spectrum, comprises the following steps:
[0046] Step 1, the preparation of Pueraria babao tea test solution:
[0047] Take 8 batches of Pueraria babao tea samples 55g (Hovenia dulcis 10g, Pueraria 10g, Gardenia 5g, Chrysanthemum 4g, Cassia 5g, Lycium 5g, Orange peel 6g, Hawthorn 10g) and put them into a round bottom bottle after powdering. 500 mL of methanol solution, ultrasonically extracted for 30 min, filtered, the filtrate was placed in a volumetric flask, added methanol to constant volume, and passed through a 0.45 μm microporous membrane to obtain 8 batches of kudzu root eight treasure tea test solution;
[0048] Step 2, preparation of mixed reference solution:
[0049] Take accurately weighed puerarin and rutin reference substances, place them in a volumetric flask, dilute to the mark with methanol, shake well, and make a mixed reference solution containing 0.1008mg / mL puerari...
Example Embodiment
[0057] The methodological research of embodiment 2 fingerprint spectrum detection:
[0058] 1. Methodological investigation
[0059] 1.1 Inspection of precision
[0060] Get Pueraria babao tea sample 55g (Hovenia dulcis 10g, Pueraria 10g, Gardenia 5g, Chrysanthemum 4g, Cassia 5g, Lycium barbarum 5g, Orange peel 6g, Hawthorn 10g), according to the preparation method of the test sample in the above-mentioned embodiment 1, Need testing solution is prepared, continuous sampling 6 times, each injection volume 10 μ L, detects by above-mentioned embodiment 1 chromatographic conditions, measures its HPLC chromatogram, investigates 15 common fingerprint peaks in chromatogram, the result shows that, The retention time RSDs of the common fingerprint peaks were all less than 1.5%, and the common peak area RSDs were all less than 1.8%, indicating that the precision of the instrument was good.
[0061] 1.2 Stability investigation
[0062] Get Pueraria root Babao tea sample 5g, prepare ne...
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Abstract
The invention discloses a detection method for the fingerprint of a radix puerariae assorted Chinese herbal tea. The detection method comprises the following steps: 1, preparing a radix puerariae assorted Chinese herbal tea test solution; 2, preparing a mixed reference substance solution; 3, respectively and precisely sucking the mixed reference substance solution and the test solution, injecting the mixed reference substance solution and the test solution into a liquid chromatograph, and recording chromatogram maps; and 4, exporting the radix puerariae assorted Chinese herbal tea fingerprint, importing the fingerprint into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, selecting chromatographic peaks existing in chromatograms of different batches of radix puerariae assorted Chinese herbal tea as common peaks, generating a contrast fingerprint spectrum of the radix puerariae assorted Chinese herbal tea by using an average value calculation method, calculating the relative retention time and the relative peak area of each common peak, comparing the fingerprint of the radix puerariae assorted Chinese herbal tea with the spectrum of the mixed standard substance, and identifying main component peaks. The radix puerariae assorted Chinese herbal tea fingerprint provided by the invention can comprehensively and objectively characterize the quality of the radix puerariae assorted Chinese herbal tea. And the detection method has the advantages of simplicity, stability, high precision, good reproducibility and the like.
Description
technical field [0001] The invention relates to a detection method of a traditional Chinese medicine substitute tea, in particular to a detection method of a fingerprint of kudzu root eight-treasure tea. Background technique [0002] Nowadays, people's life is fast-paced, and work pressure is high. Overtime, socializing, and staying up late are commonplace. Unhealthy lifestyles put a huge load on our bodies. The liver is the most enduring organ in our body. Emotional stress, depression, overwork, and anger all damage the liver. The liver is the "chemical factory" of human metabolism. Toxic substances ingested, drugs that damage the liver, poisons and wastes produced in the body must be detoxified by the liver. Because the liver has no pain-sensing nerves, there is nothing wrong with it Clear symptoms, which is why people often ignore its health status. Minor ailments such as unexplained fatigue, dry eyes, insomnia, dreaminess, dry mouth, memory loss, etc., are likely to be...
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